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The map label for this gene is topB [H]

Identifier: 148379477

GI number: 148379477

Start: 1638024

End: 1639883

Strand: Direct

Name: topB [H]

Synonym: CBO1506

Alternate gene names: 148379477

Gene position: 1638024-1639883 (Clockwise)

Preceding gene: 148379475

Following gene: 148379478

Centisome position: 42.14

GC content: 26.83

Gene sequence:

>1860_bases
ATGAGCAAATTATTATTAGCAGAAAAGCCCTCTGTAGCTAGAAATATAGGAGAGGCACTAGGATGTAAAACTAGAAAAAA
TGGATATTTAGAAGGGAATGGATATATAGTTACCTGGGCCTTTGGTCATTTATTAACCTTATATGATTGTAAAGATTACG
ATCATAAATTAGCACTTTGGAGTTTCGAAAATTTTCCATATATACCTACAGAATTTAAATATAAAGTAAAAAATGACGGT
AAAAATAGAAATGTAGTAGATGCAGGAGCAAAAAAACAACTTGAAATAATAAAGGAACTTATAAATAGAGGGGATGTAGA
AGAGATAATATCCGCTACTGACTATGATAGAGAAGGAGAACTTATAGCTCTTCTAATATTTAATTATTTAAAAGCAAATA
AACCTATATATAGAATACTTATAAATGAATGGACACCTACAGAAATAAAGAAAGGTCTTAAAGATCTTAAAAAGAATGAA
GAAATGATAAGCTTACAGGATGCAGGAGTAAGTAGACAATTAGCAGATTGGGTTATAGGAATAAATTTCACATCTATAGC
CACCATGAAATATACAAGAGGAAAGGGAAATCTTCTTAATATAGGTAGAGTATTAATGCCAACTTTAAAGATTATATATG
ATAGAGAAATAGAAATTAAAAATTTTAAGGTAGAAGAGTTTTATGAACTAGAAGCCACTTTTAAAAATAAAAAGGGAGAA
TATAAAGGTAAATTTTTTTATGAGAAAAAAGAAAAATTTCCAAATAAAAAAATTATGGAAAAGCTTAAAGCTGAAATAAA
GGACAAAAGTGGATTAGTAACAGAAAAAACTATAGAGATTAAAAAGGAAAATCCACCAAGTTTATTTAATTTAAGTAATC
TTCAAGGATATATTACAAGTAAATATAAAGGTTTTACTGCAGATAAAGTTTTAAAGGTAGCTCAAAGTCTTTATGAAAAA
AAACATATAACTTATCCAAGAACGGAAAGTACGGCTTTAGAAGAAAGTATTAAGGATAAAGCTAAAAAAGTATTAGAGGT
TTTGAAAAATGATTTACCTTTTAAAGATGAAATAATCTTTAATACTTCAAAAAAGGTATTTAACAATGCTAAGGTAGAAA
GCCACAGTGCTATAATGCCAACCTACATAATACCTAAAAATTTAACTCCAGATGAAAGATTAGTATATGATGCAATTAAA
AATAGATTTATATCTCAATTTATGAAAGAAGCACAATATGAAAATACAGAGATAGTAACTACTGTACCAGGAGAAAATTA
TGAAAGATTATTTAAAACAAAAGGGAAAATACTTAAGTGTAAAGGATGGCTAAAATTATATAAAGAAAAGAAGAAAGATG
AACTTTTACCACCTATAGAAAAAGATGATGAAGTAGAAATAAAAAGTTTAAAAATAATATCAAAAAAGACTAAACCACCT
GCTCATCATACAGAAAAGACTTTGCTTAAGGCTATGGAAACTTGTGGCAAAAACACAAAACAAAGTGAAGATAATGAAGA
GGAATCAAATATATTATATGGATATTCTATAGGAACAGCAGCCACTAGAGCAGAAACTATAAATAAGCTAAAATATGCAG
GCTATATAACTCCTAAAGGAAAATCCTTATTAATAACAGATAAAGGTACTAAACTAATAGAGACCTTTCCTATAAGAGAA
TTATTAGATACAGATTATACAGGAAAGCTAGAAAAAAAACTATATGATATGGAAAAGGGAAAATTCAAAAAAGAAGATTT
TTTAAAAGAAATATTTAATTTTACTGTAAATGGAGTAAATAAAATAAAAAATATAAGATCAAATGTAATTTGTGATACAA
GAGTTAAAAAAGATAAATAG

Upstream 100 bases:

>100_bases
GAATGAAATATACCCTATTTTTACAGAATAATTCTACTAAAATATGATATAATCATTAAATGAATAATTAAAACGAATAA
ATAATAGTGATGGTGAGAAA

Downstream 100 bases:

>100_bases
CTCGATAATTAAAAAGGAGGGTTTTAATGTTAGAGGATAAATTTTTAACTAAAATAGAAGAGCAAGAAAAAAACGTTAAA
GAATATAGTAATACCTATAG

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSKLLLAEKPSVARNIGEALGCKTRKNGYLEGNGYIVTWAFGHLLTLYDCKDYDHKLALWSFENFPYIPTEFKYKVKNDG
KNRNVVDAGAKKQLEIIKELINRGDVEEIISATDYDREGELIALLIFNYLKANKPIYRILINEWTPTEIKKGLKDLKKNE
EMISLQDAGVSRQLADWVIGINFTSIATMKYTRGKGNLLNIGRVLMPTLKIIYDREIEIKNFKVEEFYELEATFKNKKGE
YKGKFFYEKKEKFPNKKIMEKLKAEIKDKSGLVTEKTIEIKKENPPSLFNLSNLQGYITSKYKGFTADKVLKVAQSLYEK
KHITYPRTESTALEESIKDKAKKVLEVLKNDLPFKDEIIFNTSKKVFNNAKVESHSAIMPTYIIPKNLTPDERLVYDAIK
NRFISQFMKEAQYENTEIVTTVPGENYERLFKTKGKILKCKGWLKLYKEKKKDELLPPIEKDDEVEIKSLKIISKKTKPP
AHHTEKTLLKAMETCGKNTKQSEDNEEESNILYGYSIGTAATRAETINKLKYAGYITPKGKSLLITDKGTKLIETFPIRE
LLDTDYTGKLEKKLYDMEKGKFKKEDFLKEIFNFTVNGVNKIKNIRSNVICDTRVKKDK

Sequences:

>Translated_619_residues
MSKLLLAEKPSVARNIGEALGCKTRKNGYLEGNGYIVTWAFGHLLTLYDCKDYDHKLALWSFENFPYIPTEFKYKVKNDG
KNRNVVDAGAKKQLEIIKELINRGDVEEIISATDYDREGELIALLIFNYLKANKPIYRILINEWTPTEIKKGLKDLKKNE
EMISLQDAGVSRQLADWVIGINFTSIATMKYTRGKGNLLNIGRVLMPTLKIIYDREIEIKNFKVEEFYELEATFKNKKGE
YKGKFFYEKKEKFPNKKIMEKLKAEIKDKSGLVTEKTIEIKKENPPSLFNLSNLQGYITSKYKGFTADKVLKVAQSLYEK
KHITYPRTESTALEESIKDKAKKVLEVLKNDLPFKDEIIFNTSKKVFNNAKVESHSAIMPTYIIPKNLTPDERLVYDAIK
NRFISQFMKEAQYENTEIVTTVPGENYERLFKTKGKILKCKGWLKLYKEKKKDELLPPIEKDDEVEIKSLKIISKKTKPP
AHHTEKTLLKAMETCGKNTKQSEDNEEESNILYGYSIGTAATRAETINKLKYAGYITPKGKSLLITDKGTKLIETFPIRE
LLDTDYTGKLEKKLYDMEKGKFKKEDFLKEIFNFTVNGVNKIKNIRSNVICDTRVKKDK
>Mature_618_residues
SKLLLAEKPSVARNIGEALGCKTRKNGYLEGNGYIVTWAFGHLLTLYDCKDYDHKLALWSFENFPYIPTEFKYKVKNDGK
NRNVVDAGAKKQLEIIKELINRGDVEEIISATDYDREGELIALLIFNYLKANKPIYRILINEWTPTEIKKGLKDLKKNEE
MISLQDAGVSRQLADWVIGINFTSIATMKYTRGKGNLLNIGRVLMPTLKIIYDREIEIKNFKVEEFYELEATFKNKKGEY
KGKFFYEKKEKFPNKKIMEKLKAEIKDKSGLVTEKTIEIKKENPPSLFNLSNLQGYITSKYKGFTADKVLKVAQSLYEKK
HITYPRTESTALEESIKDKAKKVLEVLKNDLPFKDEIIFNTSKKVFNNAKVESHSAIMPTYIIPKNLTPDERLVYDAIKN
RFISQFMKEAQYENTEIVTTVPGENYERLFKTKGKILKCKGWLKLYKEKKKDELLPPIEKDDEVEIKSLKIISKKTKPPA
HHTEKTLLKAMETCGKNTKQSEDNEEESNILYGYSIGTAATRAETINKLKYAGYITPKGKSLLITDKGTKLIETFPIREL
LDTDYTGKLEKKLYDMEKGKFKKEDFLKEIFNFTVNGVNKIKNIRSNVICDTRVKKDK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=498, Percent_Identity=25.5020080321285, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI4507635, Length=656, Percent_Identity=24.5426829268293, Blast_Score=103, Evalue=7e-22,
Organism=Escherichia coli, GI1788061, Length=630, Percent_Identity=29.0476190476191, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI1787529, Length=606, Percent_Identity=26.8976897689769, Blast_Score=146, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=24.9201277955272, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=639, Percent_Identity=24.4131455399061, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=672, Percent_Identity=23.2142857142857, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640096, Length=648, Percent_Identity=23.9197530864198, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585251, Length=499, Percent_Identity=25.250501002004, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 71501; Mature: 71370

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLLAEKPSVARNIGEALGCKTRKNGYLEGNGYIVTWAFGHLLTLYDCKDYDHKLALW
CCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEHHHEEEEEECCCCCCEEEEE
SFENFPYIPTEFKYKVKNDGKNRNVVDAGAKKQLEIIKELINRGDVEEIISATDYDREGE
EECCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
LIALLIFNYLKANKPIYRILINEWTPTEIKKGLKDLKKNEEMISLQDAGVSRQLADWVIG
EEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHCCCHHHHHHHEEE
INFTSIATMKYTRGKGNLLNIGRVLMPTLKIIYDREIEIKNFKVEEFYELEATFKNKKGE
CCHHEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCC
YKGKFFYEKKEKFPNKKIMEKLKAEIKDKSGLVTEKTIEIKKENPPSLFNLSNLQGYITS
CCCCEEEEHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECCCCCHHEEC
KYKGFTADKVLKVAQSLYEKKHITYPRTESTALEESIKDKAKKVLEVLKNDLPFKDEIIF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NTSKKVFNNAKVESHSAIMPTYIIPKNLTPDERLVYDAIKNRFISQFMKEAQYENTEIVT
CCCHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TVPGENYERLFKTKGKILKCKGWLKLYKEKKKDELLPPIEKDDEVEIKSLKIISKKTKPP
ECCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEHHEEEEHHCCCCC
AHHTEKTLLKAMETCGKNTKQSEDNEEESNILYGYSIGTAATRAETINKLKYAGYITPKG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEECCCC
KSLLITDKGTKLIETFPIRELLDTDYTGKLEKKLYDMEKGKFKKEDFLKEIFNFTVNGVN
CEEEEECCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KIKNIRSNVICDTRVKKDK
HHHHHHHCCEECCCCCCCC
>Mature Secondary Structure 
SKLLLAEKPSVARNIGEALGCKTRKNGYLEGNGYIVTWAFGHLLTLYDCKDYDHKLALW
CCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEEEEHHHEEEEEECCCCCCEEEEE
SFENFPYIPTEFKYKVKNDGKNRNVVDAGAKKQLEIIKELINRGDVEEIISATDYDREGE
EECCCCCCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
LIALLIFNYLKANKPIYRILINEWTPTEIKKGLKDLKKNEEMISLQDAGVSRQLADWVIG
EEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEHHCCCHHHHHHHEEE
INFTSIATMKYTRGKGNLLNIGRVLMPTLKIIYDREIEIKNFKVEEFYELEATFKNKKGE
CCHHEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCC
YKGKFFYEKKEKFPNKKIMEKLKAEIKDKSGLVTEKTIEIKKENPPSLFNLSNLQGYITS
CCCCEEEEHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEECCCCCHHEEC
KYKGFTADKVLKVAQSLYEKKHITYPRTESTALEESIKDKAKKVLEVLKNDLPFKDEIIF
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NTSKKVFNNAKVESHSAIMPTYIIPKNLTPDERLVYDAIKNRFISQFMKEAQYENTEIVT
CCCHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TVPGENYERLFKTKGKILKCKGWLKLYKEKKKDELLPPIEKDDEVEIKSLKIISKKTKPP
ECCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEHHEEEEHHCCCCC
AHHTEKTLLKAMETCGKNTKQSEDNEEESNILYGYSIGTAATRAETINKLKYAGYITPKG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEECCCC
KSLLITDKGTKLIETFPIRELLDTDYTGKLEKKLYDMEKGKFKKEDFLKEIFNFTVNGVN
CEEEEECCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KIKNIRSNVICDTRVKKDK
HHHHHHHCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA