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The map label for this gene is hisH [H]

Identifier: 148651878

GI number: 148651878

Start: 74243

End: 74878

Strand: Direct

Name: hisH [H]

Synonym: PsycPRwf_0061

Alternate gene names: 148651878

Gene position: 74243-74878 (Clockwise)

Preceding gene: 148651877

Following gene: 148651879

Centisome position: 2.49

GC content: 48.27

Gene sequence:

>636_bases
ATGACCAAAATTGCATTGTTAGACTATGGCATGGGCAACCTCCACTCTGCCAGCAAAGCGTTGGCTGCGGTGGGGGCTGA
GGTATCTATTACCAATGATCCAAACATCATCCGTGCAGCAGACAAAATCGTCTTTCCAGGCGTGGGGGCAATGCGCGACT
GTATGGCACAGATGCGTGAGCAAGGGATTGATGAGGTGGTCAAAGAAGCGGCCTTTAATAAGCCGGTGATGGCAATTTGT
GTCGGCATGCAAGCGCTGTTTACGGAGTCGCTTGAGTTTGAACATACCGAGTGTTTGGATATCCTGCAAGGTCAGGTAAA
ACAGTTTGACCCTAGCTGGATTGACCCTAAAGGCGAGACCATTAAGATTCCTCATATGGGCTGGAATACCATTGAGGGCA
TGGATTTGAGTCATCCGCTGTGGCAAGGTATTGATGAGCATGCGCATTTTTATTTCGTACACAGCTATTACTGCGCCCCA
GAAGATAACTCTGTGGTGGCGGCGGTGTGTGATTACGGTCAGCCGTTTTGTGCCAGCGTGTTAAAAGACAATCTGTTTGC
GACCCAGTTTCACCCAGAAAAAAGCCACAATGATGGGCTACAACTGCTAAAAAACTTCGTTGAATGGAACATCTAA

Upstream 100 bases:

>100_bases
TGTACTTTTAAGGCCTTTGCTCGTGCGCTGCGTATGGCTTGTGAGTATGATGAGCGTGCGCTAAACACGCTACCTTCTAC
CAAAGAGGCATTGTAACTAT

Downstream 100 bases:

>100_bases
CCGAACTGGCAACATGACAATCCCAATAACAATTCCAATAACAAGGCCAATAGCAATCAGGCTCGCAGCTAAAAAGACAG
CAGCTAAAAAAACAGCCACA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKIALLDYGMGNLHSASKALAAVGAEVSITNDPNIIRAADKIVFPGVGAMRDCMAQMREQGIDEVVKEAAFNKPVMAIC
VGMQALFTESLEFEHTECLDILQGQVKQFDPSWIDPKGETIKIPHMGWNTIEGMDLSHPLWQGIDEHAHFYFVHSYYCAP
EDNSVVAAVCDYGQPFCASVLKDNLFATQFHPEKSHNDGLQLLKNFVEWNI

Sequences:

>Translated_211_residues
MTKIALLDYGMGNLHSASKALAAVGAEVSITNDPNIIRAADKIVFPGVGAMRDCMAQMREQGIDEVVKEAAFNKPVMAIC
VGMQALFTESLEFEHTECLDILQGQVKQFDPSWIDPKGETIKIPHMGWNTIEGMDLSHPLWQGIDEHAHFYFVHSYYCAP
EDNSVVAAVCDYGQPFCASVLKDNLFATQFHPEKSHNDGLQLLKNFVEWNI
>Mature_210_residues
TKIALLDYGMGNLHSASKALAAVGAEVSITNDPNIIRAADKIVFPGVGAMRDCMAQMREQGIDEVVKEAAFNKPVMAICV
GMQALFTESLEFEHTECLDILQGQVKQFDPSWIDPKGETIKIPHMGWNTIEGMDLSHPLWQGIDEHAHFYFVHSYYCAPE
DNSVVAAVCDYGQPFCASVLKDNLFATQFHPEKSHNDGLQLLKNFVEWNI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=40.9756097560976, Blast_Score=150, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319725, Length=220, Percent_Identity=32.2727272727273, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23490; Mature: 23359

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIALLDYGMGNLHSASKALAAVGAEVSITNDPNIIRAADKIVFPGVGAMRDCMAQMRE
CCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEEHHHHEECCCCCHHHHHHHHHHH
QGIDEVVKEAAFNKPVMAICVGMQALFTESLEFEHTECLDILQGQVKQFDPSWIDPKGET
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCE
IKIPHMGWNTIEGMDLSHPLWQGIDEHAHFYFVHSYYCAPEDNSVVAAVCDYGQPFCASV
EEECCCCCCCCCCCCCCCHHHHCHHHCCEEEEEEEEEECCCCCCEEEEEECCCCHHHHHH
LKDNLFATQFHPEKSHNDGLQLLKNFVEWNI
HHCCCEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIALLDYGMGNLHSASKALAAVGAEVSITNDPNIIRAADKIVFPGVGAMRDCMAQMRE
CEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEEHHHHEECCCCCHHHHHHHHHHH
QGIDEVVKEAAFNKPVMAICVGMQALFTESLEFEHTECLDILQGQVKQFDPSWIDPKGET
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCE
IKIPHMGWNTIEGMDLSHPLWQGIDEHAHFYFVHSYYCAPEDNSVVAAVCDYGQPFCASV
EEECCCCCCCCCCCCCCCHHHHCHHHCCEEEEEEEEEECCCCCCEEEEEECCCCHHHHHH
LKDNLFATQFHPEKSHNDGLQLLKNFVEWNI
HHCCCEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA