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The map label for this gene is gloA [H]

Identifier: 148359422

GI number: 148359422

Start: 2186878

End: 2187318

Strand: Reverse

Name: gloA [H]

Synonym: LPC_1329

Alternate gene names: 148359422

Gene position: 2187318-2186878 (Counterclockwise)

Preceding gene: 148359426

Following gene: 148359421

Centisome position: 61.16

GC content: 37.41

Gene sequence:

>441_bases
ATGAAATATTTACATACCATGGTTAGAGTTTCGAATTTAGAACAATCACTCGATTTTTATTGTAATAAATTGGGTTTGAT
AGAAGTAAAACGAACTGAAAATGCCAAAGGCCGTTTTACCTTGGTATTTTTAGCTGCTCCTGCTGATATAGAGCAAGTTC
AAACTTCTTATGGACCAATGCTAGAGTTGACTTATAACTGGGATCCGGAGGATTACACTGAGGGAAGAAATTTTGGCCAT
TTGGCATATCATGTAGATGATATCTATAAAATCTGCCAACATTTACAGGAATGTGGTGTCGTAATTAATAGGCCACCCAG
AGATGGCCACATGGCTTTTATTCGTTCTCCTGATAATATTTCTATCGAATTATTGCAAAAGGGTGAGCCTTTGCCAAAGC
AGGAACCTTGGTTATCTGCACCCAATATTGGACATTGGTAA

Upstream 100 bases:

>100_bases
ACAAAAGAGAATTAACAAAGCCTATTTGCTACCACATGATGGTTATTTAAACTAAGTTTCTGATTAATAAAAAATAATTT
TCTATAGTTAAGGATAATAG

Downstream 100 bases:

>100_bases
CCGGACAATTTCATTCTGGTAGTGAATAGTGGAAATAATTTAATCATACTAAAATAGTATTTCGGGCCAAATGGCAATGT
TGAATCAATCGATGCAAATT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGH
LAYHVDDIYKICQHLQECGVVINRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW

Sequences:

>Translated_146_residues
MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGH
LAYHVDDIYKICQHLQECGVVINRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW
>Mature_146_residues
MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADIEQVQTSYGPMLELTYNWDPEDYTEGRNFGH
LAYHVDDIYKICQHLQECGVVINRPPRDGHMAFIRSPDNISIELLQKGEPLPKQEPWLSAPNIGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=36.6197183098592, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=34.0277777777778, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16873; Mature: 16873

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADIEQVQTSYGPM
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHCCCCE
LELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVINRPPRDGHMAFIRSPDNI
EEEEECCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCC
SIELLQKGEPLPKQEPWLSAPNIGHW
EEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKYLHTMVRVSNLEQSLDFYCNKLGLIEVKRTENAKGRFTLVFLAAPADIEQVQTSYGPM
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHCCCCE
LELTYNWDPEDYTEGRNFGHLAYHVDDIYKICQHLQECGVVINRPPRDGHMAFIRSPDNI
EEEEECCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCC
SIELLQKGEPLPKQEPWLSAPNIGHW
EEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]