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The map label for this gene is asnS [H]

Identifier: 148359826

GI number: 148359826

Start: 2700406

End: 2701809

Strand: Direct

Name: asnS [H]

Synonym: LPC_1751

Alternate gene names: 148359826

Gene position: 2700406-2701809 (Clockwise)

Preceding gene: 148359824

Following gene: 148359834

Centisome position: 75.5

GC content: 42.88

Gene sequence:

>1404_bases
ATGACAGAAGTCTTTACTATAAAACAATGTCTCGACGGCGAAATCAGTATTGATGAAACCGTTACGGTAAGAGGATGGGT
GAAAACTCGAAGAGATTCAAAAGCTGGCTTGTCTTTCATCAGCCTTCATGATGGCTCTTGCTTTTCTCCCATACAAATCG
TCGCTACTGATCAGTTATCCAACTACCACAAAGAAGTAACAAAATTAACTGCTGGTTGCTCAATGGTAGCGACTGGAAAA
TTGGTGGCATCGCAAGGAAAAGGGCAATTTTTTGAAATCCAGGCCGAATCCATTGAAGTGGTTGGATGGGTTGAAAACCC
AGATACATATCCTATTCAAGCCAAACGCCATACTCTTGAATTTTTAAGAGAAGTAGCTCATTTGCGCCCCAGAACCAATA
CCATAAGCGCTGTCACCAGAGTGCGTCATTCTTTGGCGCAAGCCATTCACCGTTTTTATCATGAACAAGGTTTTTTTTGG
GTGCATACACCTATCATTACTGCCAGTGACTGCGAGGGGGCAGGAGAAATGTTCAGAGTATCCACTCTGGATTTATTAAA
TATCCCTAAAAATGATAAAGGACAAATCGATTTCAGTAAGGATTTTTTTGGTAGAGAAACGTTCTTAACTGTTTCTGGCC
AACTAAATGTCGAAGCTTATTGTATGGCCATGTCCAAGGTCTACACTTTTGGACCAACATTTCGGGCTGAAAATTCGAAT
ACAAGCCGCCATTTGGCTGAATTCTGGATGATCGAACCTGAAATCGCTTTTGCAAATCTGGAAGACATTTGTAAACTCAG
CCAAAGTATGTTGCGTTATCTCTGCAAAACAGTTCTTGAAGAGCGTGCCGATGATATGGACTTTTTCAATCAATTTGTCG
CGCCAGGATGTATAGAACGTATGGAACATATTGCGGATTCTGAATTTGAAATGATGACCTATACCGATGCGATAAAAGCA
CTTGAGGCGAGTGACCAAAAATTTGAATTTCCAGTTAGTTGGGGATTAGATCTCCAATCAGAGCATGAACGTTACCTGGC
TGAAGTACTTTGCAAAAAGCCTGTCATTGTGACAAATTACCCGCAAGAAATTAAGGGGTTTTACATGCGTCTTAACGACG
ATGGGAAAACGGTAGCCGCCATGGACGTTTTAGCTCCAGGCATCGGAGAGATTATCGGGGGCAGCCAACGCGAAGAGCGA
TTGGAAATACTGGATAGACGCATGGACGAATGCAACCTGAATAAAGAACATTACCAATGGTATAGAGATCTGCGCCGTTA
TGGCACTGTTCCTCATGCCGGGTTTGGCTTGGGCTTTGAACGTCTGATTAGCTATGTTACAGGAGTATCCAATGTGCGAG
ATGTGATACCTTTCCCTCGCACACCAGGTCACGCGGATTATTAA

Upstream 100 bases:

>100_bases
AGGGTTTAGGAAATACTACAAACGGGAGAAAATCAATCCCTCGAAGGATTGGAATATTTCCTAAGCCCTGAATCAATCGT
TTTCGATTTTGGAGTATATA

Downstream 100 bases:

>100_bases
ATCGGTAAAGGTCTAAAAGAGTCATCATCAACTTCAACTGTTTGTTGTTGGGGTTGGTGACTCTCTGGCAGCAATCGCCG
TCTATTTCTTGGCGAAAATA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHDGSCFSPIQIVATDQLSNYHKEVTKLTAGCSMVATGK
LVASQGKGQFFEIQAESIEVVGWVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVEAYCMAMSKVYTFGPTFRAENSN
TSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCKTVLEERADDMDFFNQFVAPGCIERMEHIADSEFEMMTYTDAIKA
LEASDQKFEFPVSWGLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREER
LEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVSNVRDVIPFPRTPGHADY

Sequences:

>Translated_467_residues
MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHDGSCFSPIQIVATDQLSNYHKEVTKLTAGCSMVATGK
LVASQGKGQFFEIQAESIEVVGWVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFW
VHTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVEAYCMAMSKVYTFGPTFRAENSN
TSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCKTVLEERADDMDFFNQFVAPGCIERMEHIADSEFEMMTYTDAIKA
LEASDQKFEFPVSWGLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREER
LEILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVSNVRDVIPFPRTPGHADY
>Mature_466_residues
TEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHDGSCFSPIQIVATDQLSNYHKEVTKLTAGCSMVATGKL
VASQGKGQFFEIQAESIEVVGWVENPDTYPIQAKRHTLEFLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFWV
HTPIITASDCEGAGEMFRVSTLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVEAYCMAMSKVYTFGPTFRAENSNT
SRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCKTVLEERADDMDFFNQFVAPGCIERMEHIADSEFEMMTYTDAIKAL
EASDQKFEFPVSWGLDLQSEHERYLAEVLCKKPVIVTNYPQEIKGFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREERL
EILDRRMDECNLNKEHYQWYRDLRRYGTVPHAGFGLGFERLISYVTGVSNVRDVIPFPRTPGHADY

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=462, Percent_Identity=42.4242424242424, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI4758762, Length=454, Percent_Identity=27.7533039647577, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI45439306, Length=349, Percent_Identity=28.9398280802292, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1787161, Length=464, Percent_Identity=68.9655172413793, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=470, Percent_Identity=24.2553191489362, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556372, Length=435, Percent_Identity=32.4137931034483, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984122, Length=453, Percent_Identity=29.8013245033113, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17551876, Length=347, Percent_Identity=27.0893371757925, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319872, Length=476, Percent_Identity=34.453781512605, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=464, Percent_Identity=29.7413793103448, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=344, Percent_Identity=27.0348837209302, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922416, Length=457, Percent_Identity=36.980306345733, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19921528, Length=467, Percent_Identity=28.9079229122056, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17136276, Length=344, Percent_Identity=26.1627906976744, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 53213; Mature: 53082

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHDGSCFSPIQIVATDQLS
CCCEEEHHHHCCCCCCCCCEEEEEHHHHHCCCCHHCCEEEEECCCCCCCCEEEEEEHHHH
NYHKEVTKLTAGCSMVATGKLVASQGKGQFFEIQAESIEVVGWVENPDTYPIQAKRHTLE
HHHHHHHHHHHCCCEEECCHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHH
FLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFWVHTPIITASDCEGAGEMFRV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCEEEE
STLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVEAYCMAMSKVYTFGPTFRAENSN
EEEHEEECCCCCCCCEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCC
TSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCKTVLEERADDMDFFNQFVAPGCIER
CHHHHHHHHEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
MEHIADSEFEMMTYTDAIKALEASDQKFEFPVSWGLDLQSEHERYLAEVLCKKPVIVTNY
HHHHCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECC
PQEIKGFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREERLEILDRRMDECNLNKEHYQW
CHHHCEEEEEECCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
YRDLRRYGTVPHAGFGLGFERLISYVTGVSNVRDVIPFPRTPGHADY
HHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TEVFTIKQCLDGEISIDETVTVRGWVKTRRDSKAGLSFISLHDGSCFSPIQIVATDQLS
CCEEEHHHHCCCCCCCCCEEEEEHHHHHCCCCHHCCEEEEECCCCCCCCEEEEEEHHHH
NYHKEVTKLTAGCSMVATGKLVASQGKGQFFEIQAESIEVVGWVENPDTYPIQAKRHTLE
HHHHHHHHHHHCCCEEECCHHHCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHH
FLREVAHLRPRTNTISAVTRVRHSLAQAIHRFYHEQGFFWVHTPIITASDCEGAGEMFRV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCEEEE
STLDLLNIPKNDKGQIDFSKDFFGRETFLTVSGQLNVEAYCMAMSKVYTFGPTFRAENSN
EEEHEEECCCCCCCCEEECHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCC
TSRHLAEFWMIEPEIAFANLEDICKLSQSMLRYLCKTVLEERADDMDFFNQFVAPGCIER
CHHHHHHHHEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHH
MEHIADSEFEMMTYTDAIKALEASDQKFEFPVSWGLDLQSEHERYLAEVLCKKPVIVTNY
HHHHCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECC
PQEIKGFYMRLNDDGKTVAAMDVLAPGIGEIIGGSQREERLEILDRRMDECNLNKEHYQW
CHHHCEEEEEECCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
YRDLRRYGTVPHAGFGLGFERLISYVTGVSNVRDVIPFPRTPGHADY
HHHHHHHCCCCCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA