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The map label for this gene is thiK

Identifier: 148378453

GI number: 148378453

Start: 538012

End: 538803

Strand: Reverse

Name: thiK

Synonym: CBO0450

Alternate gene names: 148378453

Gene position: 538803-538012 (Counterclockwise)

Preceding gene: 148378454

Following gene: 148378452

Centisome position: 13.86

GC content: 33.71

Gene sequence:

>792_bases
ATGAAAAATAAAAATGTTATACAAAAAATGAGAGAAAAAACTCCACTTATTCACTGCATAACTAACTATGTTACTATAAA
CGATTGTGCTAATATACTTCTTTCCTTTGGTGCTTCTCCTGCTATGTGTGAAGCCTATGACGAAGTTTATGATTTTGTAT
CTATATCTTCTGCTTTATATATAAATTTAGGAACTTTAACTAAAGAGCAGGAGACTGCAGCCGTACTTGCTTCCATCTCT
GCCAAGAATCATAATGTGCCTGTAGTTATAGACCCTGTAGGATGCCCTGCTATAAAAAGAAAAGTTGAAGTTATAAATAG
AATCGCTGAAGTTGGAAGAATAGATATTATCAAAGGAAACATAGGAGAAATTAAATTTTTAGCAGGAATGGATTCTGAAA
CTAGAGGGGTAGATTCTCTAGATAATGGTGAAAATGCTTTAGATGCTTGCACACAATTAGCAAAAAAATATAATTGCATT
GTAGCCGCTACTGGTGAAAAAGATTTTGTATCCGATGGGAAAAGAGGTTCTGTAATAAAAAATGGTACAGAAATGCTAAC
TAAAGTAACTGGAGCTGGTTGTATGCTAGGTGCCCTATGTGCCGCTACCTGCGCAAATTTTGAAGATAAATTAGTATCTA
CTACAGCTGCCATATTATCTATGAACATTGCTGGAGAAAAAGCATACGAAGAAGCACAACTACCAGGTAGTTTTAGAATT
GCTTTAATAGATAATATATATATGATATCTGATGAAGAAATTTGGGAAAGAGGTAATGTAGAATGGAAATAA

Upstream 100 bases:

>100_bases
GAAAATAAAAAGTTTTATAAAATTTATTAATTAAGCAAAAAAACATAAACAATCTTTTTTACTAAAATTATATATAATTT
AAATAAAAGGAGCGATTTAT

Downstream 100 bases:

>100_bases
ATTATGAACTTTATTTAATTACAGATAGAAGATTTTTAAAAGGGCGTCAATTAAAAAAAGTAGTGGAAGACGCTATCCTA
GGAGGAGTAACTATAGTACA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 1; TH kinase 1; Thz kinase 1

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKNKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGEKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEEAQLPGSFRI
ALIDNIYMISDEEIWERGNVEWK

Sequences:

>Translated_263_residues
MKNKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGEKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEEAQLPGSFRI
ALIDNIYMISDEEIWERGNVEWK
>Mature_263_residues
MKNKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALYINLGTLTKEQETAAVLASIS
AKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGNIGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCI
VAATGEKDFVSDGKRGSVIKNGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEEAQLPGSFRI
ALIDNIYMISDEEIWERGNVEWK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=251, Percent_Identity=37.4501992031873, Blast_Score=159, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=294, Percent_Identity=31.9727891156463, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM1_CLOB1 (A7FPT1)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382842.1
- ProteinModelPortal:   A7FPT1
- SMR:   A7FPT1
- STRING:   A7FPT1
- GeneID:   5395930
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0491
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   CLSK950618
- BioCyc:   CBOT441770:CLB_0491-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28455; Mature: 28455

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 118-118 BINDING 164-164 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALY
CCCHHHHHHHHHCCCEEEHHHHCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
INLGTLTKEQETAAVLASISAKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGN
EEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECC
IGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCIVAATGEKDFVSDGKRGSVIK
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCCCCCCEEC
NGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEEAQLPGSFRI
CHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
ALIDNIYMISDEEIWERGNVEWK
EEEEEEEEECCHHHHHCCCCCCC
>Mature Secondary Structure
MKNKNVIQKMREKTPLIHCITNYVTINDCANILLSFGASPAMCEAYDEVYDFVSISSALY
CCCHHHHHHHHHCCCEEEHHHHCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEE
INLGTLTKEQETAAVLASISAKNHNVPVVIDPVGCPAIKRKVEVINRIAEVGRIDIIKGN
EEECCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECC
IGEIKFLAGMDSETRGVDSLDNGENALDACTQLAKKYNCIVAATGEKDFVSDGKRGSVIK
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCHHHCCCCCCCEEC
NGTEMLTKVTGAGCMLGALCAATCANFEDKLVSTTAAILSMNIAGEKAYEEAQLPGSFRI
CHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEE
ALIDNIYMISDEEIWERGNVEWK
EEEEEEEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA