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The map label for this gene is mleN [H]

Identifier: 148379461

GI number: 148379461

Start: 1620788

End: 1622185

Strand: Direct

Name: mleN [H]

Synonym: CBO1490

Alternate gene names: 148379461

Gene position: 1620788-1622185 (Clockwise)

Preceding gene: 148379460

Following gene: 148379462

Centisome position: 41.7

GC content: 32.76

Gene sequence:

>1398_bases
ATGGGAAATACCAATGAAAATAAAGGAAATGGAAAGGCTCTTATACCTTTTGCTATTTTTGTGTTAGTGTATTTATGCTC
AGGAATTATATTATCTATTATGGGAGTAGAAATGGCATTCTATCAATTCCCAGCTCCAATTGCAGTAGTAATAGGAATAA
TATTTGCATTTATTCTAATACAAGGAAGTTTAGATGAAAAAATGGACAGCTTTGTTAAAGGCTGTGGGGACGAGAATATA
ATAATAATGTGTATTATATATCTTTTAGCTGGTGGATTTTCATCAGTTTCTAAAGCAATGGGTGGAGCTGATGCTACAGT
AAATTTAGGATTAACTTTAATACCACCTCAATTTATTGTAGTAGGTATATTTTTAATATCTGCATTTATTTCTATTGCAA
CAGGAACATCAGTAGGAACTGTTGTTGCAGTAGGTCCTATAGCTGCTCAGTTAGTAGCAAAAGCAGGATTAAATATGCCC
TTGGCACTAGGAGCATTAGTTGGAGGAGCTTTATATGGAGATAATTTATCAATTATATCCGATACAACTATAGCTGCTAC
AAGGACCCAAGGGGTAGATATGAAAGATAAATTTAGAGTTAACTTTGGCTTTGCTACGGTAGCAGCTTTAGTTACAATAG
CTTTTTTATTTATATTTGGAAAGCCAGTAACTACACCAAAGATGGTAAGTCATTCATTTAATTTAATAAAAATAATTCCT
TATATGTTTGTTTTAATAGCAGCCTTAGCTGGGAAAAATGTATTCGCAGTATTAATGGGTGGAATAATACTCTCTGGAGC
TATTGGGATTGGTTATGGAGACTTCACAATTTTAGGATTTGCAAAGGAAGTTTATGGAGGATTTACAGGGATGTTTGATA
TTTTTTCATTATCACTGTTAACTGGAGGACTTGCTAATATGGTATCTAAAGGTGGTGGTCTTGACTGGCTTTTATACAAG
ATAAATAAAATGATAAAAGGTAAAAAATCTGCTGAATTAGGAATAAGTTCACTTGTAGCATTAACAGATGCAGCTACAGC
AAATAATACGGTTTCTATAATCGTTTGTGGTGAATTGTCAAAGGAAATATCAAAGGAATATAAAATAGATCCAAGAAAAA
CGGCTTCTCTTTTAAGTACATTTTCAATGGCAATGCAAGGAATAATACCATATGGAGCTCAATTATTGATAGCAGGTAGT
TTTACTAAAGGCTCAGTTAGCCCAGTTCAAATTGTACCTTATATGTGGTATCCATTTATATTAGCTATATTTGGTATAAT
TTTCATATTAACTCCTTTTGGAAATGGTTATTTAAAGAAACATCCTTGGGATTTTGAAAATAGTAGACCTGCGGTAAAGG
GGAGTTTTGAAAATAGTAAGCCTGTAGAAAAGGCATAA

Upstream 100 bases:

>100_bases
TATAGATCTATAATAAAGAATTTAGATCTATAATAAAGAATTTAGATCTATAATAAAGAATTTAGAAAATAACTTTAAAT
TAAGATGAGGAGAGTTTTAA

Downstream 100 bases:

>100_bases
ATTGATTTAAAGGACAAACTCATGGATAAATTTTTATGTGTTTGTCCTCATACCCTTATTTTTCATATGAATATTTTATA
ATTGTAGTTTTTATTTCAGC

Product: Na+/H+ antiporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGNTNENKGNGKALIPFAIFVLVYLCSGIILSIMGVEMAFYQFPAPIAVVIGIIFAFILIQGSLDEKMDSFVKGCGDENI
IIMCIIYLLAGGFSSVSKAMGGADATVNLGLTLIPPQFIVVGIFLISAFISIATGTSVGTVVAVGPIAAQLVAKAGLNMP
LALGALVGGALYGDNLSIISDTTIAATRTQGVDMKDKFRVNFGFATVAALVTIAFLFIFGKPVTTPKMVSHSFNLIKIIP
YMFVLIAALAGKNVFAVLMGGIILSGAIGIGYGDFTILGFAKEVYGGFTGMFDIFSLSLLTGGLANMVSKGGGLDWLLYK
INKMIKGKKSAELGISSLVALTDAATANNTVSIIVCGELSKEISKEYKIDPRKTASLLSTFSMAMQGIIPYGAQLLIAGS
FTKGSVSPVQIVPYMWYPFILAIFGIIFILTPFGNGYLKKHPWDFENSRPAVKGSFENSKPVEKA

Sequences:

>Translated_465_residues
MGNTNENKGNGKALIPFAIFVLVYLCSGIILSIMGVEMAFYQFPAPIAVVIGIIFAFILIQGSLDEKMDSFVKGCGDENI
IIMCIIYLLAGGFSSVSKAMGGADATVNLGLTLIPPQFIVVGIFLISAFISIATGTSVGTVVAVGPIAAQLVAKAGLNMP
LALGALVGGALYGDNLSIISDTTIAATRTQGVDMKDKFRVNFGFATVAALVTIAFLFIFGKPVTTPKMVSHSFNLIKIIP
YMFVLIAALAGKNVFAVLMGGIILSGAIGIGYGDFTILGFAKEVYGGFTGMFDIFSLSLLTGGLANMVSKGGGLDWLLYK
INKMIKGKKSAELGISSLVALTDAATANNTVSIIVCGELSKEISKEYKIDPRKTASLLSTFSMAMQGIIPYGAQLLIAGS
FTKGSVSPVQIVPYMWYPFILAIFGIIFILTPFGNGYLKKHPWDFENSRPAVKGSFENSKPVEKA
>Mature_464_residues
GNTNENKGNGKALIPFAIFVLVYLCSGIILSIMGVEMAFYQFPAPIAVVIGIIFAFILIQGSLDEKMDSFVKGCGDENII
IMCIIYLLAGGFSSVSKAMGGADATVNLGLTLIPPQFIVVGIFLISAFISIATGTSVGTVVAVGPIAAQLVAKAGLNMPL
ALGALVGGALYGDNLSIISDTTIAATRTQGVDMKDKFRVNFGFATVAALVTIAFLFIFGKPVTTPKMVSHSFNLIKIIPY
MFVLIAALAGKNVFAVLMGGIILSGAIGIGYGDFTILGFAKEVYGGFTGMFDIFSLSLLTGGLANMVSKGGGLDWLLYKI
NKMIKGKKSAELGISSLVALTDAATANNTVSIIVCGELSKEISKEYKIDPRKTASLLSTFSMAMQGIIPYGAQLLIAGSF
TKGSVSPVQIVPYMWYPFILAIFGIIFILTPFGNGYLKKHPWDFENSRPAVKGSFENSKPVEKA

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 49094; Mature: 48963

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTNENKGNGKALIPFAIFVLVYLCSGIILSIMGVEMAFYQFPAPIAVVIGIIFAFILI
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QGSLDEKMDSFVKGCGDENIIIMCIIYLLAGGFSSVSKAMGGADATVNLGLTLIPPQFIV
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCEEECCHHHHH
VGIFLISAFISIATGTSVGTVVAVGPIAAQLVAKAGLNMPLALGALVGGALYGDNLSIIS
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
DTTIAATRTQGVDMKDKFRVNFGFATVAALVTIAFLFIFGKPVTTPKMVSHSFNLIKIIP
CCEEEEECCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
YMFVLIAALAGKNVFAVLMGGIILSGAIGIGYGDFTILGFAKEVYGGFTGMFDIFSLSLL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
TGGLANMVSKGGGLDWLLYKINKMIKGKKSAELGISSLVALTDAATANNTVSIIVCGELS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHH
KEISKEYKIDPRKTASLLSTFSMAMQGIIPYGAQLLIAGSFTKGSVSPVQIVPYMWYPFI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHH
LAIFGIIFILTPFGNGYLKKHPWDFENSRPAVKGSFENSKPVEKA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
GNTNENKGNGKALIPFAIFVLVYLCSGIILSIMGVEMAFYQFPAPIAVVIGIIFAFILI
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QGSLDEKMDSFVKGCGDENIIIMCIIYLLAGGFSSVSKAMGGADATVNLGLTLIPPQFIV
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCEEECCHHHHH
VGIFLISAFISIATGTSVGTVVAVGPIAAQLVAKAGLNMPLALGALVGGALYGDNLSIIS
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEE
DTTIAATRTQGVDMKDKFRVNFGFATVAALVTIAFLFIFGKPVTTPKMVSHSFNLIKIIP
CCEEEEECCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
YMFVLIAALAGKNVFAVLMGGIILSGAIGIGYGDFTILGFAKEVYGGFTGMFDIFSLSLL
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
TGGLANMVSKGGGLDWLLYKINKMIKGKKSAELGISSLVALTDAATANNTVSIIVCGELS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHH
KEISKEYKIDPRKTASLLSTFSMAMQGIIPYGAQLLIAGSFTKGSVSPVQIVPYMWYPFI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHH
LAIFGIIFILTPFGNGYLKKHPWDFENSRPAVKGSFENSKPVEKA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]