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The map label for this gene is glnL [H]

Identifier: 148378614

GI number: 148378614

Start: 711798

End: 712610

Strand: Reverse

Name: glnL [H]

Synonym: CBO0613

Alternate gene names: 148378614

Gene position: 712610-711798 (Counterclockwise)

Preceding gene: 148378615

Following gene: 148378613

Centisome position: 18.33

GC content: 25.22

Gene sequence:

>813_bases
TTGAGAGTATTAATAATAGATGATGACATAAATGTTCATAAGATTCTTAATAAAATAATAAATCATGAATGCCTGGGGGA
AGTAATAAAGAATACTCCCCTAAATGGGGAAGATGGAGGATTTTTAATAGAGAAAGAAAAACCAGATATTGTTATTGTAG
ATCTTTTAATGCCTGGAAAGGATGGTTTAACTTTAGTTAGAGAATATAAATCTAAATACCCTCATATTCAATATATAATG
TTATCCCAAGTATCCTCTAAAGATATGATAGCTAAAGCCTATGAAAATGGAATTGAGTTTTATATAAGTAAACCTATAAA
TGCAGTTGAGGTTAAATCTATTTTAAAAAAAGTGATTAATAGTGGTATTATGAAAAAAAAATTAGGTAATATAGACTTTA
TATTAAAAAAGGAAGATGAGGATAACTATACATCTAAGATTAAAAGTATAATGAATAAATTAGGCATATTAGGCGAATCA
GGATGCGAAGATATAATAAAAAGTATAGAATTTATAATTGAAAATAATTCTAAAGATACCTATACTCTTAAAGAATTATT
TAAAGAAATATCCGATAAACCCAAATCAATAGAACAAAGAATAAGAAGAACAGCCTTTGTAGCTTTAAATAATATTGCTA
ATTTAGGACTAGAAGATTATATGAATGACATCTTTGTAGAGTTTTCAAATTCTATTTTTAGTTTTGAAGAAGTAAAAAAG
GAAATGGATTATATAAGGGGAAAAAGCTCTGAAAAAGGATCTGTTAATGTTAAAAAATTTTTACAAGGAATAACTTATTG
CATTAATAAATAA

Upstream 100 bases:

>100_bases
AAACCAAGCTAAATGGAATTGTGAAACTAACATCTATAAAAGGAAAAGGTACTAAATTTATGATTTTTATACCCAAAGAT
GTTCTAGAGGGGGAAAGAAT

Downstream 100 bases:

>100_bases
GGTGTATTTGAAATTGTTATAATTATAACAAAATACTTTTTGAAACTTTCCGAAACTTTTTGAAAGCTAGGCTATATAGT
CTAAATAATCAATTAATTCT

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRVLIIDDDINVHKILNKIINHECLGEVIKNTPLNGEDGGFLIEKEKPDIVIVDLLMPGKDGLTLVREYKSKYPHIQYIM
LSQVSSKDMIAKAYENGIEFYISKPINAVEVKSILKKVINSGIMKKKLGNIDFILKKEDEDNYTSKIKSIMNKLGILGES
GCEDIIKSIEFIIENNSKDTYTLKELFKEISDKPKSIEQRIRRTAFVALNNIANLGLEDYMNDIFVEFSNSIFSFEEVKK
EMDYIRGKSSEKGSVNVKKFLQGITYCINK

Sequences:

>Translated_270_residues
MRVLIIDDDINVHKILNKIINHECLGEVIKNTPLNGEDGGFLIEKEKPDIVIVDLLMPGKDGLTLVREYKSKYPHIQYIM
LSQVSSKDMIAKAYENGIEFYISKPINAVEVKSILKKVINSGIMKKKLGNIDFILKKEDEDNYTSKIKSIMNKLGILGES
GCEDIIKSIEFIIENNSKDTYTLKELFKEISDKPKSIEQRIRRTAFVALNNIANLGLEDYMNDIFVEFSNSIFSFEEVKK
EMDYIRGKSSEKGSVNVKKFLQGITYCINK
>Mature_270_residues
MRVLIIDDDINVHKILNKIINHECLGEVIKNTPLNGEDGGFLIEKEKPDIVIVDLLMPGKDGLTLVREYKSKYPHIQYIM
LSQVSSKDMIAKAYENGIEFYISKPINAVEVKSILKKVINSGIMKKKLGNIDFILKKEDEDNYTSKIKSIMNKLGILGES
GCEDIIKSIEFIIENNSKDTYTLKELFKEISDKPKSIEQRIRRTAFVALNNIANLGLEDYMNDIFVEFSNSIFSFEEVKK
EMDYIRGKSSEKGSVNVKKFLQGITYCINK

Specific function: Member of the two-component regulatory system glnL/glnK that positively regulates the expression of the glsA-glnT operon in response to glutamine. GlnL binds the promoter region of glsA- glnT in vitro [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=216, Percent_Identity=24.0740740740741, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR013972 [H]

Pfam domain/function: PF00072 Response_reg; PF08664 YcbB [H]

EC number: NA

Molecular weight: Translated: 30847; Mature: 30847

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLIIDDDINVHKILNKIINHECLGEVIKNTPLNGEDGGFLIEKEKPDIVIVDLLMPGK
CEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEEECCC
DGLTLVREYKSKYPHIQYIMLSQVSSKDMIAKAYENGIEFYISKPINAVEVKSILKKVIN
CCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
SGIMKKKLGNIDFILKKEDEDNYTSKIKSIMNKLGILGESGCEDIIKSIEFIIENNSKDT
CCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
YTLKELFKEISDKPKSIEQRIRRTAFVALNNIANLGLEDYMNDIFVEFSNSIFSFEEVKK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
EMDYIRGKSSEKGSVNVKKFLQGITYCINK
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLIIDDDINVHKILNKIINHECLGEVIKNTPLNGEDGGFLIEKEKPDIVIVDLLMPGK
CEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEEEECCC
DGLTLVREYKSKYPHIQYIMLSQVSSKDMIAKAYENGIEFYISKPINAVEVKSILKKVIN
CCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
SGIMKKKLGNIDFILKKEDEDNYTSKIKSIMNKLGILGESGCEDIIKSIEFIIENNSKDT
CCHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
YTLKELFKEISDKPKSIEQRIRRTAFVALNNIANLGLEDYMNDIFVEFSNSIFSFEEVKK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHH
EMDYIRGKSSEKGSVNVKKFLQGITYCINK
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8011666; 7704254; 9384377 [H]