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The map label for this gene is thiE

Identifier: 148378452

GI number: 148378452

Start: 537404

End: 538021

Strand: Reverse

Name: thiE

Synonym: CBO0449

Alternate gene names: 148378452

Gene position: 538021-537404 (Counterclockwise)

Preceding gene: 148378453

Following gene: 148378451

Centisome position: 13.84

GC content: 30.42

Gene sequence:

>618_bases
ATGGAAATAAATTATGAACTTTATTTAATTACAGATAGAAGATTTTTAAAAGGGCGTCAATTAAAAAAAGTAGTGGAAGA
CGCTATCCTAGGAGGAGTAACTATAGTACAAGTTAGAGAAAAAGATGTATCTACTAGAGAATTTTATAATGTAGCTAAAG
AAGTAAAGGAAGTAACAGACTATTACAAGGTGCCTATAATTATAAATGATAGATTAGATATTGCTCAAGCTATAGATGCT
AGCGGCGTTCATCTGGGTCAAAAGGATATGCATCTTAATATAGCTAGAGAAATTTTAGGCAAGGATAAAATAATAGGCAT
ATCTGTAGGAAATGTTAAAGAGGCCTTGCAAGCACAAAATAATGGAGCAGATTATCTAGGAATAGGAACTATATTTCCTA
CAGGTAGCAAGAAAGATGTAGATGCAATCATTGGTATTGATGGACTATCAAAAATAAAAGATAGCATATCTATCCCTTCT
GTAGCCATAGGTGGCATAAATAAAACTAACTTTAAAGATGTTTTAAAAACTGGTATAGAAGGAATTTCTGTAATTTCAGC
TATATTAGATGAAGATGATATAAAACTTGCTGCTAACAATCTTTTAATAAATAAATAA

Upstream 100 bases:

>100_bases
CATACGAAGAAGCACAACTACCAGGTAGTTTTAGAATTGCTTTAATAGATAATATATATATGATATCTGATGAAGAAATT
TGGGAAAGAGGTAATGTAGA

Downstream 100 bases:

>100_bases
TTATTTTTATTTGTTATTTTAACTGAAAATAGATTTAATTTTAATATAACAAAGATAAAAAATATCAGTGTATTTTTTTA
TTCTGGTAATGTTAAAATTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MEINYELYLITDRRFLKGRQLKKVVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALQAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK

Sequences:

>Translated_205_residues
MEINYELYLITDRRFLKGRQLKKVVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALQAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK
>Mature_205_residues
MEINYELYLITDRRFLKGRQLKKVVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTDYYKVPIIINDRLDIAQAIDA
SGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALQAQNNGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPS
VAIGGINKTNFKDVLKTGIEGISVISAILDEDDIKLAANNLLINK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=181, Percent_Identity=30.3867403314917, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=220, Percent_Identity=40.4545454545455, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CLOB1 (A7FPT0)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382841.1
- ProteinModelPortal:   A7FPT0
- STRING:   A7FPT0
- GeneID:   5394837
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0490
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   PRK00043
- BioCyc:   CBOT441770:CLB_0490-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22406; Mature: 22406

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINYELYLITDRRFLKGRQLKKVVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTD
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YYKVPIIINDRLDIAQAIDASGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALQAQN
HEECCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCC
NGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPSVAIGGINKTNFKDVLKTGIE
CCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCH
GISVISAILDEDDIKLAANNLLINK
HHHHHHHHHCCCCCEEEECEEEECC
>Mature Secondary Structure
MEINYELYLITDRRFLKGRQLKKVVEDAILGGVTIVQVREKDVSTREFYNVAKEVKEVTD
CCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YYKVPIIINDRLDIAQAIDASGVHLGQKDMHLNIAREILGKDKIIGISVGNVKEALQAQN
HEECCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCC
NGADYLGIGTIFPTGSKKDVDAIIGIDGLSKIKDSISIPSVAIGGINKTNFKDVLKTGIE
CCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCH
GISVISAILDEDDIKLAANNLLINK
HHHHHHHHHCCCCCEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA