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The map label for this gene is ytbE [H]

Identifier: 148360867

GI number: 148360867

Start: 608579

End: 609409

Strand: Reverse

Name: ytbE [H]

Synonym: LPC_2827

Alternate gene names: 148360867

Gene position: 609409-608579 (Counterclockwise)

Preceding gene: 148360866

Following gene: 148360883

Centisome position: 17.04

GC content: 37.55

Gene sequence:

>831_bases
ATGGACCTTGGCTTTCCAACTTTTATATATGGAACCGCCTGGAAAGAAAAGCGTACAAGCGAACTGGTCGAGCTTGCATT
AACTACCGGATTTCGGGGTATAGATACTGCAAACCAAAGAAAACACTATTATGAAGAAGGCGTTGGATATGCCTTGCAAC
AATACTATAAAAAATCAGGTATCTTGCGTGATGATATTTTTTTGCAATCCAAGTTCACCTATGCTCATGGCCAGGATCAT
CGCAAACCTTACGATGATACAGCAAGCCTGACTATTCAAGTCCAGCAATCTTTTGCAAGCAGCCTTGAACATTTAAATAC
TGATTATTTAAATTCCTATATTTTGCATGGCCCTTATTATCAATATCATTTTTCTGATGAAGATAAAGAAGTCTGGAACA
AAATGGAATCCCTGCATAAAGATGGGAAAGTAAAACACCTGGGCATATCCAATGTGAGTTATGAACAGCTAATCGAACTC
ATTTCATTTGCTTCGGTAAAACCCAAGTTTGTTCAAAATAGATGCTTTGCCAGGCTATTATGGGATAAACAAATTCGTAA
CTATTGCCAGGCCCATGGAATTATTTATCAGGGGTTTTCTCTGTTAACGGCCAATCAATATGAGTTAAGGCATCCGAAAC
TTGAGAATCTCAGCAAAAAGTACGAGAAATCGATTGAGCAAATCATTTTTAGATTTTCACAACAGATAGGAATGTTGCCT
TTAACTGGCACGACAAATTTGGATCATATGAAAGAAGATCTTGCCATCCATCAGTTCAGGCTGACTGATGATGAAATCAA
TTTCATTGAAAACATTACCTTTATCCCATAG

Upstream 100 bases:

>100_bases
AAAACCCTATGTAAGACACACAGTCAGCTATGGTTTGCTCTGTTTTTTGAGTCCAAATAGAGTCTGTTTGCAGATAATAA
GTCGAGAAGGAGATTGAACG

Downstream 100 bases:

>100_bases
AATTTTTTTTCGATTTGACTGGGAAATTCCTATTGTAATCTCCTGTAAAAATGGCATTCCCTAACCAAAAAAAATGAACT
TCATGATGAATGCTTCCTGA

Product: aldo/keto reductase-like diketogulonate reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENITFIP

Sequences:

>Translated_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENITFIP
>Mature_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYAHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENITFIP

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=26.962457337884, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=26.4285714285714, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5174695, Length=305, Percent_Identity=26.2295081967213, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI310109920, Length=299, Percent_Identity=25.4180602006689, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=25.4180602006689, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI45446745, Length=299, Percent_Identity=25.4180602006689, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4503285, Length=299, Percent_Identity=25.4180602006689, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=26.1744966442953, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI310109922, Length=284, Percent_Identity=25.7042253521127, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=24.8322147651007, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=25.6849315068493, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=25.6849315068493, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI300116273, Length=283, Percent_Identity=25.0883392226148, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI93277124, Length=301, Percent_Identity=24.5847176079734, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=24.0418118466899, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI87082198, Length=251, Percent_Identity=27.4900398406374, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=28.3828382838284, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71998625, Length=250, Percent_Identity=29.2, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17562292, Length=261, Percent_Identity=26.4367816091954, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17537079, Length=305, Percent_Identity=24.9180327868852, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17566692, Length=296, Percent_Identity=23.6486486486486, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17561298, Length=257, Percent_Identity=27.6264591439689, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552492, Length=282, Percent_Identity=23.758865248227, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17561300, Length=263, Percent_Identity=24.7148288973384, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538386, Length=267, Percent_Identity=25.0936329588015, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17537077, Length=258, Percent_Identity=25.1937984496124, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537075, Length=258, Percent_Identity=23.6434108527132, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17550248, Length=293, Percent_Identity=21.8430034129693, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320079, Length=254, Percent_Identity=30.7086614173228, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322556, Length=268, Percent_Identity=24.6268656716418, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320576, Length=273, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=26.0135135135135, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=28.0701754385965, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=24.8251748251748, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=27.0903010033445, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=25.3424657534247, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356425, Length=261, Percent_Identity=25.6704980842912, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=28.3216783216783, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=21.8855218855219, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=28.0769230769231, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32408; Mature: 32408

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHEECCCEE
QYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHCCHHHHCHHHEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENITFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHCCCCCC
>Mature Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFRGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYAHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHEECCCEE
QYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLIELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKYEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHCCHHHHCHHHEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENITFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]