Click here to switch to the map view.

The map label for this gene is def2 [H]

Identifier: 148360734

GI number: 148360734

Start: 742497

End: 743099

Strand: Reverse

Name: def2 [H]

Synonym: LPC_2683

Alternate gene names: 148360734

Gene position: 743099-742497 (Counterclockwise)

Preceding gene: 148360731

Following gene: 148360736

Centisome position: 20.78

GC content: 36.48

Gene sequence:

>603_bases
ATGGCTTTAGAAATTATTACAATAGAAAAAAAAGAAAACTACCATATGCTAAAAACTCGGGCTGAAAATATTCAATTTCC
CTTGAATCGAGAAGATAAAGATTTAATAGAAGCGATGAAAAAAAAATTATACGCATTGGGAGGGGTTGGTTTGGCAGCAC
CACAAGTCAATTCCGCCAAACAAATTATCGCAGTTTACATCCCTGAAGAAGCTCAGCTTTTACGTGATAATGCGAAAATC
TTTCCAATGCACATCCTAATCAATCCAAATTACGAGCCCCTGCCCCACACTCGTATTATTAGCGACTTTGAAGGTTGTTA
TTCTGTATCCAGTAAAGCAGGGAAAGTGCCAAGATATGATGAAATTCAGCTAAAGTACTATGATGAAGAAGGGAATGTTC
ACCAACAAATCGAAAATGGCTTTTACGCCCGTGTTTTGCAACATGAAATTGATCATCTGAATGGCGTATTAATCACCGAT
CGTTTAACTCCCGATTGTGTACAAGGGACTATTGAAGAAATGATGACTATTCGCCGTGCTGAATTGCCTGTAGAAAAACG
AAAATTATTTGATCAAATCATGGCAAAAAAACTTAAAAAATAA

Upstream 100 bases:

>100_bases
ATTAGGAAAATCATTGCTACTAACCACATTAACGAGAACCAAAAAGAAATCTCAACAACTTCTTATTTTAATAGGCTTCT
TATTAGGGATGTCACCAGTC

Downstream 100 bases:

>100_bases
GGTCGAGTTCCCAAATTATCTTTTATGAATTGACTTCAATGATAAACTGCCCTGGGTAATTTTTTTGCTTGTTCAACCCA
GGTAACAACTTGAGATAATC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLYALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKI
FPMHILINPNYEPLPHTRIISDFEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
RLTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK

Sequences:

>Translated_200_residues
MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLYALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKI
FPMHILINPNYEPLPHTRIISDFEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITD
RLTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK
>Mature_199_residues
ALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLYALGGVGLAAPQVNSAKQIIAVYIPEEAQLLRDNAKIF
PMHILINPNYEPLPHTRIISDFEGCYSVSSKAGKVPRYDEIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDR
LTPDCVQGTIEEMMTIRRAELPVEKRKLFDQIMAKKLKK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=144, Percent_Identity=31.25, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1789682, Length=137, Percent_Identity=32.8467153284672, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24645726, Length=171, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=31.7073170731707, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 23093; Mature: 22962

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLYALGGVGLAAPQVNSAK
CEEEEEEEECCCCCHHHHEHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QIIAVYIPEEAQLLRDNAKIFPMHILINPNYEPLPHTRIISDFEGCYSVSSKAGKVPRYD
EEEEEEECCHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
EIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRA
CEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
ELPVEKRKLFDQIMAKKLKK
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALEIITIEKKENYHMLKTRAENIQFPLNREDKDLIEAMKKKLYALGGVGLAAPQVNSAK
EEEEEEEECCCCCHHHHEHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
QIIAVYIPEEAQLLRDNAKIFPMHILINPNYEPLPHTRIISDFEGCYSVSSKAGKVPRYD
EEEEEEECCHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
EIQLKYYDEEGNVHQQIENGFYARVLQHEIDHLNGVLITDRLTPDCVQGTIEEMMTIRRA
CEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
ELPVEKRKLFDQIMAKKLKK
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]