Click here to switch to the map view.

The map label for this gene is tphA [H]

Identifier: 148360926

GI number: 148360926

Start: 548469

End: 549725

Strand: Reverse

Name: tphA [H]

Synonym: LPC_2886

Alternate gene names: 148360926

Gene position: 549725-548469 (Counterclockwise)

Preceding gene: 148360925

Following gene: 148360944

Centisome position: 15.37

GC content: 39.14

Gene sequence:

>1257_bases
ATGAATCAAAACACCAAACACAGCATTATTGCTGGATTATACGGAAATGCTTTGGAGTGGTATGATTTCTTGCTCTATGC
CAGTTTTGCTCCTGTCTTTGCTGAAATTTTTTTTCCTTCAAAAATCCATTTTGTTTCTCTTATAGCAACATTTAGCTTAT
TTGCTATAGGATTCATTATGCGCCCAATTGGTGGGCTTTTGCTTGGGCACTATGCTGATCATGTAGGACGGCGGAAAGCA
TTAATACTATCTATGTCAGTAATGACCATTTCTACCGCATTAATTGCGGTATTACCTGATTTTAATTCGTGGGGAATTAT
CGCCCCACTTTTATTTTCTATTTTACGGCTCATTCAGGGTCTTGCAGTGGGAGGCGAACTGCCTGGCTCGACCACCTATT
TGATTGAGCATATGTTTCAACAAAGCCGGGAGCTCGCTGGAAGCCTCGTGTTAAGTAGCGCTTTTTTAGGTATTTTTATT
GGAGCGCTTACCGCATCAATATTCAGCACATTATTTACAGGTGATGCTCTATTAAATTGGGGGTGGAGGTATGCGTATCT
GTTAGGAGGTATTTTAGGAATTTTGGGCATTTATTTGCGTATTAAAAGCTTTGAATCACCCACATTCCTAAAGGATAAGC
ATACTGCTGAACTTCCTGCAAAAGTCGTATTCACGCGATTTAAACAGCAACTGTTTTTATCTGTGATAATTACTAGCATT
CTTGCCATGTCTAATTATGTACTTATCGCCTATGTCACAACTTTTTTAGTCAAATCAGAGTATTTTCTCTTGAAAGATGC
GTTATTAATCAACTTAATTGCCCTTGCTCTTTTGACAGTTTTAATACCGCTGATGGGATTTCTTTCTGATGTTGTGGGAC
GCAAGCCTATTTTCTTAATCGGTTTATTTTCTCTTTGCATATTAATCTTCCCATTTTTTTGGCTCTTATTAAGTGGTAGT
TGGTGGAAAGCATTATGCAGCGAATTATTACTAGCGATAGCTTTAGCCCCAATCAATGCGACAGTACCCACTATTATTGC
AGAAATGTTTCCCACCAGTGTACGGGCGAGTGGGATTTCCATATCCTATAACATTGGCCAGGCTTTGTTTGGCGGTACCA
TGCCTTTGGTTGCGTTTACCCTGATTGAATTAAGCAGCAATAAAATGGCTCCGGCTTGGTACCTGTTCCTATGGATCCTT
ATCGTGATACCAATAACTATTTCCATTCGTGAAAGCTATCAAAAAGAACTTACTTAA

Upstream 100 bases:

>100_bases
GCAACTCCGGCCGCTATTTATTAAAAACAAACAACCAAATTAATCCTTTTACTCCAGGAATTCTGAATGGGTTTACTTTA
AACGACCATATTGCATAATT

Downstream 100 bases:

>100_bases
AACATTAGATTGAGATAAATGGACATAATTTCTATCGATTGAATAAGCAACAATAGATTTAAATCAGTTTTTTAGCAGAA
AAAATCCAGCATTAATAGCC

Product: proline/betaine transport protein like protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRELAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFSLCILIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPAWYLFLWIL
IVIPITISIRESYQKELT

Sequences:

>Translated_418_residues
MNQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRELAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFSLCILIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPAWYLFLWIL
IVIPITISIRESYQKELT
>Mature_418_residues
MNQNTKHSIIAGLYGNALEWYDFLLYASFAPVFAEIFFPSKIHFVSLIATFSLFAIGFIMRPIGGLLLGHYADHVGRRKA
LILSMSVMTISTALIAVLPDFNSWGIIAPLLFSILRLIQGLAVGGELPGSTTYLIEHMFQQSRELAGSLVLSSAFLGIFI
GALTASIFSTLFTGDALLNWGWRYAYLLGGILGILGIYLRIKSFESPTFLKDKHTAELPAKVVFTRFKQQLFLSVIITSI
LAMSNYVLIAYVTTFLVKSEYFLLKDALLINLIALALLTVLIPLMGFLSDVVGRKPIFLIGLFSLCILIFPFFWLLLSGS
WWKALCSELLLAIALAPINATVPTIIAEMFPTSVRASGISISYNIGQALFGGTMPLVAFTLIELSSNKMAPAWYLFLWIL
IVIPITISIRESYQKELT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=29.9065420560748, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=29.2857142857143, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=30.272952853598, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=390, Percent_Identity=32.8205128205128, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082404, Length=326, Percent_Identity=26.0736196319018, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=26.9058295964126, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46184; Mature: 46184

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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EEEEHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]