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The map label for this gene is pinE [C]

Identifier: 148550716

GI number: 148550716

Start: 178072

End: 178989

Strand: Direct

Name: pinE [C]

Synonym: Swit_5280

Alternate gene names: 148550716

Gene position: 178072-178989 (Clockwise)

Preceding gene: 148550715

Following gene: 148550717

Centisome position: 57.4

GC content: 65.58

Gene sequence:

>918_bases
TTGATCGGCTATGCCCGCGTATCGACCGAAGATCAGGACACGGCCAGCCAACGCCTTGCGCTGGAGCAGCAGGGTTGCGC
CCTCATCTTCGAGGACAAGGCCAGCGGGGGAAGCCGGGACCGCCCGAACCTCGCCAGGGCGTTGGCAAGGGTAGGGGAGG
GCGACACCCTCATTGTCGTGCGGATCGATCGATTGGCGCGCTCGCTCGTCCATCTGCTGGAGATCGTCGAGCAGCTGCGG
GCGAAGGGCGCCTACTTTCGCTCGATCAACGACCCGATCGACACCTCGAGCGCGCAAGGCATGCTGATGATCCAGATGCT
GGGCGCCTTTGCCGAATTCGAACGCGCCCTTATCCGAGAACGCACGCGGGCAGGGCTTGCCGCGGCGATGGCGAAGGGCG
CCAAGCCCGGCAATCCCAAGATGCGCGCGCGCGATCCGGCGGCAATCGCGGATATCGGCTACGCGCACAAAGAGCGTTAT
CTGCACGCCTTGATCGACGATCGCCACCGCTGGCTGCCGACCGTAGAGCGCCTGCGACCCCATCTTCCCTGGTCGATAGT
CTTGCGCCAGATCCGCGCGATAAAGCCCCCCGTCCGGTCATTCAGTGAGCGCACGCTGGTGAAGGCCTGCAGGGCCCTGG
TCAAAGCCGGTTATGCTGACGCCGTTATCCTTCAGGCCGCGCCGCGCCTGCCACCGGATACCCGCGTCGCGCGCCTGGTG
GCGGACAGGTTGAAGACATATCCGGGAGCGTCTCTGCGCGACCTTGCGGGCTGGCTCAGCAGAGACTTGCGCGAACCTAC
GCCACGCGGCGGCATACACTGGTCGGCTGAGGGCGTGCGGCGTGTTCTGGAACGGGCGAGGGAGCTCGGGCTTCTCCTAG
ATCGCGAGGCGTCTTCGGACCTCTGTCTGATCGCCTAA

Upstream 100 bases:

>100_bases
TCGAATCCTAGGGGACGGGGCTGGCCAAAACCGGCACTGGCGTTTATCAAACGGGAGCAACCCCTCGATTGCTCCCGTTT
GATGGAATGTGACAGTGGCT

Downstream 100 bases:

>100_bases
CCAGAGCTCGATAATGTGCCGCGTCTTGTGGGGCGCGGCACATGGCGCGGCACCTGAAGTTGAAGGGGCGCGCCGCGAGT
TGGCCGGCGACCGGATTGTT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MIGYARVSTEDQDTASQRLALEQQGCALIFEDKASGGSRDRPNLARALARVGEGDTLIVVRIDRLARSLVHLLEIVEQLR
AKGAYFRSINDPIDTSSAQGMLMIQMLGAFAEFERALIRERTRAGLAAAMAKGAKPGNPKMRARDPAAIADIGYAHKERY
LHALIDDRHRWLPTVERLRPHLPWSIVLRQIRAIKPPVRSFSERTLVKACRALVKAGYADAVILQAAPRLPPDTRVARLV
ADRLKTYPGASLRDLAGWLSRDLREPTPRGGIHWSAEGVRRVLERARELGLLLDREASSDLCLIA

Sequences:

>Translated_305_residues
MIGYARVSTEDQDTASQRLALEQQGCALIFEDKASGGSRDRPNLARALARVGEGDTLIVVRIDRLARSLVHLLEIVEQLR
AKGAYFRSINDPIDTSSAQGMLMIQMLGAFAEFERALIRERTRAGLAAAMAKGAKPGNPKMRARDPAAIADIGYAHKERY
LHALIDDRHRWLPTVERLRPHLPWSIVLRQIRAIKPPVRSFSERTLVKACRALVKAGYADAVILQAAPRLPPDTRVARLV
ADRLKTYPGASLRDLAGWLSRDLREPTPRGGIHWSAEGVRRVLERARELGLLLDREASSDLCLIA
>Mature_305_residues
MIGYARVSTEDQDTASQRLALEQQGCALIFEDKASGGSRDRPNLARALARVGEGDTLIVVRIDRLARSLVHLLEIVEQLR
AKGAYFRSINDPIDTSSAQGMLMIQMLGAFAEFERALIRERTRAGLAAAMAKGAKPGNPKMRARDPAAIADIGYAHKERY
LHALIDDRHRWLPTVERLRPHLPWSIVLRQIRAIKPPVRSFSERTLVKACRALVKAGYADAVILQAAPRLPPDTRVARLV
ADRLKTYPGASLRDLAGWLSRDLREPTPRGGIHWSAEGVRRVLERARELGLLLDREASSDLCLIA

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=142, Percent_Identity=52.112676056338, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787638, Length=146, Percent_Identity=37.6712328767123, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787827, Length=146, Percent_Identity=37.6712328767123, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 33883; Mature: 33883

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGYARVSTEDQDTASQRLALEQQGCALIFEDKASGGSRDRPNLARALARVGEGDTLIVV
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEE
RIDRLARSLVHLLEIVEQLRAKGAYFRSINDPIDTSSAQGMLMIQMLGAFAEFERALIRE
EHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTRAGLAAAMAKGAKPGNPKMRARDPAAIADIGYAHKERYLHALIDDRHRWLPTVERLRP
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
HLPWSIVLRQIRAIKPPVRSFSERTLVKACRALVKAGYADAVILQAAPRLPPDTRVARLV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHH
ADRLKTYPGASLRDLAGWLSRDLREPTPRGGIHWSAEGVRRVLERARELGLLLDREASSD
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHCCCEECCCCCC
LCLIA
EEEEC
>Mature Secondary Structure
MIGYARVSTEDQDTASQRLALEQQGCALIFEDKASGGSRDRPNLARALARVGEGDTLIVV
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEE
RIDRLARSLVHLLEIVEQLRAKGAYFRSINDPIDTSSAQGMLMIQMLGAFAEFERALIRE
EHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTRAGLAAAMAKGAKPGNPKMRARDPAAIADIGYAHKERYLHALIDDRHRWLPTVERLRP
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
HLPWSIVLRQIRAIKPPVRSFSERTLVKACRALVKAGYADAVILQAAPRLPPDTRVARLV
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHH
ADRLKTYPGASLRDLAGWLSRDLREPTPRGGIHWSAEGVRRVLERARELGLLLDREASSD
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHCCCEECCCCCC
LCLIA
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]