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The map label for this gene is appB [H]

Identifier: 148379334

GI number: 148379334

Start: 1482050

End: 1483006

Strand: Direct

Name: appB [H]

Synonym: CBO1356

Alternate gene names: 148379334

Gene position: 1482050-1483006 (Clockwise)

Preceding gene: 148379333

Following gene: 148379335

Centisome position: 38.13

GC content: 31.97

Gene sequence:

>957_bases
ATGACCTCAAGATATATACTAAAGAGATTAGGACAGGGTATATTGATTATATTTTTTTTAAGCATAATATCCTTTCTTAT
CATAAATGCTGCGCCGGGAGATCCGGCGGTAGCTATTTATGGTGGGAAAGCTGATAGATTAACCAACTCAGAAAGAGCTA
GAATAGTAAAAAATTATGGGTTAGATAGGCCGGTAATGGATAGATACATAAAATGGTCTGGTCAAATGATAAAAGGAGAC
ATGGGAATATCCTACATAGAAGGAAGATCAGTATCAGAAATATTGAAGGAAAAGATTCCAAATACTATGGTGCTTTTTAT
AAATTCTATACTTTTGATAAGTTTAATTTCAATGATTTTGGGATTAAAAGCCGGATTTAATGAAGGCTCTATATGGGATA
AACTTCTTAGTACTTTAAGTATAGTATTTTATTCTATACCACCTTTTTGGTTAGCTCTCATTTTTATATTAGTATTTTCT
GTTTATACAGGTTGGTTGCCTTCCTCAGGAAATATGAGCATAGGTGGAGAAGGAAGTGTTTTAGATGTACTAAAGCATGC
CATACTACCTATAGCAGTTATAGTAATTACTCATATAGGTGCTTATTCAAGATTTATACAAGAAAAAGTAAAGGAAGAGG
ATAAAAGCTATTATGCTATGGTAGCTAGAGCAAATGGAGTATTAGAAAAGAAAATAAGAAAAGGAATAACTAAAAATGCA
GTAGTTCCTTTTATAAATTATTTAGGCATAACTATTCCTACTTTTTTTAGTGGTTCTGTAATGATAGAAACGGTGTTTTC
CTGGCCGGGCCTTGGAATGCTTACAGTTAAAGCTGCTAACTCAAGAGATTATCCATTGCTTATGGGAGCTATATTTATAA
CTGGAGTTTTAGTTGTTTTATGTATGATAGTTACAGATATAATAGAAATAATAGTTAATCCACATCTTAGAAAGTAG

Upstream 100 bases:

>100_bases
CTATATATGCTGTAAACAAAAAGGTTACAGGAATAAAGGAAAAGATATTAGGACACCATGGAGCTGGATTCATATGGAAT
GCGGAGGAGTGGAAGGTTCA

Downstream 100 bases:

>100_bases
GTGCAAACATGAATAAAAAATCTTATAAAATATGGATTATAGTTTTTTCAATAATAATATTAAGTTCTGTTTTTGCACCC
ATAATAACTAAGTTTAATCC

Product: peptide/opine/nickel uptake ABC transporter, PepT family, permease protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMDRYIKWSGQMIKGD
MGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFS
VYTGWLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHIGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK

Sequences:

>Translated_318_residues
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMDRYIKWSGQMIKGD
MGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFS
VYTGWLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHIGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK
>Mature_317_residues
TSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYGLDRPVMDRYIKWSGQMIKGDM
GISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMILGLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSV
YTGWLPSSGNMSIGGEGSVLDVLKHAILPIAVIVITHIGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNAV
VPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVLCMIVTDIIEIIVNPHLRK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=337, Percent_Identity=26.4094955489614, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787053, Length=316, Percent_Identity=30.0632911392405, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789888, Length=316, Percent_Identity=29.1139240506329, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788504, Length=356, Percent_Identity=30.0561797752809, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787497, Length=317, Percent_Identity=28.0757097791798, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787761, Length=310, Percent_Identity=30.3225806451613, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787550, Length=270, Percent_Identity=27.7777777777778, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35203; Mature: 35071

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYG
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCHHHHHHHHHCC
LDRPVMDRYIKWSGQMIKGDMGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMIL
CCCHHHHHHHHHCCCEEECCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSVYTGWLPSSGNMSIGGEGSV
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LDVLKHAILPIAVIVITHIGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCH
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
CMIVTDIIEIIVNPHLRK
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSRYILKRLGQGILIIFFLSIISFLIINAAPGDPAVAIYGGKADRLTNSERARIVKNYG
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCHHHHHHHHHCC
LDRPVMDRYIKWSGQMIKGDMGISYIEGRSVSEILKEKIPNTMVLFINSILLISLISMIL
CCCHHHHHHHHHCCCEEECCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GLKAGFNEGSIWDKLLSTLSIVFYSIPPFWLALIFILVFSVYTGWLPSSGNMSIGGEGSV
HHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
LDVLKHAILPIAVIVITHIGAYSRFIQEKVKEEDKSYYAMVARANGVLEKKIRKGITKNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCH
VVPFINYLGITIPTFFSGSVMIETVFSWPGLGMLTVKAANSRDYPLLMGAIFITGVLVVL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
CMIVTDIIEIIVNPHLRK
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]