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The map label for this gene is ycjG [C]

Identifier: 148359519

GI number: 148359519

Start: 2293773

End: 2294876

Strand: Reverse

Name: ycjG [C]

Synonym: LPC_1429

Alternate gene names: 148359519

Gene position: 2294876-2293773 (Counterclockwise)

Preceding gene: 148359521

Following gene: 148359518

Centisome position: 64.17

GC content: 40.49

Gene sequence:

>1104_bases
TTGAATATTAAAGAAATCCATATCGCAGAACTAACTATTCCATTAAGCCGTCCGTTTATTACAGCGGTGAGGCGTACAGA
ATGCGTTGATGATGTCGTGATATTAATAAAAACGGATTGTGGAAAGATTGGTTATGGTTCAGCTGCATCAACACCTGCAA
TAACTGGGGATAGCAAGGAATCCATTATTGCTGCTATTAAAAACGCCTTGGGGCCTCCTCTTATCGGACGTGATATTTCT
GCATTCAATTTATTACTTGAAATGAATGACCGGGCTATAGGAGGTAATTCCTCAGCCAAAGCTGCGATTGATATTGCTTT
GCATGATTTATTTGCCCAATATTGTGGCTTGCCTCTTTATAAACAATTGGGAGGAAATACCAATTGCATCAACTCATGTA
TTACGATAAGTGTTAAAGAAGTTGAGGAGATGGTGGGTGATGCGATTGATTTAATTCAGCGAGGGCACAAAACCCTGAAA
ATCAAATTGGGATTAAATCCAGTGGACGACATTAATCGAATTCGAGCTATTCGTCAGGCAGTAGGCAATGACATTACTCT
GCTAGCAGATGCAAATCAGGGATGGTCTTATGAAGAGGCATTAATCGCCATTACTGCGTTCAAAAAAGAGAATTTGAATG
TTCCTCTGGTGGAGCAACCGGTTAGTGCTCGAAATCTGCCTGATTTAAAGACGATTAGTGATCAGGTTGATTGTGCAATT
ATCGCTGACGAAGCGTGTTTTTCACCAGAAGATGCATTGAATATCGCAAAGATTAATGCTTGTGATGGGGTCAATATAAA
GTTGATGAAATCAGGAGGCATTGAGAAAGCTCAATCTATCTACCATATTGCTCAAGCGGCCAAAATGCAAATAATGGTTG
GATGTATGCTGGAGTCGCCGATTGGAGTTGCTGCAATAGCCAGTTTTGCATTAAGTAAGCCCGATATTTCTTATGCAGAT
CTCGATCCTATTTTTTTGATCCGCGAGAATTATATTCTTGGTGGGGCACAAAGTCTCAGCAATAAAATTATCCTGGCAGA
GAAACCTGGATTAGGGATTGAAGGCATCACACAAGGACTAAATCTAATTGGGGTGATCCGCTAA

Upstream 100 bases:

>100_bases
ATTATATTGGTTTCTAAAAGCAGAACAATGCATAATAAAATTGGGTAAAAAATTGAGATTGTTATGATCCCCTGCAACCT
ATAAATGAACAGGTGCTTGT

Downstream 100 bases:

>100_bases
TGTCTTTCTACATTCAGCTAGCCAGTTTATCTATTATTATTCTTTATGTTTTACTGTTATTTCGTAACGTTGATCCATTA
GTAGTGACAGCAGTTTGCGT

Product: chloromuconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIGYGSAASTPAITGDSKESIIAAIKNALGPPLIGRDIS
AFNLLLEMNDRAIGGNSSAKAAIDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLK
IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVEQPVSARNLPDLKTISDQVDCAI
IADEACFSPEDALNIAKINACDGVNIKLMKSGGIEKAQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYAD
LDPIFLIRENYILGGAQSLSNKIILAEKPGLGIEGITQGLNLIGVIR

Sequences:

>Translated_367_residues
MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIGYGSAASTPAITGDSKESIIAAIKNALGPPLIGRDIS
AFNLLLEMNDRAIGGNSSAKAAIDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLK
IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVEQPVSARNLPDLKTISDQVDCAI
IADEACFSPEDALNIAKINACDGVNIKLMKSGGIEKAQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYAD
LDPIFLIRENYILGGAQSLSNKIILAEKPGLGIEGITQGLNLIGVIR
>Mature_367_residues
MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIGYGSAASTPAITGDSKESIIAAIKNALGPPLIGRDIS
AFNLLLEMNDRAIGGNSSAKAAIDIALHDLFAQYCGLPLYKQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLK
IKLGLNPVDDINRIRAIRQAVGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVEQPVSARNLPDLKTISDQVDCAI
IADEACFSPEDALNIAKINACDGVNIKLMKSGGIEKAQSIYHIAQAAKMQIMVGCMLESPIGVAAIASFALSKPDISYAD
LDPIFLIRENYILGGAQSLSNKIILAEKPGLGIEGITQGLNLIGVIR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=330, Percent_Identity=29.0909090909091, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39229; Mature: 39229

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIGYGSAASTPAITGDSKE
CCCCEEEEEEEEEECCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
SIIAAIKNALGPPLIGRDISAFNLLLEMNDRAIGGNSSAKAAIDIALHDLFAQYCGLPLY
HHHHHHHHHCCCCCCCCCHHHEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCHHH
KQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLKIKLGLNPVDDINRIRAIRQA
HHHCCCCHHCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHH
VGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVEQPVSARNLPDLKTISDQVDCAI
HCCCEEEEEECCCCCCHHHEEEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHCCCCEEE
IADEACFSPEDALNIAKINACDGVNIKLMKSGGIEKAQSIYHIAQAAKMQIMVGCMLESP
EECCCCCCCHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEHHCCC
IGVAAIASFALSKPDISYADLDPIFLIRENYILGGAQSLSNKIILAEKPGLGIEGITQGL
CCHHHHHHHHCCCCCCCCCCCCEEEEEECCEEECCHHHHCCEEEEEECCCCCHHHHHCCC
NLIGVIR
CEEEEEC
>Mature Secondary Structure
MNIKEIHIAELTIPLSRPFITAVRRTECVDDVVILIKTDCGKIGYGSAASTPAITGDSKE
CCCCEEEEEEEEEECCCHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
SIIAAIKNALGPPLIGRDISAFNLLLEMNDRAIGGNSSAKAAIDIALHDLFAQYCGLPLY
HHHHHHHHHCCCCCCCCCHHHEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCHHH
KQLGGNTNCINSCITISVKEVEEMVGDAIDLIQRGHKTLKIKLGLNPVDDINRIRAIRQA
HHHCCCCHHCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHH
VGNDITLLADANQGWSYEEALIAITAFKKENLNVPLVEQPVSARNLPDLKTISDQVDCAI
HCCCEEEEEECCCCCCHHHEEEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHCCCCEEE
IADEACFSPEDALNIAKINACDGVNIKLMKSGGIEKAQSIYHIAQAAKMQIMVGCMLESP
EECCCCCCCHHCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEEEHHCCC
IGVAAIASFALSKPDISYADLDPIFLIRENYILGGAQSLSNKIILAEKPGLGIEGITQGL
CCHHHHHHHHCCCCCCCCCCCCEEEEEECCEEECCHHHHCCEEEEEECCCCCHHHHHCCC
NLIGVIR
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]