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The map label for this gene is fliF [H]

Identifier: 148380620

GI number: 148380620

Start: 2812453

End: 2814033

Strand: Reverse

Name: fliF [H]

Synonym: CBO2662

Alternate gene names: 148380620

Gene position: 2814033-2812453 (Counterclockwise)

Preceding gene: 148380621

Following gene: 148380619

Centisome position: 72.4

GC content: 31.37

Gene sequence:

>1581_bases
ATGAATAAGCTAAAGGAGTTTTTTAAAGGGTTAAAAGAAAAGTGGACAGGGTTTAGTAAAGTAAAGAAAATAGCTTTTTC
TATAATTTTTTTAGGAATTATAACATCTATTATTGCATTATCTCTTTATTTTGGAAAAACTAAGTATGCAGTTTTATTTT
CCAATATGGATTCTAATGATTCCGGCACGGTACTTCAAAAATTAAAGGATAAAAAAGTTGAAGCTAAGGTAGAGGGAAAT
AATATATTAGTACCTAAAGATAAGGTTGATGAACTAAGGATGCAAATGTTATCTGAAGTTCCATTAACTAATGGTAGTCA
AGGATTTGAAATATTAGATAAAAGTCAGTTTGGAGAAACAGATCAGGAAATGAAAATAAACTATCAAAGAGCCCTTCAAG
GAGAATTAGAACGAACTATAAAAGGATTTCCGCAAGTGGATAATACCAGAGTTCATTTAGTTCTTCCTGAAGAAACTGCC
TTTGTAAAGGAAACACAGCCAGGTAGAGCCTCTGTTACTTTAAACTTAAAACAAGGACAAACACTTAGTAAGGAGCAAGT
TAAATCTATAGTAGCTTTAGTAAGTGGAAGTGTGAAAAATATTCCGAAAGAAAATGTAGAAGTAATTGATAACAACATGA
ATCTTCTTACAAAGAATTTATTTGATGCTTCAGGAAGTTTAGAAGAGGCTACAACTTCAGCAGAAAAGCAGCAACAACTT
CAAAAAAATTATGAGAAAGATCTTCAAAATAGACTAGTTAGTATGTTGGAAGCTGTATATGGAAAGGACAAGGTTAAAGT
TAATATTAACACAGATTTAGATTTTGATGCAGTTAAAACTAATTCTGTAACCTATGATCCTAAAAATGTAGTAGTTAGTG
AACATAGCATTAAAGAGAAAAATCAGAATAATGCAGGTGGAAATAATACAAATGGTAGTGTAGTAGATAATAATATGGTA
AATAGAACTACCCAAAATAATAATGGTAGTGAGACATCTTCTAGAGATGAAAATACTAAAAATTATGAAATATCAAAAAC
TCAGCAGGATAGTATAAAGGCTCCAGGTTCTGTAAAAAGGTTAACAGCGTCTATTGTGTTAGATGGAAATATAGATGAAG
AAACTAGAAGTGCTGTTAGAAATTTAGCTGTATCTGCTATAGGCTATGATGAAAAAAGAGGAGATACCATAAACGTAGAG
GGTCTTCCATTTGATACAGCGGCTAAGGATAAAGTGAAAAAAGATTTAGAAGATATGCAAAAAGCTGAAAAAACAAAAGA
AAGAATAAAATTGTTTACTGCTATAGGGCTAGGGGTACTACTGTTATTGGGAGCAATAATATTCTTTATTATTAAGAAAC
GAAACGAAGATGAAGAGTATGAAGAAGATGAAGAAGGTCTTGATATTTTAATAGATGATAACGACAGTGAAACTAAACAG
GTTCCTAAGTTTAAACCTATAGATTTAGAAGCACAGGATGAAAAGACACATGTTGAAAATGAAATAAGAAAATATGCTAA
GGACAAACCTGATCAAGTGGCGGAAATAATTAAATCATGGCTAGCAGAGGATGAGAGGTGA

Upstream 100 bases:

>100_bases
AGCTAAAATGTCCATGGAGCTAGCAGTCCAGGTTAGAAACAAGCTAGTAGAAGCTTATCAAGAATTAAGTAGAATGCAAT
TATAGTAAGGGGAGCTTTCG

Downstream 100 bases:

>100_bases
TAATATATGGCTAAAGATGATAAATTAACAGGAGTTCAAAAAGCAGCAATACTATTTATTACTTTAGGTCCTGAGGCCTC
TGCTGGTATAATAAAAAAAT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQTLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKAEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNEDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLEAQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER

Sequences:

>Translated_526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQTLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKAEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNEDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLEAQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
>Mature_526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQTLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKAEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNEDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLEAQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=423, Percent_Identity=29.7872340425532, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59045; Mature: 59045

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSND
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
SGTVLQKLKDKKVEAKVEGNNILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGET
CHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCHHHCCCC
DQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETAFVKETQPGRASVTLNLKQGQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCC
TLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
CCCHHHHHHHHHHHHCCHHCCCCCCHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEK
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEECCCCEEECCHHHHHC
NQNNAGGNNTNGSVVDNNMVNRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKR
CCCCCCCCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCCCHHHH
LTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVEGLPFDTAAKDKVKKDLEDMQ
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KAEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNEDEEYEEDEEGLDILIDDNDSETKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCCCHHHC
VPKFKPIDLEAQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSND
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
SGTVLQKLKDKKVEAKVEGNNILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGET
CHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCHHHCCCC
DQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETAFVKETQPGRASVTLNLKQGQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCC
TLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
CCCHHHHHHHHHHHHCCHHCCCCCCHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEK
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEECCCCEEECCHHHHHC
NQNNAGGNNTNGSVVDNNMVNRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKR
CCCCCCCCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCCCHHHH
LTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVEGLPFDTAAKDKVKKDLEDMQ
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KAEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNEDEEYEEDEEGLDILIDDNDSETKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEECCCCCHHHC
VPKFKPIDLEAQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]