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The map label for this gene is prfC [H]

Identifier: 148550566

GI number: 148550566

Start: 12109

End: 13674

Strand: Direct

Name: prfC [H]

Synonym: Swit_5127

Alternate gene names: 148550566

Gene position: 12109-13674 (Clockwise)

Preceding gene: 148550565

Following gene: 148550569

Centisome position: 3.9

GC content: 64.18

Gene sequence:

>1566_bases
ATGACCATTCCATCCCGCCGCACTTTCGCGATCATTTCCCACCCTGACGCCGGCAAGACCACGCTTACCGAAAAACTGCT
GCTGGAGGGCGGAGCGATCCACCTTGCCGGCGAGGTCAAGGCGCGTGGCGCCAACCGCCGCGCCCGTTCGGACTGGATGA
AGATCGAGCAGCAGCGCGGCATTTCGGTCACCAGTTCGGTGATGACGTTCGAGCGGGACGGCGTCACCTTCAACCTGCTC
GACACGCCGGGGCACGAGGATTTCAGCGAGGATACCTATCGCACGCTGACCGCCGTCGACAGCGCCGTGATGGTGATCGA
CGCGGCCAAGGGCATCGAGCCGCAGACACGCAAGCTGTTCGAGGTATGCCGCCTGCGCAACGTGCCGATCATCACCTTCG
TCAACAAGGTGGACCGGGAGGGGCGCGACCCCTTCGCACTGCTGGACGAAGTGGCCGATGCGCTGGCGCTCGACGTATGC
CCGATGAGCTGGCCGGTCGGCATGGGCGGCACGTTCGAAGGCATCTACGACTTTGCGAAGAACCGGCTGCGGCAGCCCAC
GGGAGCAAGCAAGGAGTTCGAGGGCAAGGAAACGCATGTCTCCGGCCTTGCCGATCCCGCGCTGGCCGACGTGTTGAGCG
AGGATGGCCTGGCGCTGCTGCGCGAGGAGGCGGAACTGGCCCAGGGTGGCTACGCCAGTTTCGATCTTGCCGCCTATCGC
CATGGCGACCTGACCCCGGTCTATTTCGGATCGGCGCTGAAGCTGTTCGGCGTGAACGAACTGATCGACGCGCTCGCCGC
CTATGCGCCGCCGCCGCGCGCGCAACCCGCCGAACCGGCAGCCATCGAACCGGAACAGGGCGAGGTTACCGGCTTCATCT
TCAAGGTGCAGGCGAACATGGACCCGCAGCACCGGGACCGTATCGCATTCATGCGGCTATGTTCCGGCAAATTCCGGCGC
GGCATGAAGCTGACGCCGTCGGGATCGGGAAAGCCGATCGCCGTGCATTCGCCCATCCTGTTCTTCGCGCAGGACCGCGA
GATCGCCGATGAGGCATTTCCCGGCGACATCATCGGCATTCCCAATCACGGCACGCTGCGCGTGGGCGATACGCTGTCGG
AAAAGAACGATGTGCGCTTCACCGGCCTGCCCAATTTCGCGCCGGAAATCCTGCGCCGTGTCGTGTTGAAGGACCCGACC
AAGACCAAGCAGCTGCGCAAGGCGCTGGACGATCTGTCGGAAGAAGGCGTGATCCAGGTTTTCTACCCGGAAATCGGCAG
CCAGTGGGTCGTCGGCGTGGTCGGCCAGCTGCAGCTGGACGTGCTGATTTCCCGGCTGGAGGCGGAATATAAGGTCGGCG
CCATGCTGGAACCTGCGCCGTTCGAGACCGCCCGCTGGATCGGCGGCCCGGATGCCGCGATCAAGGGACTGGTGGAGTTC
AACCGGTCCAACCTGGCGCGCGACCGCGACGGCAATCTGGTGTTCATGGCGAAAAGCGCGTGGGACGTGAACTATCAGCA
GGAACGCAATCCCGACGTGACGTTCAGCGCCACCAAGGAACGTTAG

Upstream 100 bases:

>100_bases
GTCTTGATCCCGGTGACCAGCGTTCCTTCCACATGAACGTGACGCTTCAGGCGGGCTGAAAGCTGGCGTTTATCGCGATT
TGTGCTTAGGGAGCCGCCCC

Downstream 100 bases:

>100_bases
GACTGGGACGCGTCAGGCGCTCACCCAGTCCTTGCGCAGCGACTTTCCGGCGAGCGCCATGAACAGGCCAGCGAGCAGGT
AGAAGCCGAGCGCGGCCACA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRGISVTSSVMTFERDGVTFNLL
DTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTRKLFEVCRLRNVPIITFVNKVDREGRDPFALLDEVADALALDVC
PMSWPVGMGGTFEGIYDFAKNRLRQPTGASKEFEGKETHVSGLADPALADVLSEDGLALLREEAELAQGGYASFDLAAYR
HGDLTPVYFGSALKLFGVNELIDALAAYAPPPRAQPAEPAAIEPEQGEVTGFIFKVQANMDPQHRDRIAFMRLCSGKFRR
GMKLTPSGSGKPIAVHSPILFFAQDREIADEAFPGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVVLKDPT
KTKQLRKALDDLSEEGVIQVFYPEIGSQWVVGVVGQLQLDVLISRLEAEYKVGAMLEPAPFETARWIGGPDAAIKGLVEF
NRSNLARDRDGNLVFMAKSAWDVNYQQERNPDVTFSATKER

Sequences:

>Translated_521_residues
MTIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRGISVTSSVMTFERDGVTFNLL
DTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTRKLFEVCRLRNVPIITFVNKVDREGRDPFALLDEVADALALDVC
PMSWPVGMGGTFEGIYDFAKNRLRQPTGASKEFEGKETHVSGLADPALADVLSEDGLALLREEAELAQGGYASFDLAAYR
HGDLTPVYFGSALKLFGVNELIDALAAYAPPPRAQPAEPAAIEPEQGEVTGFIFKVQANMDPQHRDRIAFMRLCSGKFRR
GMKLTPSGSGKPIAVHSPILFFAQDREIADEAFPGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVVLKDPT
KTKQLRKALDDLSEEGVIQVFYPEIGSQWVVGVVGQLQLDVLISRLEAEYKVGAMLEPAPFETARWIGGPDAAIKGLVEF
NRSNLARDRDGNLVFMAKSAWDVNYQQERNPDVTFSATKER
>Mature_520_residues
TIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRGISVTSSVMTFERDGVTFNLLD
TPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTRKLFEVCRLRNVPIITFVNKVDREGRDPFALLDEVADALALDVCP
MSWPVGMGGTFEGIYDFAKNRLRQPTGASKEFEGKETHVSGLADPALADVLSEDGLALLREEAELAQGGYASFDLAAYRH
GDLTPVYFGSALKLFGVNELIDALAAYAPPPRAQPAEPAAIEPEQGEVTGFIFKVQANMDPQHRDRIAFMRLCSGKFRRG
MKLTPSGSGKPIAVHSPILFFAQDREIADEAFPGDIIGIPNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVVLKDPTK
TKQLRKALDDLSEEGVIQVFYPEIGSQWVVGVVGQLQLDVLISRLEAEYKVGAMLEPAPFETARWIGGPDAAIKGLVEFN
RSNLARDRDGNLVFMAKSAWDVNYQQERNPDVTFSATKER

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.5010351966874, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=26.5225933202358, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI25306283, Length=339, Percent_Identity=30.9734513274336, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=31.6326530612245, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI157426893, Length=142, Percent_Identity=33.8028169014084, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.1167883211679, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.8724832214765, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=118, Percent_Identity=34.7457627118644, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI310110807, Length=118, Percent_Identity=34.7457627118644, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI310123363, Length=118, Percent_Identity=34.7457627118644, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=50.3831417624521, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=27.9352226720648, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=33.5227272727273, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=177, Percent_Identity=31.0734463276836, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=26.369168356998, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=481, Percent_Identity=24.5322245322245, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=33.5766423357664, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506493, Length=163, Percent_Identity=27.6073619631902, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=26.5690376569038, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=517, Percent_Identity=25.1450676982592, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324707, Length=153, Percent_Identity=31.3725490196078, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=153, Percent_Identity=31.3725490196078, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=176, Percent_Identity=26.7045454545455, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=26.1954261954262, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI221458488, Length=525, Percent_Identity=27.047619047619, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=34.1614906832298, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585711, Length=171, Percent_Identity=30.9941520467836, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=171, Percent_Identity=30.9941520467836, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585709, Length=171, Percent_Identity=30.9941520467836, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 57317; Mature: 57186

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRG
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHCC
ISVTSSVMTFERDGVTFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTRKLF
CEEEHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
EVCRLRNVPIITFVNKVDREGRDPFALLDEVADALALDVCPMSWPVGMGGTFEGIYDFAK
HHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
NRLRQPTGASKEFEGKETHVSGLADPALADVLSEDGLALLREEAELAQGGYASFDLAAYR
HHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEE
HGDLTPVYFGSALKLFGVNELIDALAAYAPPPRAQPAEPAAIEPEQGEVTGFIFKVQANM
CCCCCEEECCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
DPQHRDRIAFMRLCSGKFRRGMKLTPSGSGKPIAVHSPILFFAQDREIADEAFPGDIIGI
CHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCEEEEECCCHHHHHCCCCCEEEC
PNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSEEGVIQV
CCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
FYPEIGSQWVVGVVGQLQLDVLISRLEAEYKVGAMLEPAPFETARWIGGPDAAIKGLVEF
EECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCCCHHHHHHHHHH
NRSNLARDRDGNLVFMAKSAWDVNYQQERNPDVTFSATKER
HHHHCCCCCCCCEEEEEECCCCCCCHHCCCCCEEEEECCCC
>Mature Secondary Structure 
TIPSRRTFAIISHPDAGKTTLTEKLLLEGGAIHLAGEVKARGANRRARSDWMKIEQQRG
CCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHCC
ISVTSSVMTFERDGVTFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEPQTRKLF
CEEEHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
EVCRLRNVPIITFVNKVDREGRDPFALLDEVADALALDVCPMSWPVGMGGTFEGIYDFAK
HHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
NRLRQPTGASKEFEGKETHVSGLADPALADVLSEDGLALLREEAELAQGGYASFDLAAYR
HHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEE
HGDLTPVYFGSALKLFGVNELIDALAAYAPPPRAQPAEPAAIEPEQGEVTGFIFKVQANM
CCCCCEEECCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCC
DPQHRDRIAFMRLCSGKFRRGMKLTPSGSGKPIAVHSPILFFAQDREIADEAFPGDIIGI
CHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCEEEEECCCHHHHHCCCCCEEEC
PNHGTLRVGDTLSEKNDVRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSEEGVIQV
CCCCEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
FYPEIGSQWVVGVVGQLQLDVLISRLEAEYKVGAMLEPAPFETARWIGGPDAAIKGLVEF
EECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHCCCCCHHHHHHHHHH
NRSNLARDRDGNLVFMAKSAWDVNYQQERNPDVTFSATKER
HHHHCCCCCCCCEEEEEECCCCCCCHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA