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The map label for this gene is lepA

Identifier: 148359413

GI number: 148359413

Start: 2172835

End: 2174667

Strand: Reverse

Name: lepA

Synonym: LPC_1317

Alternate gene names: 148359413

Gene position: 2174667-2172835 (Counterclockwise)

Preceding gene: 148359417

Following gene: 148359412

Centisome position: 60.8

GC content: 40.15

Gene sequence:

>1833_bases
ATGCTATTTGCAAGAAGGTTAGAACAGTTGAAAGATTTAAACCGAATTCGAAATTTTTCAATAATTGCCCATATTGATCA
TGGCAAGTCTACGTTGGCTGATCGATTTATCCAAATTTGTGGCGGGTTGACTGAGCGTGAAATGAGCTCGCAAGTTCTTG
ATTCTATGGATATTGAACGTGAGCGAGGTATTACCATCAAAGCGCAATGTGTTTCCTTGAATTACACAGCTAAAGATGGA
AAGACCTATTTATTGAATTTTATCGATACGCCAGGGCATGTTGATTTTAGTTACGAGGTATCTCGTTCTCTTGCCGCTTG
TGAAGGTGCTATTCTGGTTGTTGATGCAGCACAGGGAGTAGAAGCGCAAACTTTAGCAGTTTGTTATACAGCAATTGATC
AGTCTCTTACCGTATTGCCAGTACTGAATAAAATTGATTTACCGCAGGCAGAACCTGAACGGGTTATTTCCGAGATCGAA
GATATTATTGGACTCGATGCTCAAGATGCGATTAGAGTGAGTGCTAAAAGCGGCTTGGGTGTTAATGATGTTTTAGAGGC
ATTAGTTGCGAATATTCCCCCTCCGAAAGGCGATGTTCATGCCCCATTACAAGCACTTATTATTGACTCCTGGTTTGATA
GTTATTTAGGAGTTGTCTCACTGGTTCGTATTGTTAATGGCGCCATACGAAAAGGGGATAAAATGCGAGTGATGTCCACA
GGCAGGGCTTATGAAGTGGATCAAGTTGGTATTTTTACCCCCAAACGGACTAAACTGGATGCTTTATATGCTGGTGAAGT
GGGTTATGTTGTTGCTGGTATAAAGGAAATTCAGGGCGCTCCAGTTGGTGATACTTTAACTCTGGATAGAAATCCAGCTG
ATAAAGTTCTTCCAGGGTTTCAACGCGTTAAGCCCCAAGTTTATGCTGGTTTGTTTCCAGTCAGCTCGGATGATTTTGAG
GCATTTAGAGAGGCTTTGGCTAAATTGAGTTTAAATGATGCTTCTTTATTTTATGAGCCGGAATCTTCAGAGGCTTTGGG
ATTTGGTTTTCGTTGCGGATTTTTAGGCATGCTGCATATGGAAATCATTCAGGAGAGATTGGAAAGAGAATATAACCTTG
ATTTGATTTCTACGGCACCTACTGTTGTTTATCAGATTGTAACTCAGAAAGGGGAAACTTTATTAATTGATAATCCTTCG
CATTTGCCCCCAACCCCGCAAATAAAAGAGATGTATGAGCCAATTGTCAGAGCAAATATTCTAGTGCCACAAGATTATTT
GGGGCCTATCATTACCTTATGTGTTGAAAGACGTGGTGTTCAAGTCAGTATGACCTATAGTGGTCGTCATGTTTCTGTTG
TTTATGATATTCCCATGAGCGAAGTAGTCTCTGATTTCTTTGATAGATTAAAATCAGTTAGTAGAGGTTATGCCTCTTTG
GATTATAATTTCCAAAGATTCCAGATTGCTGATTTGGTCAAGATGGATATATTAATAAATAGCGAACGTGTTGATGCTCT
TGCTGTGATTGTTCATAGAGATTCCGCTCATAGCCGCGGAAAATTAATTGCGGAGAAGATGCAACAGCTTATTCCAAGAC
AAATGTTTGATGTGGCTATTCAGGCTGCTATAGGAAGCCATATTATTGCTAGGCAAACAGTGAAAGCATTGCGCAAAAAC
GTTACAGCAAAGTGTTATGGTGGTGATGTGACGCGTAAGCGGAAATTGCTGGAAAAACAAAAAGCTGGCAAGAAAAGAAT
GAAACAGGTGGGGCATGTAGAAATACCTCAAGAAGCATTTATGGCCGTGTTTCAAACAGATAAAAAAAAGTAG

Upstream 100 bases:

>100_bases
AAATTGGTAAATGAATAAGCAATTTTCATTCTCTTAAGAATATAAAAACGACTGAATTTGCTTTGCCTACTTTCTGCACG
CGAACATTTAAGATAGACTT

Downstream 100 bases:

>100_bases
AGCCGCTCATTTACTAATAAGGATGTGTTGACCTTTTCGTCAGCATCTGGACATAAGTCAAATCATGATTAAATTGTCTT
TTGCTTAAAATTGAGGATAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGAIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK

Sequences:

>Translated_610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGAIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK
>Mature_610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTAKDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGAIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=49.2586490939045, Blast_Score=626, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=42.4050632911392, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=41.958041958042, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306283, Length=135, Percent_Identity=45.1851851851852, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=44.4444444444444, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923640, Length=135, Percent_Identity=45.1851851851852, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=37.1069182389937, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=43.4782608695652, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=43.4782608695652, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=43.4782608695652, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=38.8059701492537, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=38.8059701492537, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=30.9623430962343, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=30.9623430962343, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI94966752, Length=94, Percent_Identity=43.6170212765958, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=72.1476510067114, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=509, Percent_Identity=27.7013752455796, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=34.5911949685535, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=31.4465408805031, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=254, Percent_Identity=28.3464566929134, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=40.1960784313725, Blast_Score=487, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=465, Percent_Identity=25.1612903225806, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17533571, Length=164, Percent_Identity=32.3170731707317, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=37.4193548387097, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=171, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=223, Percent_Identity=29.5964125560538, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=609, Percent_Identity=45.8128078817734, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324707, Length=162, Percent_Identity=38.2716049382716, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=162, Percent_Identity=38.2716049382716, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=36.6666666666667, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=137, Percent_Identity=36.4963503649635, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=197, Percent_Identity=35.0253807106599, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=286, Percent_Identity=27.6223776223776, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=30.4964539007092, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.1860465116279, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24582462, Length=168, Percent_Identity=38.0952380952381, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28574573, Length=152, Percent_Identity=42.7631578947368, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.7350993377483, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=38.0952380952381, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=38.0952380952381, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=38.0952380952381, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=36.2962962962963, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28572034, Length=233, Percent_Identity=28.3261802575107, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_LEGPA (Q5X443)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124155.1
- ProteinModelPortal:   Q5X443
- SMR:   Q5X443
- STRING:   Q5X443
- GeneID:   3117949
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1837
- LegioList:   lpp1837
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   LPNE297246:LPP1837-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67756; Mature: 67756

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIER
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGV
HCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCC
EAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIEDIIGLDAQDAIRVSAKSGLG
CHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCC
VNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGAIRKGDKMRVMST
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGF
CCEEEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCH
QRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHM
HHHCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
EIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPSHLPPTPQIKEMYEPIVRANI
HHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCEE
LVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
ECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEC
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAI
CCCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
QAAIGSHIIARQTVKALRKNVTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAF
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
MAVFQTDKKK
HHHHHHCCCC
>Mature Secondary Structure
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIER
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ERGITIKAQCVSLNYTAKDGKTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGV
HCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCC
EAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIEDIIGLDAQDAIRVSAKSGLG
CHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCC
VNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGAIRKGDKMRVMST
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGF
CCEEEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCH
QRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHM
HHHCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
EIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPSHLPPTPQIKEMYEPIVRANI
HHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCEE
LVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
ECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEC
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAI
CCCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
QAAIGSHIIARQTVKALRKNVTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAF
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
MAVFQTDKKK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA