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The map label for this gene is istB [H]

Identifier: 148550577

GI number: 148550577

Start: 31056

End: 31823

Strand: Reverse

Name: istB [H]

Synonym: Swit_5138

Alternate gene names: 148550577

Gene position: 31823-31056 (Counterclockwise)

Preceding gene: 148550578

Following gene: 148550576

Centisome position: 10.26

GC content: 62.63

Gene sequence:

>768_bases
ATGAGCGATCAGGCCCCGGAGATCCTTCTCGCTCACCATCTCAAGGCGCTCAAGCTGCCTACGTGCCTGCGTGAGCATCA
CAAGCTCGCGCGGCAATGTGCCGCTGAAGGCGTCGATCATATCCGCTTCCTCGCCCGCCTCGTCGAGATGGAAATGATCG
ACAGGGAGCGTCGCATGGTCGAGCGGCGCATCAAGGCCGCGCGCTTCCCCGCCGTCAAAAGCCTCGACAGCTTCGACTTC
GCCGCCATCCCCAGGCTCAACAAGATGCAGGTGCTCGAGATGGCGCGCTGCGAGTGGATCGAGCGGCGTGAGAACGCCAT
CGCTCTGGGGCCATCAGGCACCGGAAAGACGCACGTAGCGTTGGGGCTCGGACTGGCAGCATGCCAGAAAGGACTGTCGG
TGGGCTTCACCACCGCGGCAGCGCTGGTCAGCGAAATGATGGAGGCCCGCGACGAGCGCCGTCTTCTGCGCTTCCAGAAG
CAGATGGCCGGATACAAGCTGCTCATCATCGACGAACTGGGCTTTGTGCCGCTCTCCAAGACCGGCGCCGAACTGTTGTT
CGAGCTGATCTCCCAGCGTTACGAACGCGGCTCCACCTTCATCACCAGCAACCTGCCCTTCGACGAATGGACCGAAACCT
TCGGATCTGAGCGTCTCACAGGCGCGCTCCTCGATCGCCTGACCCATCACGTCAGCATCCTCGAGATGAACGGCGAAAGC
TATCGCCTCGCGCACAGCCGGGCCCGCAAGGCCAAAACCAGACCCTGA

Upstream 100 bases:

>100_bases
GGCTGGACCTGGACGTCTATCCCTTCCTGCCACGCACCACGGTCGAGAAGACCTTTGCCAGAGCCTATCTGAGCCTGCTC
TCCGACCGGCAGGAGGCCGC

Downstream 100 bases:

>100_bases
AAATTACACCAATGCCGGGGGGAGTGGCCCTCGGGCTACGCCCTCACGCCACTCCCCCCGGCATGTAACACGATGGCCTG
GTTTTACGCCGCCGAATGGC

Product: IstB ATP binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMVERRIKAARFPAVKSLDSFDF
AAIPRLNKMQVLEMARCEWIERRENAIALGPSGTGKTHVALGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQK
QMAGYKLLIIDELGFVPLSKTGAELLFELISQRYERGSTFITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGES
YRLAHSRARKAKTRP

Sequences:

>Translated_255_residues
MSDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMVERRIKAARFPAVKSLDSFDF
AAIPRLNKMQVLEMARCEWIERRENAIALGPSGTGKTHVALGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQK
QMAGYKLLIIDELGFVPLSKTGAELLFELISQRYERGSTFITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGES
YRLAHSRARKAKTRP
>Mature_254_residues
SDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMVERRIKAARFPAVKSLDSFDFA
AIPRLNKMQVLEMARCEWIERRENAIALGPSGTGKTHVALGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQKQ
MAGYKLLIIDELGFVPLSKTGAELLFELISQRYERGSTFITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGESY
RLAHSRARKAKTRP

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28897; Mature: 28766

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ERRIKAARFPAVKSLDSFDFAAIPRLNKMQVLEMARCEWIERRENAIALGPSGTGKTHVA
HHHHHHHHCCHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHH
LGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQKQMAGYKLLIIDELGFVPLSK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
TGAELLFELISQRYERGSTFITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGES
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCH
YRLAHSRARKAKTRP
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
ERRIKAARFPAVKSLDSFDFAAIPRLNKMQVLEMARCEWIERRENAIALGPSGTGKTHVA
HHHHHHHHCCHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHH
LGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQKQMAGYKLLIIDELGFVPLSK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCH
TGAELLFELISQRYERGSTFITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGES
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCH
YRLAHSRARKAKTRP
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]