Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 148651820

GI number: 148651820

Start: 2984

End: 4159

Strand: Direct

Name: dnaN [H]

Synonym: PsycPRwf_0003

Alternate gene names: 148651820

Gene position: 2984-4159 (Clockwise)

Preceding gene: 148651819

Following gene: 148651821

Centisome position: 0.1

GC content: 44.56

Gene sequence:

>1176_bases
ATGCAACTGTCTATCAATCGTGAACTGCTCTTAAAAGCCATTAATCTAATTGCTAAAGCAGCAGACAAACGCCATAACAT
GGTCGTTTTGGGTAACATTAAACTAGAGGTTACTGAGCAGCTACTGATTTTGACGGCCTCAGATCTAGAAGTAGAGCTGA
GTGCACAAGTTAAGCTTGGTAATGGTGCCACAGGTAGTATTGGTGCGACCACGCTGCCGGCATCTAAATTACATGAAATC
TGTAAGCTACTGCCTAAAGACACCATGATTAACCTTGCAGCGGAGAGTAATGAGCGCTGCGTGATTAATAGTGGCAAAAG
CCGTTTTGTACTGGGCACATTGCCAGCTGCAGACTTCCCAATATTGGGTAATCCGGCCAATATTACGCCGCTCACCTTAA
GCCGTAATGTGCTGATGGATCTTATGGCTAAGACGCAGTTTGCGATGGCCATTCAAGATGTTCGTTATTACCTTACCGGT
ATGTTATTTGAAGTGTCGCAGCGTCAGCTCACCACGGTTGCCACAGACGGTCACCGTTTGGCTTTGGCTCGTAGTGTTAT
TGAAGTGGCAGATGATTTTAGCATGCAGGCTATTTTACCGCGCAAAGCAGTTATTGAGCTTGAGCGTTTGGCCAATGAGC
TGGGTAAATTAGTGGGGGGTAATGATGGTAACCTCACCTTAAGCTTTGGCCGTGAGTTCTTACAAGTAAGCCTACCTTTT
GGTAATGTGGACAGCACAGGCCAGATTGATGACTCAGTGCGTGTGACCTTTACGGCACGCTTAATAGATGGCAAATTCCC
TGATTATCGCCGAGTTATGCCTAATAATACTGATAAAATAGCACTGGCTAATAAAGAACAAATGACCGATGTCTTAAGAC
GGGTTTCTATCTTAAGTAATGAAAAGTCGCGCGGGGTTATTTTTAAATTCTCGCAAGATGGCAATGTCGAAGTACGTGCC
AATAATGCTGAGCAGGATGAAGCAGTCGAAGCCTTACAAGTAAAATATCAAGGTGCCCCGATTGAGTTGTCGTTTAACGC
AGCCTATTTACAAGATGTACTCGATGTCTTAGGCGGCGATGTGCAACTACACATGAGTCAAGCTAATAGCTCGGTATTGG
TAAATCAGCTCAATGACAGCCAACACCAATACGTCATTATGCCCATGCGTATTTAA

Upstream 100 bases:

>100_bases
TTATAACGGCATCCTTGTGTCATAGTGAGTGCTAAGCTTTATAATCAATACTAAATTGGCTATTAACTTATATTAAGACC
CCATTAAAGAGTCCTATATT

Downstream 100 bases:

>100_bases
CCGCTTATGCTGTCCCATACTATTAAAAGCTAACGCCATAATAAAAAAGCAAAACTTAAGGTTTTGCTTTTTTTATGGGG
ATAAGCTGTGGATAACTTTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQLSINRELLLKAINLIAKAADKRHNMVVLGNIKLEVTEQLLILTASDLEVELSAQVKLGNGATGSIGATTLPASKLHEI
CKLLPKDTMINLAAESNERCVINSGKSRFVLGTLPAADFPILGNPANITPLTLSRNVLMDLMAKTQFAMAIQDVRYYLTG
MLFEVSQRQLTTVATDGHRLALARSVIEVADDFSMQAILPRKAVIELERLANELGKLVGGNDGNLTLSFGREFLQVSLPF
GNVDSTGQIDDSVRVTFTARLIDGKFPDYRRVMPNNTDKIALANKEQMTDVLRRVSILSNEKSRGVIFKFSQDGNVEVRA
NNAEQDEAVEALQVKYQGAPIELSFNAAYLQDVLDVLGGDVQLHMSQANSSVLVNQLNDSQHQYVIMPMRI

Sequences:

>Translated_391_residues
MQLSINRELLLKAINLIAKAADKRHNMVVLGNIKLEVTEQLLILTASDLEVELSAQVKLGNGATGSIGATTLPASKLHEI
CKLLPKDTMINLAAESNERCVINSGKSRFVLGTLPAADFPILGNPANITPLTLSRNVLMDLMAKTQFAMAIQDVRYYLTG
MLFEVSQRQLTTVATDGHRLALARSVIEVADDFSMQAILPRKAVIELERLANELGKLVGGNDGNLTLSFGREFLQVSLPF
GNVDSTGQIDDSVRVTFTARLIDGKFPDYRRVMPNNTDKIALANKEQMTDVLRRVSILSNEKSRGVIFKFSQDGNVEVRA
NNAEQDEAVEALQVKYQGAPIELSFNAAYLQDVLDVLGGDVQLHMSQANSSVLVNQLNDSQHQYVIMPMRI
>Mature_391_residues
MQLSINRELLLKAINLIAKAADKRHNMVVLGNIKLEVTEQLLILTASDLEVELSAQVKLGNGATGSIGATTLPASKLHEI
CKLLPKDTMINLAAESNERCVINSGKSRFVLGTLPAADFPILGNPANITPLTLSRNVLMDLMAKTQFAMAIQDVRYYLTG
MLFEVSQRQLTTVATDGHRLALARSVIEVADDFSMQAILPRKAVIELERLANELGKLVGGNDGNLTLSFGREFLQVSLPF
GNVDSTGQIDDSVRVTFTARLIDGKFPDYRRVMPNNTDKIALANKEQMTDVLRRVSILSNEKSRGVIFKFSQDGNVEVRA
NNAEQDEAVEALQVKYQGAPIELSFNAAYLQDVLDVLGGDVQLHMSQANSSVLVNQLNDSQHQYVIMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=392, Percent_Identity=40.3061224489796, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42921; Mature: 42921

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSINRELLLKAINLIAKAADKRHNMVVLGNIKLEVTEQLLILTASDLEVELSAQVKLG
CEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEC
NGATGSIGATTLPASKLHEICKLLPKDTMINLAAESNERCVINSGKSRFVLGTLPAADFP
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCCC
ILGNPANITPLTLSRNVLMDLMAKTQFAMAIQDVRYYLTGMLFEVSQRQLTTVATDGHRL
EECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
ALARSVIEVADDFSMQAILPRKAVIELERLANELGKLVGGNDGNLTLSFGREFLQVSLPF
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCC
GNVDSTGQIDDSVRVTFTARLIDGKFPDYRRVMPNNTDKIALANKEQMTDVLRRVSILSN
CCCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCC
EKSRGVIFKFSQDGNVEVRANNAEQDEAVEALQVKYQGAPIELSFNAAYLQDVLDVLGGD
CCCCCEEEEECCCCCEEEEECCCCHHHHHHHHEEEECCCCEEEEECHHHHHHHHHHHCCC
VQLHMSQANSSVLVNQLNDSQHQYVIMPMRI
EEEEEECCCCEEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MQLSINRELLLKAINLIAKAADKRHNMVVLGNIKLEVTEQLLILTASDLEVELSAQVKLG
CEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEC
NGATGSIGATTLPASKLHEICKLLPKDTMINLAAESNERCVINSGKSRFVLGTLPAADFP
CCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCCC
ILGNPANITPLTLSRNVLMDLMAKTQFAMAIQDVRYYLTGMLFEVSQRQLTTVATDGHRL
EECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHH
ALARSVIEVADDFSMQAILPRKAVIELERLANELGKLVGGNDGNLTLSFGREFLQVSLPF
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCC
GNVDSTGQIDDSVRVTFTARLIDGKFPDYRRVMPNNTDKIALANKEQMTDVLRRVSILSN
CCCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCC
EKSRGVIFKFSQDGNVEVRANNAEQDEAVEALQVKYQGAPIELSFNAAYLQDVLDVLGGD
CCCCCEEEEECCCCCEEEEECCCCHHHHHHHHEEEECCCCEEEEECHHHHHHHHHHHCCC
VQLHMSQANSSVLVNQLNDSQHQYVIMPMRI
EEEEEECCCCEEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]