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The map label for this gene is traD [H]

Identifier: 148550599

GI number: 148550599

Start: 54040

End: 56346

Strand: Reverse

Name: traD [H]

Synonym: Swit_5160

Alternate gene names: 148550599

Gene position: 56346-54040 (Counterclockwise)

Preceding gene: 148550600

Following gene: 148550598

Centisome position: 18.16

GC content: 65.11

Gene sequence:

>2307_bases
ATGGCGCGTGAGGACATCCGCACGGACGGGCGTCCCGTCCCGCTCACCCATCATTCGGCGCGCGGCCGCGTGCAGCGCAA
TGCCGGCAATTTCACCCGCGGCAGCCAGCTACTCACCCATGAGATGCTGATGTGGTTCTCGGGCGCCAAGCTGCCCTTCA
TCCTCTGGTTCTTCGCCTTTCTCGCCGCCTGGTTCATCATCATGTCGCTCAAGCTCGACGAGCATGGTTTCCAGCTCGTC
TGCATGAAGGTCTATGCGACCCTCTGGAACTGGATCGACCTCAATCCGAACAAGCGCGTCAACGTCACGCTGCCGAACGG
CGACCTCATGCGCACCGTGATGAAAGCGGTGCCCTATATTCCCGAGGTCATCACAGCCTGGGCCGTCGCGATGCGGGGCC
TCATCGGCGCTTTCCTGATCTCGGTCTTCATCACCATTCCGGCCGCCATCTGGTTCGTCGACATCTCGCATCGGCGCGGC
CGTTCGATCCTTCAGGAACGGCATGAACGCGGCGCCATGCTGGTTGATCGCAGCGTGCTTCTGGTGGAGATCAACGAGCA
TAACCGGGTGAAGTTCGAAGAGGAGGCGGCCGACCTCTTCCCCGGCCTGTCCTCGCGGCAGGTTCTCGCGCTCCCGTTCC
GAGCGCGCAAGGAAGCCGGCATCCATCATCCCTACACGCTCGCGGGCATCCCCTTTCCGCACCGCACCGAACAATCCCAT
GCGATGCTCATCGGCACCACCGGATCAGGCAAGACGACCGAGCTCAGAAGCCTCGTCACCCAGATGCGCGAGCGCCAGGA
CACGGCCGTCATCTTCGATCTCACCGGCGCCTATGTCGAAGCCTTCTACGATCCCACACGCGACACGATCCTCAATCCCA
TGGACGCGCGCTGCCCGGCCTGGTCGATCTTCAACGATTGCCGCACCCACAGCGAATTCACGGCCGCCGCGGCGGCGCTA
ATCCCTTCGGATGGCGGTTCGTCCGAACCCTTCTGGGCGCTCGCAGCGCGCACCCTCTTCATCGAGATGTGCATTCGCCT
GATGGAGCGGGGGCAGACCAGCAATCTGGCGCTGTCCGAGAACCTGATGACGGCCGACCTGAAGCGCGTCCACCGCTTCC
TCGCCAACACCATCGCCGACCCGCTCACTGCCCCCGAAGCGGCCCGCATGGCGGAATCGATCCGCGCCGTCTTCAACACC
AATGCGCAGGTCCTTCGCTTCCTTCCCGACGAGGGCGAATCCTTCTCGATCCGCGACTGGATCACGCGGGACAAGAAGCC
CGGATCGATCCTGTTCGTGACGTCGAACTATGTCGATCTGCCGATGAACCGGGCGCTGCTCACGCTGTGGATGGATCTCG
CGATCAACCGGCTGATGACGCTGCCGCGCACCCGCAGCTTGCGCACCTGGTTCATGTTCGACGAGCTTGGCGCACTGCAT
CGCCTTCCGGCGATCGAGAATGGTCTCCAGACGGCGCGCGCGTTTGGCGGCGCGATGATCCTGGGCATCCACAGCTTCGA
GAAGCTGGTCGAAGTCTATGGCGAGCAAGGGGCACGCAATCTCGCCTCGCTCGCCCGCTCCAAGCTCATCCTCGCGACGG
CCGATCTCGACACGGCCGAGCAGTGCGCGCGCTACATCGGCAACCGCGAGGTTCGCCAGATGGATGAGGCCTATAGCTAC
GGCTACAATAACACCCGCGACGCTTCGACCCTGACCCCGCGCAAGCAGGTCGAACCACTCGTCATCGCCGATGACATCAC
CAACCTGCCCTCGATGCACGGCTTCGTGAAATTCCCCGACGGCTTCCCCGCCGCGCGCATCCTGCTCGAGTGGAAGGACT
ATCCCCAGGTCGCCCAGGGCTTCGTCCAACGGCCCGATGTCGGGCCGGTACGCTCAAGGCGCGGAGAGGAGGTGTTCGAG
GAAGACGGGGCAGGGGAGGCGGGCGGGCGCGACGGCTCGTTGCAGGTCGTGGAGGAAGTGGCCGAGATCACCAATCTCGC
CCGCGATCTCGCCGCACGGATCCTGTCGGCCGAGGTCGAGGAGGAGGACGATGCCGCCCGCCGCGCGGCGCAGCGGGCGG
ACGACCGGGACGAACAGGCGACGCCTGGCACGCATAGCGCGGACAGGGCGCAGGCGGCGCGCGCCAGCGGCGAGGAACAG
CCGGTTTCCGCGCGCGACCGGGACGATCGTCGTGATGGCCACCGGCACGGGGAGACGAGCCCACAGGTCGAGGACCAGAC
CCTGATCGAGCTGCGCCACGACTTCTCCACCGGCCGCGAGGATGACGGCATGGACATGGGCATCTGA

Upstream 100 bases:

>100_bases
GAGTTCCAGCATCTCGCGCTCGACATCATCATCCGCGAACAATTCCCCGAATATCGCGACCGCATCATCGCGGAAACCGA
CAAACGTCTGGAGCAATATC

Downstream 100 bases:

>100_bases
TGCTCTCGGTCGCCGGCGTCCGCTCCGCTTCCGGTGCCGCGAACTATTTCGCGAAGGACGACTATTACACGGTCGAGGGC
TCGTCCGAGATCAGCGCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MAREDIRTDGRPVPLTHHSARGRVQRNAGNFTRGSQLLTHEMLMWFSGAKLPFILWFFAFLAAWFIIMSLKLDEHGFQLV
CMKVYATLWNWIDLNPNKRVNVTLPNGDLMRTVMKAVPYIPEVITAWAVAMRGLIGAFLISVFITIPAAIWFVDISHRRG
RSILQERHERGAMLVDRSVLLVEINEHNRVKFEEEAADLFPGLSSRQVLALPFRARKEAGIHHPYTLAGIPFPHRTEQSH
AMLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYDPTRDTILNPMDARCPAWSIFNDCRTHSEFTAAAAAL
IPSDGGSSEPFWALAARTLFIEMCIRLMERGQTSNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNT
NAQVLRFLPDEGESFSIRDWITRDKKPGSILFVTSNYVDLPMNRALLTLWMDLAINRLMTLPRTRSLRTWFMFDELGALH
RLPAIENGLQTARAFGGAMILGIHSFEKLVEVYGEQGARNLASLARSKLILATADLDTAEQCARYIGNREVRQMDEAYSY
GYNNTRDASTLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARILLEWKDYPQVAQGFVQRPDVGPVRSRRGEEVFE
EDGAGEAGGRDGSLQVVEEVAEITNLARDLAARILSAEVEEEDDAARRAAQRADDRDEQATPGTHSADRAQAARASGEEQ
PVSARDRDDRRDGHRHGETSPQVEDQTLIELRHDFSTGREDDGMDMGI

Sequences:

>Translated_768_residues
MAREDIRTDGRPVPLTHHSARGRVQRNAGNFTRGSQLLTHEMLMWFSGAKLPFILWFFAFLAAWFIIMSLKLDEHGFQLV
CMKVYATLWNWIDLNPNKRVNVTLPNGDLMRTVMKAVPYIPEVITAWAVAMRGLIGAFLISVFITIPAAIWFVDISHRRG
RSILQERHERGAMLVDRSVLLVEINEHNRVKFEEEAADLFPGLSSRQVLALPFRARKEAGIHHPYTLAGIPFPHRTEQSH
AMLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYDPTRDTILNPMDARCPAWSIFNDCRTHSEFTAAAAAL
IPSDGGSSEPFWALAARTLFIEMCIRLMERGQTSNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNT
NAQVLRFLPDEGESFSIRDWITRDKKPGSILFVTSNYVDLPMNRALLTLWMDLAINRLMTLPRTRSLRTWFMFDELGALH
RLPAIENGLQTARAFGGAMILGIHSFEKLVEVYGEQGARNLASLARSKLILATADLDTAEQCARYIGNREVRQMDEAYSY
GYNNTRDASTLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARILLEWKDYPQVAQGFVQRPDVGPVRSRRGEEVFE
EDGAGEAGGRDGSLQVVEEVAEITNLARDLAARILSAEVEEEDDAARRAAQRADDRDEQATPGTHSADRAQAARASGEEQ
PVSARDRDDRRDGHRHGETSPQVEDQTLIELRHDFSTGREDDGMDMGI
>Mature_767_residues
AREDIRTDGRPVPLTHHSARGRVQRNAGNFTRGSQLLTHEMLMWFSGAKLPFILWFFAFLAAWFIIMSLKLDEHGFQLVC
MKVYATLWNWIDLNPNKRVNVTLPNGDLMRTVMKAVPYIPEVITAWAVAMRGLIGAFLISVFITIPAAIWFVDISHRRGR
SILQERHERGAMLVDRSVLLVEINEHNRVKFEEEAADLFPGLSSRQVLALPFRARKEAGIHHPYTLAGIPFPHRTEQSHA
MLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYDPTRDTILNPMDARCPAWSIFNDCRTHSEFTAAAAALI
PSDGGSSEPFWALAARTLFIEMCIRLMERGQTSNLALSENLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNTN
AQVLRFLPDEGESFSIRDWITRDKKPGSILFVTSNYVDLPMNRALLTLWMDLAINRLMTLPRTRSLRTWFMFDELGALHR
LPAIENGLQTARAFGGAMILGIHSFEKLVEVYGEQGARNLASLARSKLILATADLDTAEQCARYIGNREVRQMDEAYSYG
YNNTRDASTLTPRKQVEPLVIADDITNLPSMHGFVKFPDGFPAARILLEWKDYPQVAQGFVQRPDVGPVRSRRGEEVFEE
DGAGEAGGRDGSLQVVEEVAEITNLARDLAARILSAEVEEEDDAARRAAQRADDRDEQATPGTHSADRAQAARASGEEQP
VSARDRDDRRDGHRHGETSPQVEDQTLIELRHDFSTGREDDGMDMGI

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 86173; Mature: 86042

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREDIRTDGRPVPLTHHSARGRVQRNAGNFTRGSQLLTHEMLMWFSGAKLPFILWFFAF
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LAAWFIIMSLKLDEHGFQLVCMKVYATLWNWIDLNPNKRVNVTLPNGDLMRTVMKAVPYI
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHHHHHHHHCCCH
PEVITAWAVAMRGLIGAFLISVFITIPAAIWFVDISHRRGRSILQERHERGAMLVDRSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEECCEE
LVEINEHNRVKFEEEAADLFPGLSSRQVLALPFRARKEAGIHHPYTLAGIPFPHRTEQSH
EEEECCCCCEEEHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCCEEECCCCCCCCCCCCC
AMLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYDPTRDTILNPMDARCPA
EEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCHHHHCCCCCCCCCC
WSIFNDCRTHSEFTAAAAALIPSDGGSSEPFWALAARTLFIEMCIRLMERGQTSNLALSE
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
NLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNTNAQVLRFLPDEGESFSIRDW
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEHHHH
ITRDKKPGSILFVTSNYVDLPMNRALLTLWMDLAINRLMTLPRTRSLRTWFMFDELGALH
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RLPAIENGLQTARAFGGAMILGIHSFEKLVEVYGEQGARNLASLARSKLILATADLDTAE
HCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCHHH
QCARYIGNREVRQMDEAYSYGYNNTRDASTLTPRKQVEPLVIADDITNLPSMHGFVKFPD
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCEEECCC
GFPAARILLEWKDYPQVAQGFVQRPDVGPVRSRRGEEVFEEDGAGEAGGRDGSLQVVEEV
CCCHHHHEEECCCCHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHH
AEITNLARDLAARILSAEVEEEDDAARRAAQRADDRDEQATPGTHSADRAQAARASGEEQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCC
PVSARDRDDRRDGHRHGETSPQVEDQTLIELRHDFSTGREDDGMDMGI
CCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AREDIRTDGRPVPLTHHSARGRVQRNAGNFTRGSQLLTHEMLMWFSGAKLPFILWFFAF
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LAAWFIIMSLKLDEHGFQLVCMKVYATLWNWIDLNPNKRVNVTLPNGDLMRTVMKAVPYI
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHHHHHHHHCCCH
PEVITAWAVAMRGLIGAFLISVFITIPAAIWFVDISHRRGRSILQERHERGAMLVDRSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEECCEE
LVEINEHNRVKFEEEAADLFPGLSSRQVLALPFRARKEAGIHHPYTLAGIPFPHRTEQSH
EEEECCCCCEEEHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCCEEECCCCCCCCCCCCC
AMLIGTTGSGKTTELRSLVTQMRERQDTAVIFDLTGAYVEAFYDPTRDTILNPMDARCPA
EEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCHHHHCCCCCCCCCC
WSIFNDCRTHSEFTAAAAALIPSDGGSSEPFWALAARTLFIEMCIRLMERGQTSNLALSE
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
NLMTADLKRVHRFLANTIADPLTAPEAARMAESIRAVFNTNAQVLRFLPDEGESFSIRDW
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEHHHH
ITRDKKPGSILFVTSNYVDLPMNRALLTLWMDLAINRLMTLPRTRSLRTWFMFDELGALH
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RLPAIENGLQTARAFGGAMILGIHSFEKLVEVYGEQGARNLASLARSKLILATADLDTAE
HCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEECCCCHHH
QCARYIGNREVRQMDEAYSYGYNNTRDASTLTPRKQVEPLVIADDITNLPSMHGFVKFPD
HHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEECCCCCCCCCCCEEECCC
GFPAARILLEWKDYPQVAQGFVQRPDVGPVRSRRGEEVFEEDGAGEAGGRDGSLQVVEEV
CCCHHHHEEECCCCHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHH
AEITNLARDLAARILSAEVEEEDDAARRAAQRADDRDEQATPGTHSADRAQAARASGEEQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCC
PVSARDRDDRRDGHRHGETSPQVEDQTLIELRHDFSTGREDDGMDMGI
CCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]