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The map label for this gene is mraW

Identifier: 148651949

GI number: 148651949

Start: 158286

End: 159305

Strand: Direct

Name: mraW

Synonym: PsycPRwf_0132

Alternate gene names: 148651949

Gene position: 158286-159305 (Clockwise)

Preceding gene: 148651947

Following gene: 148651950

Centisome position: 5.31

GC content: 51.37

Gene sequence:

>1020_bases
GTGACCGATGTCCCTACCTCCGCACCACGCTCCACCTCAAAATCAGAGTTTAGCCACGAAACGGTGTTACTGTTTGAAAC
GGTAGCGGCAGTCTTGGGCGAAAAGTCGCTCAATGATAACACCCCCAAGCATGACGGGATTTATGTCGATGCGACCTTTG
GCCGTGGCGGGCACAGCAAGCTTTTGCTTAACTATTTAAGTGATGATGCCAAGCTGTTTGTGTTCGATAAAGATCCAGAG
GCCATTGATGTGGCCAATGATTTGGCCGCAGAAGATAAGCGGGTGATAGTGGTACACGACAGCTTTGCTGATATGACGCA
GTGCTTAAATAGCCGCGGGGTGAGCCAAGTTGACGGTATTATGGCTGATTTGGGCGTCTCCTCACCACAGCTTGATGATG
GCAGCCGCGGCTTTAGCTTTATGCGTGATGGTGCCATTGATATGCGTATGGACACCAGCCGAGGACAGCCGGTAGGCGAA
TGGCTACAAACAGTGGATGAAGAGACGTTGGCCGATGTGTTGTACGATTTCGGTGAGGAGCGTCACAGCCGACGTATTGC
TCGTGCCATCAAGGGCATGACGCAGTACACCTCGACTTTAGAGCTTGCGGAGGTGATTAAGCAGGCGCACCCCAAGTGGC
AAAAAGGCAAACATCCGGCGACCCAAAGCTTCCAAGCGATGCGTATCTTTATTAACAATGAGCTGGGCGATATAGATAGC
TTTTTGGCACAAAGCCTAACGCTGCTTGCCCCTCAAGGTCAGCTTGCAGTGATCAGCTTTCACTCTTTAGAAGACCGCCG
CATTAAGCAGTTTTTGCAGCGCCACAGCCGAGGTCAGTATCCTGAAGATGAAAACTTACCGATGCCGCCGCAGCGTCCAC
GTTATTTTAGTAAGCCCAAACGTATCGCACCAAGCAAAGCGGAGGTCACCGTGAATAATCGCTCAAGAAGTGCTTGGTTA
AGAGTAGCAACACGCACCGATACCCCATATAATACCGACCCTAGCCCGCAACACAGCTAG

Upstream 100 bases:

>100_bases
CCTTATTTATTACTTGCCTGTTTTAACCACACTGATAAGCTAAGTATGCGATGTTGATGGCTATCAGGTTGGTTTACGTT
TTTTAGGAATACTAGCTACT

Downstream 100 bases:

>100_bases
GGCGAACCTAGGCAATATAAATTCAGTTAAATGGATGCGCTAACCGGGGTATGATTAAGTCAATTAACGCCCCAAAACTC
CATAAAGCGGCTGTTTTACT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTDVPTSAPRSTSKSEFSHETVLLFETVAAVLGEKSLNDNTPKHDGIYVDATFGRGGHSKLLLNYLSDDAKLFVFDKDPE
AIDVANDLAAEDKRVIVVHDSFADMTQCLNSRGVSQVDGIMADLGVSSPQLDDGSRGFSFMRDGAIDMRMDTSRGQPVGE
WLQTVDEETLADVLYDFGEERHSRRIARAIKGMTQYTSTLELAEVIKQAHPKWQKGKHPATQSFQAMRIFINNELGDIDS
FLAQSLTLLAPQGQLAVISFHSLEDRRIKQFLQRHSRGQYPEDENLPMPPQRPRYFSKPKRIAPSKAEVTVNNRSRSAWL
RVATRTDTPYNTDPSPQHS

Sequences:

>Translated_339_residues
MTDVPTSAPRSTSKSEFSHETVLLFETVAAVLGEKSLNDNTPKHDGIYVDATFGRGGHSKLLLNYLSDDAKLFVFDKDPE
AIDVANDLAAEDKRVIVVHDSFADMTQCLNSRGVSQVDGIMADLGVSSPQLDDGSRGFSFMRDGAIDMRMDTSRGQPVGE
WLQTVDEETLADVLYDFGEERHSRRIARAIKGMTQYTSTLELAEVIKQAHPKWQKGKHPATQSFQAMRIFINNELGDIDS
FLAQSLTLLAPQGQLAVISFHSLEDRRIKQFLQRHSRGQYPEDENLPMPPQRPRYFSKPKRIAPSKAEVTVNNRSRSAWL
RVATRTDTPYNTDPSPQHS
>Mature_338_residues
TDVPTSAPRSTSKSEFSHETVLLFETVAAVLGEKSLNDNTPKHDGIYVDATFGRGGHSKLLLNYLSDDAKLFVFDKDPEA
IDVANDLAAEDKRVIVVHDSFADMTQCLNSRGVSQVDGIMADLGVSSPQLDDGSRGFSFMRDGAIDMRMDTSRGQPVGEW
LQTVDEETLADVLYDFGEERHSRRIARAIKGMTQYTSTLELAEVIKQAHPKWQKGKHPATQSFQAMRIFINNELGDIDSF
LAQSLTLLAPQGQLAVISFHSLEDRRIKQFLQRHSRGQYPEDENLPMPPQRPRYFSKPKRIAPSKAEVTVNNRSRSAWLR
VATRTDTPYNTDPSPQHS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=344, Percent_Identity=33.7209302325581, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI165377202, Length=197, Percent_Identity=34.5177664974619, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786270, Length=322, Percent_Identity=46.8944099378882, Blast_Score=276, Evalue=2e-75,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=33.7349397590361, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=33.7349397590361, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PSYWF (A5WBQ1)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279042.1
- ProteinModelPortal:   A5WBQ1
- SMR:   A5WBQ1
- STRING:   A5WBQ1
- GeneID:   5206744
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0132
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- BioCyc:   PSP56811:PSYCPRWF_0132-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 37952; Mature: 37821

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: BINDING 76-76 BINDING 102-102 BINDING 123-123 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPTSAPRSTSKSEFSHETVLLFETVAAVLGEKSLNDNTPKHDGIYVDATFGRGGHSK
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHH
LLLNYLSDDAKLFVFDKDPEAIDVANDLAAEDKRVIVVHDSFADMTQCLNSRGVSQVDGI
HHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHH
MADLGVSSPQLDDGSRGFSFMRDGAIDMRMDTSRGQPVGEWLQTVDEETLADVLYDFGEE
HHHCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHSRRIARAIKGMTQYTSTLELAEVIKQAHPKWQKGKHPATQSFQAMRIFINNELGDIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCCHHHH
FLAQSLTLLAPQGQLAVISFHSLEDRRIKQFLQRHSRGQYPEDENLPMPPQRPRYFSKPK
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
RIAPSKAEVTVNNRSRSAWLRVATRTDTPYNTDPSPQHS
CCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDVPTSAPRSTSKSEFSHETVLLFETVAAVLGEKSLNDNTPKHDGIYVDATFGRGGHSK
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHH
LLLNYLSDDAKLFVFDKDPEAIDVANDLAAEDKRVIVVHDSFADMTQCLNSRGVSQVDGI
HHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHHHHHH
MADLGVSSPQLDDGSRGFSFMRDGAIDMRMDTSRGQPVGEWLQTVDEETLADVLYDFGEE
HHHCCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RHSRRIARAIKGMTQYTSTLELAEVIKQAHPKWQKGKHPATQSFQAMRIFINNELGDIDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCCHHHH
FLAQSLTLLAPQGQLAVISFHSLEDRRIKQFLQRHSRGQYPEDENLPMPPQRPRYFSKPK
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
RIAPSKAEVTVNNRSRSAWLRVATRTDTPYNTDPSPQHS
CCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA