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The map label for this gene is menE [H]

Identifier: 148359772

GI number: 148359772

Start: 2638082

End: 2639461

Strand: Direct

Name: menE [H]

Synonym: LPC_1697

Alternate gene names: 148359772

Gene position: 2638082-2639461 (Clockwise)

Preceding gene: 148359771

Following gene: 148359773

Centisome position: 73.76

GC content: 39.42

Gene sequence:

>1380_bases
ATGCTGTTTTTGGCATCAGATGTTCCAAACAAAGTAGCAATTACCCAGGACAATCGAGCAATAACTTTTGATGAATTAAC
GCATGAAGTTTTAGCAAATTCAAAGCAACTCCTGAAACTTCCAAAAACCATCATTGTCCTCCATGCAACACCTGAGATTG
GATTTATTATTCAACTATTAGCCTGTTTGGAAACTGGTCGCCCCATTGCCCTTTTCCCCAATTCCATTTCAGAGGAAGAA
AAGCAAAGACGACTGTCTCTTTTGGGTAATGCCGTAATGATAAATGAAGAAGGGGAATTGCAGGAAATATGTGAAAATAA
AACGATCCAGCCCCATCCCCAGACAGCACTTATTCTCTTCACCTCAGGAAGCACAGGATCAGTAAAAGCAGTTCAACTAT
CAAGCATCAACATAATAGCCAACTGCCATGCGGTCATAAAGGCGCTGGAATTTAAAAAAGTTCAAGATCAATTGCTCTTT
TTACCTTTATCCTATTCTTTTGGCCTTCTGGGCCAATTGCTCCCTGGTTTAATGTTTGGCCTTAGCACCCGACTAATCTC
TCATTTTACAGACATTAAAATGCTAATGGAGCAAGGTACCGTTCCTCAAATGTGGAGTGGCGTACCTTCTCACTGGGTAG
CTATCAATGCCATAGCAAAACGATACCCTGATAGCGCAGCCAAAATTAACGCGATTGTTTCTGCTGGCGCTCCTTTATCC
ACTACACTCAGAGCGGATTTGAAACAAATATTTCCTAACGCTATCATCTATAACAATTACGGCTTAACTGAAGCGTCTCC
TAGAGTGCTGACTTATTCCAGTAAGGATCCTTTATTCACAGAAAATTACGCAGGTTATCCTGTTGGCGATTGGAAGGTCA
AACTTTCTGCTGAACATGAATTATTAATTAGCGGCACTCAAATGATGCTGGGTTATCTTGGTGAAAAAGATAGTACTAAA
ATACAAAATGGCTGGTTATCAACAGGAGATATTGCAGAAATATTACCCAATGGGTTAGTTGCAATCAAAGGCCGTCGAGA
CAGTCTTGTTAATATTGGAGGAGAAAAGGTTAACCTCTCTGAGATCGAGCAAACAATTTGCCAAATTGATGTAATAAAAG
AGGCCATTGTTATCCCTCAGGAAGACAAGATATATGGGACACGATTAATTGTCTGTATCGAAAAAAAATCGCTGCCTTCT
TCCATTTCTGAACAAAATTTAATAGAAAAAATACAAACGCATCTACTACCCAGGAAATTGCCCATTCAATTACAGCTCAT
GTCATCCCTACCCAGAAATCAACATGGGAAGCTTGATAGAAAAGCGTTGGTTTTGAATATTGGCGGTTTAGATAATAAGG
AAAAAAATAATGCTAAGTAA

Upstream 100 bases:

>100_bases
AGCGGAAAAATTCGCAGGAATTTTTGCCGAAAACATCTTTTGGATAAAACTCTGCCAATAGTAGCTACCTGGCAACTCAA
TAAAATTGAGGAATAAAATC

Downstream 100 bases:

>100_bases
TCGAATTGAACAAGTACTGGAACGAATTGGCCTTACCAATCTTCCTGAAAACAAACAGGCAAAACTTGAGCAAGGGGGCT
TGGATAGTTTGATGCTGGCC

Product: acyl CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLF
LPLSYSFGLLGQLLPGLMFGLSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQTICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIGGLDNKEKNNAK

Sequences:

>Translated_459_residues
MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLF
LPLSYSFGLLGQLLPGLMFGLSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQTICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIGGLDNKEKNNAK
>Mature_459_residues
MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTIIVLHATPEIGFIIQLLACLETGRPIALFPNSISEEE
KQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILFTSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLF
LPLSYSFGLLGQLLPGLMFGLSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
TTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHELLISGTQMMLGYLGEKDSTK
IQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLSEIEQTICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPS
SISEQNLIEKIQTHLLPRKLPIQLQLMSSLPRNQHGKLDRKALVLNIGGLDNKEKNNAK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=380, Percent_Identity=26.0526315789474, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI187761343, Length=380, Percent_Identity=26.0526315789474, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI156151445, Length=359, Percent_Identity=27.5766016713092, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI145693145, Length=351, Percent_Identity=25.0712250712251, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788107, Length=358, Percent_Identity=26.8156424581006, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1786801, Length=526, Percent_Identity=25.4752851711027, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786810, Length=392, Percent_Identity=21.6836734693878, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788595, Length=356, Percent_Identity=26.4044943820225, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=403, Percent_Identity=25.0620347394541, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=366, Percent_Identity=25.6830601092896, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=346, Percent_Identity=24.2774566473988, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=375, Percent_Identity=25.8666666666667, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=360, Percent_Identity=25.5555555555556, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=365, Percent_Identity=25.7534246575342, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=301, Percent_Identity=24.9169435215947, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=430, Percent_Identity=24.1860465116279, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71985884, Length=371, Percent_Identity=24.7978436657682, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319699, Length=344, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=350, Percent_Identity=27.1428571428571, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=27.7310924369748, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355181, Length=379, Percent_Identity=26.1213720316623, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648676, Length=379, Percent_Identity=22.6912928759894, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50598; Mature: 50598

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTIIVLHATPEIGFIIQLL
CEEEECCCCCCEEEECCCCEEEHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHH
ACLETGRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILF
HHHHCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCEEEEEE
TSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLFLPLSYSFGLLGQLLPGLMFG
ECCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHEECCCCCH
TTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHE
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCE
LLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLS
EEEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCEECHH
EIEQTICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPSSISEQNLIEKIQTHLLPRKL
HHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
PIQLQLMSSLPRNQHGKLDRKALVLNIGGLDNKEKNNAK
CEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLFLASDVPNKVAITQDNRAITFDELTHEVLANSKQLLKLPKTIIVLHATPEIGFIIQLL
CEEEECCCCCCEEEECCCCEEEHHHHHHHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHH
ACLETGRPIALFPNSISEEEKQRRLSLLGNAVMINEEGELQEICENKTIQPHPQTALILF
HHHHCCCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCCCCCEEEEEE
TSGSTGSVKAVQLSSINIIANCHAVIKALEFKKVQDQLLFLPLSYSFGLLGQLLPGLMFG
ECCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHH
LSTRLISHFTDIKMLMEQGTVPQMWSGVPSHWVAINAIAKRYPDSAAKINAIVSAGAPLS
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHEECCCCCH
TTLRADLKQIFPNAIIYNNYGLTEASPRVLTYSSKDPLFTENYAGYPVGDWKVKLSAEHE
HHHHHHHHHHCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCE
LLISGTQMMLGYLGEKDSTKIQNGWLSTGDIAEILPNGLVAIKGRRDSLVNIGGEKVNLS
EEEECHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCEECHH
EIEQTICQIDVIKEAIVIPQEDKIYGTRLIVCIEKKSLPSSISEQNLIEKIQTHLLPRKL
HHHHHHHHHHHHHHHHCCCCCCCEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
PIQLQLMSSLPRNQHGKLDRKALVLNIGGLDNKEKNNAK
CEEEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA