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The map label for this gene is icfA [H]

Identifier: 148359489

GI number: 148359489

Start: 2256493

End: 2257542

Strand: Reverse

Name: icfA [H]

Synonym: LPC_1398

Alternate gene names: 148359489

Gene position: 2257542-2256493 (Counterclockwise)

Preceding gene: 148359491

Following gene: 148359488

Centisome position: 63.12

GC content: 36.29

Gene sequence:

>1050_bases
ATGTTAATTAAACTGATGCTCGGATTACTTGAATTTAAAAAAAAACCTTTTATTGGGATGAGAGATTTGTTTGAGCAATT
AAGCACCGGGCAGAATCCAGAAACACTATTTATAACGTGCTCTGACTCAAGGATTGTTCCGAATCTTATAACACAAGCAG
ATCCAGGTGATTTATTCAGCATACGTAATATAGGCAATATCATTCCTCCTTATCCATCAAGCTATAGTGAAGCCGGAGCT
ATTGAGTACGCTTTAAAAGTATTAGAGATAAAAGACATTATCATTTGTGGCCACTCCAATTGTGGTGCAATGCAAGGATT
ATTAACTCCGCATATTGAGGAGCATTTGCCGGGCGTAGCATCGTGGCTAAATCACTCTCAAGCTGTGTTACAAGAGGTGC
ATGAGGAACGAGCAACTGAAACTAATACTCCCGCTCGAGAACTTGAGATTGCTACCAAGAAGAATATTCTGCTCCAAATG
GAACATTTGAAAACCTATCCACTGGTCATCGAAAAAACAGCTCATAAGGAGTTAACAATTCATGGTTGGTATTATGAACT
GGAAACTGGAAAGGTATTTATTTATGAACCCGATTTAAAAGAATTTATTAATCTCGAATATGCATTAGACCGGGCTCTTG
AGTCCAGAAAAAACAAAATTGTGTCTGGTCTTTGTATGAATTACCTTCAACAAATGATTCCATTAAAATCAGAAAAGGAA
TATTACCGGTTAATGGAATTAATTACTAATCTTCAAGAAGATATTCTGTCAATTTGGGAATTTATTAGAGAACCAGCCAA
ACAGCAATTATGGAAGGAGTTAGGGGGCTTTTATGCCAGTCCGGAAGACGAAGAATTTATAGCCATGGTAGAATCAAGTA
CATCAATAAAACTCACTGAATTAGAAGAGTTTAAGAAAAAGATTTTCTTTGCCAATAATAATACCGCGACAAGCCTATTA
CTCAACTCATCAATTTTTAAAAAACCAACAAAGGATATTGGTAAACTAAATGCTGAGCCAGTCATAAGTTGTAACCCTCC
CCTAAAATAG

Upstream 100 bases:

>100_bases
ACATTTGTTTACGATATTGCAACATTGCTTTACGATTCTTTGTTGTTATGATAATTTGCCTTCATATGATGGATTCTGTA
AAGAAATTATGAGGTTGATT

Downstream 100 bases:

>100_bases
GTGCATTTCTGATTAGAGTTCTCCAAGTTAAACTATGTTTGAGGAGAACAAAAATGAAAAAATCGTGTTATACAGAAACA
CAAATTGTAAAAATTCTAAA

Product: (beta)-carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSRIVPNLITQADPGDLFSIRNIGNIIPPYPSSYSEAGA
IEYALKVLEIKDIIICGHSNCGAMQGLLTPHIEEHLPGVASWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQM
EHLKTYPLVIEKTAHKELTIHGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKNKIVSGLCMNYLQQMIPLKSEKE
YYRLMELITNLQEDILSIWEFIREPAKQQLWKELGGFYASPEDEEFIAMVESSTSIKLTELEEFKKKIFFANNNTATSLL
LNSSIFKKPTKDIGKLNAEPVISCNPPLK

Sequences:

>Translated_349_residues
MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSRIVPNLITQADPGDLFSIRNIGNIIPPYPSSYSEAGA
IEYALKVLEIKDIIICGHSNCGAMQGLLTPHIEEHLPGVASWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQM
EHLKTYPLVIEKTAHKELTIHGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKNKIVSGLCMNYLQQMIPLKSEKE
YYRLMELITNLQEDILSIWEFIREPAKQQLWKELGGFYASPEDEEFIAMVESSTSIKLTELEEFKKKIFFANNNTATSLL
LNSSIFKKPTKDIGKLNAEPVISCNPPLK
>Mature_349_residues
MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSRIVPNLITQADPGDLFSIRNIGNIIPPYPSSYSEAGA
IEYALKVLEIKDIIICGHSNCGAMQGLLTPHIEEHLPGVASWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQM
EHLKTYPLVIEKTAHKELTIHGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKNKIVSGLCMNYLQQMIPLKSEKE
YYRLMELITNLQEDILSIWEFIREPAKQQLWKELGGFYASPEDEEFIAMVESSTSIKLTELEEFKKKIFFANNNTATSLL
LNSSIFKKPTKDIGKLNAEPVISCNPPLK

Specific function: Reversible hydration of carbon dioxide. Essential to photosynthetic carbon dioxide fixation [H]

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm. Note=Associated with carboxysomes [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=199, Percent_Identity=39.1959798994975, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1786318, Length=182, Percent_Identity=32.4175824175824, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=25.3164556962025, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115532990, Length=239, Percent_Identity=25.9414225941423, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=167, Percent_Identity=30.5389221556886, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645213, Length=246, Percent_Identity=26.0162601626016, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 39869; Mature: 39869

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSRIVPNLITQADPGDLFS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEE
IRNIGNIIPPYPSSYSEAGAIEYALKVLEIKDIIICGHSNCGAMQGLLTPHIEEHLPGVA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHCCCCHHHHCCHHH
SWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQMEHLKTYPLVIEKTAHKELTI
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHEEHHHHCCCCEEEEECCCCEEEE
HGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKNKIVSGLCMNYLQQMIPLKSEKE
EEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YYRLMELITNLQEDILSIWEFIREPAKQQLWKELGGFYASPEDEEFIAMVESSTSIKLTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEHHH
LEEFKKKIFFANNNTATSLLLNSSIFKKPTKDIGKLNAEPVISCNPPLK
HHHHHHHEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MLIKLMLGLLEFKKKPFIGMRDLFEQLSTGQNPETLFITCSDSRIVPNLITQADPGDLFS
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEE
IRNIGNIIPPYPSSYSEAGAIEYALKVLEIKDIIICGHSNCGAMQGLLTPHIEEHLPGVA
HHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHCCCCHHHHCCHHH
SWLNHSQAVLQEVHEERATETNTPARELEIATKKNILLQMEHLKTYPLVIEKTAHKELTI
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHEEHHHHCCCCEEEEECCCCEEEE
HGWYYELETGKVFIYEPDLKEFINLEYALDRALESRKNKIVSGLCMNYLQQMIPLKSEKE
EEEEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YYRLMELITNLQEDILSIWEFIREPAKQQLWKELGGFYASPEDEEFIAMVESSTSIKLTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEHHH
LEEFKKKIFFANNNTATSLLLNSSIFKKPTKDIGKLNAEPVISCNPPLK
HHHHHHHEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]