Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 148360988

GI number: 148360988

Start: 490079

End: 490546

Strand: Reverse

Name: ogt [H]

Synonym: LPC_2948

Alternate gene names: 148360988

Gene position: 490546-490079 (Counterclockwise)

Preceding gene: 148360987

Following gene: 148360990

Centisome position: 13.72

GC content: 43.38

Gene sequence:

>468_bases
TTGAATGGTAAAATGCTCCTCGTGTCTATTTTTAATACTCCTGTGGGTAACCTGGAAGTAACCCATGATGAGCATTTTAT
TTTCAATGCGTCCTTCACAGACAAATCGGGCACTCCAGCCCAAAACCAATTGTCCCATTTAATTGCCAGTGAATTGAAGG
CTTACTTCGACGATCCCCATCACCGTTTTCAGCTCCCTCTGAAACCTCAGGGAAGCCTCTTCCAGCAAAAGGTTTGGAAT
ACGTTGTTAGTGATTCCCGTTGGCAGAACAGTTACCTATGGAGAACTTGCTACAAAACTGCAAAGTAGTCCAAGAGCAGT
TGGACAAGCCTGTAAACGCAACCCACTGGCTTTATTTGTTCCCTGTCATAGAGTAGTAGGGAAGCATGATCTGGGAGGAT
ATATGGGAAATCCAAACGCCATTTCTTATAAAAAAAATCTGCTCGCGCATGAAGCAAAAATCATCTGA

Upstream 100 bases:

>100_bases
TGGTGACGTTCGTCGTGCCAAATTATATTACTTAAGAAATCTTGCTGGCCGCGCAGCTCGTATTAAAGAAAAATTATCTG
GGAAGAAAGGCGATTAATTC

Downstream 100 bases:

>100_bases
TGGATTAAGTTTATAATAATCAATATTACATTGATCAGAACTTACGAAAACTCCAAGGCAAAAAATATTTTTAACGAAGA
TACTAGCAAGTCGTGTGAGA

Product: methylated DNA protein cysteine S- methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNGKMLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLIASELKAYFDDPHHRFQLPLKPQGSLFQQKVWN
TLLVIPVGRTVTYGELATKLQSSPRAVGQACKRNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII

Sequences:

>Translated_155_residues
MNGKMLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLIASELKAYFDDPHHRFQLPLKPQGSLFQQKVWN
TLLVIPVGRTVTYGELATKLQSSPRAVGQACKRNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII
>Mature_155_residues
MNGKMLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLIASELKAYFDDPHHRFQLPLKPQGSLFQQKVWN
TLLVIPVGRTVTYGELATKLQSSPRAVGQACKRNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=92, Percent_Identity=45.6521739130435, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1787596, Length=169, Percent_Identity=32.5443786982249, Blast_Score=76, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=67, Percent_Identity=50.7462686567164, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17202; Mature: 17202

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKMLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLIASELKAYFDDPH
CCCCEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRAVGQACKRNPLALFV
CEEECCCCCCCHHHHHHHCCEEEEEECCCEEEHHHHHHHHCCCCHHHHHHHCCCCCEEEE
PCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGKMLLVSIFNTPVGNLEVTHDEHFIFNASFTDKSGTPAQNQLSHLIASELKAYFDDPH
CCCCEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
HRFQLPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRAVGQACKRNPLALFV
CEEECCCCCCCHHHHHHHCCEEEEEECCCEEEHHHHHHHHCCCCHHHHHHHCCCCCEEEE
PCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII
EHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]