The gene/protein map for NC_009495 is currently unavailable.
Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is ldh

Identifier: 148379488

GI number: 148379488

Start: 1653007

End: 1653963

Strand: Reverse

Name: ldh

Synonym: CBO1519

Alternate gene names: 148379488

Gene position: 1653963-1653007 (Counterclockwise)

Preceding gene: 148379490

Following gene: 148379480

Centisome position: 42.55

GC content: 29.89

Gene sequence:

>957_bases
ATGATAAAAAAAAGAAATACTACTAAAATATCAGTTATAGGAGCAGGTTCAGTAGGTGCAACCACTGCTTATGCATTAAT
GCTATCAGGTGTTGCAACAGAAATAGTATTAGTAGATGTTAATAAATCAAAAACTGAGGGAGAGGCTATGGATTTATCCC
ATGGAGCAGATTTCGTAAAGCCTGTAAATATACTAAGTGGAGATTATAAAGATACTGAAGGTTCAGATATTGTTGTAATT
ACAGCTGGCGCAGCACAAAAAGTAGGTGAAACAAGACTTCAACTTATAAATAAAAATATTAATATATTTAAATCAATAAT
TCCACAAGTAGTAAAATATAATAAAGATGCTATTTTATTAGTAGTTTCAAATCCTGTAGATGTATTAAGTTATGTAACCT
ATAAACTATCAGGCTTCCCTAAAGAAAGAGTTATAGGTTCTGGTACGGTTTTAGACACATCAAGACTTAAACACGAGATA
GGGAAAAGATATAAAATCGACCCAAGAAACGTAAATACTTATATAATGGGAGAACACGGTGATTCAGAAATCGCTACTTG
GAGTGTAACTAATATTCAAAACATTAAAATTGATGAATATGCTAATAAAGAAAATCTTGAGTATAATGATAACTTTAGAA
AAGAAGTCTATGAAAATGTTAAAAATGCGGCTTACGAAGTTATAAATAGAAAAGGTGCTACTTTCTATGCTATAGCTTTA
GCGGTAACACGTATTGTAAAAGCTATATTAGGGGATGAAAAAACTATATTACCAGTTTCTACTTTAGTAGAAAATTACTA
TGGAATTAAAGATGTTTACCTAGGAATGCCATGTATTGTAGGTGGATCAGGTATTGAAAAAGCATTAAGTATAGATTTAA
ATAAAACAGAAGCATCTAAATTAGTTAAATCTGCTGAAACTTTAAAAAATACTTTAAATAATGCATCTTGTCTATAG

Upstream 100 bases:

>100_bases
CATTGGTAATACCACTAAACCAAAATACCAAAATACCAAAATACCAATAATCTACTGAATTAATTTATATTAAGTTAAAG
TTTTTAGGGGGTAATTTAAA

Downstream 100 bases:

>100_bases
TTTTTATATAAGATAAACATTACTATCAAAATTACCCTATAGATAGACTTTAATATGTGCCCTTTCCAATAACAGATTTT
TATTTTTTAATCTGTCTACC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIKKRNTTKISVIGAGSVGATTAYALMLSGVATEIVLVDVNKSKTEGEAMDLSHGADFVKPVNILSGDYKDTEGSDIVVI
TAGAAQKVGETRLQLINKNINIFKSIIPQVVKYNKDAILLVVSNPVDVLSYVTYKLSGFPKERVIGSGTVLDTSRLKHEI
GKRYKIDPRNVNTYIMGEHGDSEIATWSVTNIQNIKIDEYANKENLEYNDNFRKEVYENVKNAAYEVINRKGATFYAIAL
AVTRIVKAILGDEKTILPVSTLVENYYGIKDVYLGMPCIVGGSGIEKALSIDLNKTEASKLVKSAETLKNTLNNASCL

Sequences:

>Translated_318_residues
MIKKRNTTKISVIGAGSVGATTAYALMLSGVATEIVLVDVNKSKTEGEAMDLSHGADFVKPVNILSGDYKDTEGSDIVVI
TAGAAQKVGETRLQLINKNINIFKSIIPQVVKYNKDAILLVVSNPVDVLSYVTYKLSGFPKERVIGSGTVLDTSRLKHEI
GKRYKIDPRNVNTYIMGEHGDSEIATWSVTNIQNIKIDEYANKENLEYNDNFRKEVYENVKNAAYEVINRKGATFYAIAL
AVTRIVKAILGDEKTILPVSTLVENYYGIKDVYLGMPCIVGGSGIEKALSIDLNKTEASKLVKSAETLKNTLNNASCL
>Mature_318_residues
MIKKRNTTKISVIGAGSVGATTAYALMLSGVATEIVLVDVNKSKTEGEAMDLSHGADFVKPVNILSGDYKDTEGSDIVVI
TAGAAQKVGETRLQLINKNINIFKSIIPQVVKYNKDAILLVVSNPVDVLSYVTYKLSGFPKERVIGSGTVLDTSRLKHEI
GKRYKIDPRNVNTYIMGEHGDSEIATWSVTNIQNIKIDEYANKENLEYNDNFRKEVYENVKNAAYEVINRKGATFYAIAL
AVTRIVKAILGDEKTILPVSTLVENYYGIKDVYLGMPCIVGGSGIEKALSIDLNKTEASKLVKSAETLKNTLNNASCL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=43.5897435897436, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=43.5897435897436, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=44.0397350993377, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=44.0397350993377, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI47059044, Length=302, Percent_Identity=44.0397350993377, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI221136809, Length=302, Percent_Identity=44.0397350993377, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI15082234, Length=313, Percent_Identity=42.8115015974441, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=42.3452768729642, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=42.3452768729642, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI260099727, Length=223, Percent_Identity=43.9461883408072, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=47, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI207028494, Length=311, Percent_Identity=31.5112540192926, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI103472011, Length=308, Percent_Identity=29.2207792207792, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI103472015, Length=186, Percent_Identity=32.258064516129, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI21735621, Length=314, Percent_Identity=26.4331210191083, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789632, Length=324, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535107, Length=296, Percent_Identity=42.2297297297297, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17554310, Length=300, Percent_Identity=25.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322765, Length=304, Percent_Identity=26.9736842105263, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=40.1315789473684, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI45550422, Length=322, Percent_Identity=33.5403726708075, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24647881, Length=318, Percent_Identity=25.7861635220126, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24663599, Length=324, Percent_Identity=25, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_CLOB1 (A7FU32)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383865.1
- ProteinModelPortal:   A7FU32
- SMR:   A7FU32
- STRING:   A7FU32
- GeneID:   5397512
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1540
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   CLSK971982
- BioCyc:   CBOT441770:CLB_1540-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34734; Mature: 34734

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MIKKRNTTKISVIGAGSVGATTAYALMLSGVATEIVLVDVNKSKTEGEAMDLSHGADFVK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCCCCHHH
PVNILSGDYKDTEGSDIVVITAGAAQKVGETRLQLINKNINIFKSIIPQVVKYNKDAILL
HHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEE
VVSNPVDVLSYVTYKLSGFPKERVIGSGTVLDTSRLKHEIGKRYKIDPRNVNTYIMGEHG
EECCCHHHHHHHHHHHCCCCHHHCCCCCCEEEHHHHHHHHCCCEECCCCCCCEEEEECCC
DSEIATWSVTNIQNIKIDEYANKENLEYNDNFRKEVYENVKNAAYEVINRKGATFYAIAL
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVTRIVKAILGDEKTILPVSTLVENYYGIKDVYLGMPCIVGGSGIEKALSIDLNKTEASK
HHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHCCCCEEECCCCCCHHEEECCCHHHHHH
LVKSAETLKNTLNNASCL
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKKRNTTKISVIGAGSVGATTAYALMLSGVATEIVLVDVNKSKTEGEAMDLSHGADFVK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCCCCHHH
PVNILSGDYKDTEGSDIVVITAGAAQKVGETRLQLINKNINIFKSIIPQVVKYNKDAILL
HHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEE
VVSNPVDVLSYVTYKLSGFPKERVIGSGTVLDTSRLKHEIGKRYKIDPRNVNTYIMGEHG
EECCCHHHHHHHHHHHCCCCHHHCCCCCCEEEHHHHHHHHCCCEECCCCCCCEEEEECCC
DSEIATWSVTNIQNIKIDEYANKENLEYNDNFRKEVYENVKNAAYEVINRKGATFYAIAL
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AVTRIVKAILGDEKTILPVSTLVENYYGIKDVYLGMPCIVGGSGIEKALSIDLNKTEASK
HHHHHHHHHHCCCCEEEHHHHHHHHHCCHHHHHCCCCEEECCCCCCHHEEECCCHHHHHH
LVKSAETLKNTLNNASCL
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA