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The map label for this gene is nagB

Identifier: 148380792

GI number: 148380792

Start: 3007452

End: 3008186

Strand: Reverse

Name: nagB

Synonym: CBO2833

Alternate gene names: 148380792

Gene position: 3008186-3007452 (Counterclockwise)

Preceding gene: 148380793

Following gene: 148380791

Centisome position: 77.39

GC content: 28.98

Gene sequence:

>735_bases
ATGAGAATTATAGTAGTGGATAATTATGAAGAAATGAGTAAAAAAGCGGCAGCTATGGTAGCTAGCCAAGTTATTTTAAA
ACCAGATAGTGTTCTTGGATTGGCAACTGGAGATACCCCTATAGGAATGTATAAAGAAATCATAAATATTTATAAAAATC
AAAAGATGGATTTCTCTAAAGCTAGAACCTTTAATTTAGATGAATACTATGGGTTAAACAGAGAAAATCCTCAGAGTTAT
TATTATTACATGATGAATAATTTATTTAATCATGTGAATATTGATAAAAATAATATTAATATTCCTAATGGAATGGCTGA
TAATATAGAAATAGAGTGTAAGGAATATGAAAGAAAAATAGATAAAGCAGGTGGTATTGATTTACAAATTCTTGGTATCG
GTGTCAATGGGCATATTGGGTTTAATGAACCCAATATAAGTTTTGAATCAGAAACTCATCTAGTTAATCTAAATGAAAAA
ACTATAGAGTCTAATTCAAGATTTTTTAGCTCGAAAGAGGAAGTTCCAACAAAAGCTATTAGTGTGGGAATTAAAAGTAT
AATACATTCCAAAAAGATAATCCTATTAGCTTGTGGTAGTGCAAAATCTGACGCTGTTTCTAAAACTATAAATGGTAAAA
TTAACCCTAATATACCTGCATCTATTCTTCAATTACACAGAGATGTTGTTGTTATTATAGATAAGGAAGCTGCTAGCAAG
TTAAATTTAAAGTAG

Upstream 100 bases:

>100_bases
ATGACAATTGTTGATGGAAATATTGTGACTTAGATTATTAGACACAATTAGTTATTGATTTAATAAAAGAGAAATTTAAA
TTTTAATGAGGTGTGTTTTT

Downstream 100 bases:

>100_bases
AGTAGTTTAAAATATAAGTGTTAGGAATTCATATCCTTAAGTTATGAGGAAGAACTGCCTAACACTTTTTTAAATTCATT
AATATGTAAAAAATACAAAA

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRIIVVDNYEEMSKKAAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSKARTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHRDVVVIIDKEAASK
LNLK

Sequences:

>Translated_244_residues
MRIIVVDNYEEMSKKAAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSKARTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHRDVVVIIDKEAASK
LNLK
>Mature_244_residues
MRIIVVDNYEEMSKKAAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSKARTFNLDEYYGLNRENPQSY
YYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKIDKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEK
TIESNSRFFSSKEEVPTKAISVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHRDVVVIIDKEAASK
LNLK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI19923881, Length=250, Percent_Identity=40, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI13027378, Length=250, Percent_Identity=38.4, Blast_Score=193, Evalue=1e-49,
Organism=Escherichia coli, GI1786893, Length=250, Percent_Identity=38, Blast_Score=186, Evalue=8e-49,
Organism=Escherichia coli, GI1789530, Length=229, Percent_Identity=28.82096069869, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI48994958, Length=235, Percent_Identity=26.8085106382979, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17554876, Length=249, Percent_Identity=39.7590361445783, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24581960, Length=250, Percent_Identity=38.8, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19920764, Length=250, Percent_Identity=38.8, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_CLOB1 (A7FX73)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385077.1
- ProteinModelPortal:   A7FX73
- SMR:   A7FX73
- STRING:   A7FX73
- GeneID:   5397700
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2777
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- BioCyc:   CBOT441770:CLB_2777-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 27287; Mature: 27287

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 136-136 ACT_SITE 138-138 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIVVDNYEEMSKKAAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSK
CEEEEECCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHH
ARTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKI
CEEECCHHHCCCCCCCCCCEEHEEHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHH
DKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAI
HHCCCCEEEEEEEEECCEECCCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHCCHHHH
SVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHRDVVVIIDKEAASK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCC
LNLK
CCCC
>Mature Secondary Structure
MRIIVVDNYEEMSKKAAAMVASQVILKPDSVLGLATGDTPIGMYKEIINIYKNQKMDFSK
CEEEEECCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHH
ARTFNLDEYYGLNRENPQSYYYYMMNNLFNHVNIDKNNINIPNGMADNIEIECKEYERKI
CEEECCHHHCCCCCCCCCCEEHEEHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHH
DKAGGIDLQILGIGVNGHIGFNEPNISFESETHLVNLNEKTIESNSRFFSSKEEVPTKAI
HHCCCCEEEEEEEEECCEECCCCCCCCCCCCEEEEECCHHHHCCCCHHCCCCHHCCHHHH
SVGIKSIIHSKKIILLACGSAKSDAVSKTINGKINPNIPASILQLHRDVVVIIDKEAASK
HHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCC
LNLK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA