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The map label for this gene is pntA [H]

Identifier: 148360476

GI number: 148360476

Start: 1057097

End: 1058230

Strand: Reverse

Name: pntA [H]

Synonym: LPC_2416

Alternate gene names: 148360476

Gene position: 1058230-1057097 (Counterclockwise)

Preceding gene: 148360475

Following gene: 148360477

Centisome position: 29.59

GC content: 41.53

Gene sequence:

>1134_bases
ATGATGATTGCAACCTTAATGGAATCAGCTCAGGAAACGCGAGTAGCTATTACTCCTAACTCGGCCAAACAATACATAAA
ACTAGGCTATGATGTAGGGATTGAAAAAAACGCGGGGCTTAGCTCAGATTTTGCTAACGAAGAATTTGAAAAAGCTGGCG
CAATAATATTCGATAGTAAAAGTACATTACTCAAAAAAACACAAATTCTTTTATGTGTTAACGAACCTGATCCCAAAAAC
CTGAATGGCTTACCTCCAGATTCTCTAATAATTGGACATATAGACAATAACCCACTTAGCCCGCTTGTTAAGTGGTGTCT
GGATCAAAAAATGACATTATTTTCAATGAATCTCATCCCCAGGATAAGTCGTGCTCAAAGCATGGACAGCCTATCGTCTC
AGGCTAACCTGGCTGGTTACAGGGCAGTTTTGGAAGCTGCGGCAACATTTCATAGAGCTATACCTATGATGATGACAGCA
GCAGGCATGATACAACCGGCCAAAGCACTTATTCTTGGTGCCGGAGTTGCAGGCTTGCAAGCCATAGCTACTGCGAAACG
TTTAGGTGCAGTGGTTTATGCCTTTGACGTAAGAAAGGCAGCCAAAGAACAGGTTGAAAGTTTAGGCGCTGAATTCATAG
AGGTAATGCAGGAGCAAGATAGTGAAACAGCTGGGGGATATGCCACAGAAACAAGCGAAGAATATAAAAAACTTCAGGCT
GAACTCATTGATCAATATGCAAAAATTGCAGACATTGTAATTTCTACTGCCTTGATACCAGGGCGTCAAGCTCCGGTTTT
ACTTTATAAAAAAACTGTCGAGCAAATGAAACCAGGTTCTGTCATTGTTGATCTCGCAACGTCTCGAGGTGGAAATTGTG
AACTCAGTGTTGCCGATAAAACCATCAAGCATGGTGAGATTACTATAGTGGGATTAAGTAATATGGCAGGTCTCGTCCCT
GCTACGGCAAGTGACCTTTATTCTAATAATCTGGTTCATCTTATAAACCTTTTGGCGAAAAATCCGGCTGAACTTAACTT
AAATCCTGATGATGAGATTATCCAGCAAGCCGTTCTTTGCCATAAAGGAACTTACCTGCCCTTTCAAGGAGTAAAGGAGA
AGCAAAATGCCTGA

Upstream 100 bases:

>100_bases
GCTTGTCCGGATCCAAAGATCCAAAATTGGTATTTTATGCTGTAGAAAATTATTCTAAATTACAATAGGATAGAATAATA
ACGGTAATAAGGATTTTCCC

Downstream 100 bases:

>100_bases
AATTCATACACTTGATAATCCTTATATTACAATATTAACCATTTTCGTACTGGCCTGTTTTGTAGGTTATTATGTGGTTT
GGAAAGTAACACCGGCTTTA

Product: pyridine nucleotide transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDFANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKN
LNGLPPDSLIIGHIDNNPLSPLVKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQEQDSETAGGYATETSEEYKKLQA
ELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQMKPGSVIVDLATSRGGNCELSVADKTIKHGEITIVGLSNMAGLVP
ATASDLYSNNLVHLINLLAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA

Sequences:

>Translated_377_residues
MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDFANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKN
LNGLPPDSLIIGHIDNNPLSPLVKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQEQDSETAGGYATETSEEYKKLQA
ELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQMKPGSVIVDLATSRGGNCELSVADKTIKHGEITIVGLSNMAGLVP
ATASDLYSNNLVHLINLLAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA
>Mature_377_residues
MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDFANEEFEKAGAIIFDSKSTLLKKTQILLCVNEPDPKN
LNGLPPDSLIIGHIDNNPLSPLVKWCLDQKMTLFSMNLIPRISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTA
AGMIQPAKALILGAGVAGLQAIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQEQDSETAGGYATETSEEYKKLQA
ELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQMKPGSVIVDLATSRGGNCELSVADKTIKHGEITIVGLSNMAGLVP
ATASDLYSNNLVHLINLLAKNPAELNLNPDDEIIQQAVLCHKGTYLPFQGVKEKQNA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=352, Percent_Identity=38.6363636363636, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI122939153, Length=352, Percent_Identity=38.6363636363636, Blast_Score=224, Evalue=8e-59,
Organism=Escherichia coli, GI1787887, Length=355, Percent_Identity=41.6901408450704, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17550456, Length=353, Percent_Identity=41.0764872521246, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 40616; Mature: 40616

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDFANEEFEKAGAIIFDSK
CCHHHHHHHHHHCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCC
STLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPLVKWCLDQKMTLFSMNLIP
HHHHEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
RISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTAAGMIQPAKALILGAGVAGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHH
AIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQEQDSETAGGYATETSEEYKKLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQMKPGSVIVDLATSRGGNCELSVADK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCC
TIKHGEITIVGLSNMAGLVPATASDLYSNNLVHLINLLAKNPAELNLNPDDEIIQQAVLC
EECCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
HKGTYLPFQGVKEKQNA
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMIATLMESAQETRVAITPNSAKQYIKLGYDVGIEKNAGLSSDFANEEFEKAGAIIFDSK
CCHHHHHHHHHHCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCC
STLLKKTQILLCVNEPDPKNLNGLPPDSLIIGHIDNNPLSPLVKWCLDQKMTLFSMNLIP
HHHHEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHH
RISRAQSMDSLSSQANLAGYRAVLEAAATFHRAIPMMMTAAGMIQPAKALILGAGVAGLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHH
AIATAKRLGAVVYAFDVRKAAKEQVESLGAEFIEVMQEQDSETAGGYATETSEEYKKLQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ELIDQYAKIADIVISTALIPGRQAPVLLYKKTVEQMKPGSVIVDLATSRGGNCELSVADK
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCC
TIKHGEITIVGLSNMAGLVPATASDLYSNNLVHLINLLAKNPAELNLNPDDEIIQQAVLC
EECCCCEEEEECCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
HKGTYLPFQGVKEKQNA
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]