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The map label for this gene is tig

Identifier: 148359407

GI number: 148359407

Start: 2165428

End: 2166807

Strand: Reverse

Name: tig

Synonym: LPC_1307

Alternate gene names: 148359407

Gene position: 2166807-2165428 (Counterclockwise)

Preceding gene: 148359410

Following gene: 148359406

Centisome position: 60.59

GC content: 38.91

Gene sequence:

>1380_bases
ATGCAAGTTTCTGTTGAGACCCTAGAAGGTTTGGAGCGTAAGGTTACAGTTTCTGTACCTACAGAGAAAGTTGAGGAAGA
GGTGAGCTCGCGTCTCAGAAATCTTGCTCGCAAAGTGAAAATAGATGGTTTTCGTCCAGGCAAAGTCCCTTTTAATGTTG
TAAAAAGCCGTTTCTCTGATAGTGTGCGTGAAGAAGTTGCCCGGGAAATGGTACAATCTACCTTATATGAAGCCTTACAA
AAAAATGAATTGGTGCCAGCAGGTTATCCTCATGTTGAGCCTTTAGAAATCGAACCAGGTAAGGATTTTAAATACACTGC
TGTATTTGAAGTTATGCCCGTTTTTGAAATTGTCGAACTCAATCAAGCTCCAGTGGAATTAATTCAATCTGAAGTCACTG
ATAAAGATGTTGACAACATGATTGAGAAATTACGAGAACAAAACAAAGAGTGGCATGAAGTAACTCATGCGGTCAAAAAA
GGTGATAAAGTTGTTATTGATTTTCAGGGATTTTTGGATGACAAGCCATTCCAGGGCGGCAGTGCTGAAGGATATGAGCT
TGTGATTGGTTCTGGATCGATGATTCCCGGATTTGAAGATGGAATTGTTGGTGGGAAAATTGACAAACCGTTCGATATAA
AAGTGAGTTTCCCGGAAGATTATGGCCATAAAGATTTGGCTGGGAAGGAAGCAACGTTCAAGATCACTATCAAAAAGATT
ATGGAAGGCAAGTTGCCTGCTTTGGATGAGGCATTTGCGGAGAAATTTAATATAAAAGAAGGTGGTATAGAGTCTCTTAA
AAAGGATATTAGAGAAAATATGGCGCGTGAATTGGAACGTCGCGTCAACATGATGAACAGAGAGAAACTGTTTGATTCAT
TAATGTCTGTGAATCATGTTGAGTTGCCAATAGCTTTGATAGATAAAGAAATTGAGCATTTAAAGCATGATATGTATCAC
CGATTGTTTGGTCACGAGCATAAAGACGATGAAAAAATTCCTGATTTCCCAAGGGAGCTATTTGAAGAACAAGCCAAGCG
ACGTGTACACCTGGGCTTACTTTTTGCTGAGTATGTAAAAAAACACGAAATTGTTGCAGACAACGACAAAGTTAATGCTA
TGATTGATAAATTTGCCAGTGCTTACGAAAGTCCTGATGAGCTTCGCGCGTGGTATCAAAGCAGCAAAGAACATATGGCT
GAAGTTGAAGCATTAGTTATGGAAGATATGGTTGCTGATAAAATAGCAGAAGATGCGAAACTTAAATATAAAAATATGGA
TTATGATTCAGTGATGAATCCCAAAAAGGGCACTGAGAAAAAAGGAGAGTTAGCATGCCAGGCTATTCAGATTATATTAT
CCGTAATGCAAGCGGATTAA

Upstream 100 bases:

>100_bases
ATTGGTAGACGCACTGGATTTAGGTTCCAGCGGGGCGACCCGTGAGAGTTCGAGTCTCTCCTTTCGCACCATTTCATGAT
AACTTCCGAGAGGTGAATTA

Downstream 100 bases:

>100_bases
TACCAATGGTTATTGAGCAAACCTCGCGAGGTGAGCGTTCCTATGATATTTACTCAAGATTATTAAAAGAAAGAATTATT
TTTCTCTTGGGTGAAGTAGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGFRPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQ
KNELVPAGYPHVEPLEIEPGKDFKYTAVFEVMPVFEIVELNQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFPEDYGHKDLAGKEATFKITIKKI
MEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMARELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYH
RLFGHEHKDDEKIPDFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWYQSSKEHMA
EVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGELACQAIQIILSVMQAD

Sequences:

>Translated_459_residues
MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGFRPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQ
KNELVPAGYPHVEPLEIEPGKDFKYTAVFEVMPVFEIVELNQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFPEDYGHKDLAGKEATFKITIKKI
MEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMARELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYH
RLFGHEHKDDEKIPDFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWYQSSKEHMA
EVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGELACQAIQIILSVMQAD
>Mature_459_residues
MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGFRPGKVPFNVVKSRFSDSVREEVAREMVQSTLYEALQ
KNELVPAGYPHVEPLEIEPGKDFKYTAVFEVMPVFEIVELNQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKK
GDKVVIDFQGFLDDKPFQGGSAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFPEDYGHKDLAGKEATFKITIKKI
MEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMARELERRVNMMNREKLFDSLMSVNHVELPIALIDKEIEHLKHDMYH
RLFGHEHKDDEKIPDFPRELFEEQAKRRVHLGLLFAEYVKKHEIVADNDKVNAMIDKFASAYESPDELRAWYQSSKEHMA
EVEALVMEDMVADKIAEDAKLKYKNMDYDSVMNPKKGTEKKGELACQAIQIILSVMQAD

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=433, Percent_Identity=41.3394919168591, Blast_Score=344, Evalue=8e-96,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_LEGPC (A5ID18)

Other databases:

- EMBL:   CP000675
- RefSeq:   YP_001250614.1
- ProteinModelPortal:   A5ID18
- SMR:   A5ID18
- STRING:   A5ID18
- GeneID:   5181127
- GenomeReviews:   CP000675_GR
- KEGG:   lpc:LPC_1307
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   LPNE400673:LPC_1307-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 52463; Mature: 52463

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGFRPGKVPFNVVKSRFSD
CCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKDFKYTAVFEVMPVFEIVEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
NQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKKGDKVVIDFQGFLDDKPFQGG
CHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
SAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFPEDYGHKDLAGKEATFKITIKKI
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHH
MEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMARELERRVNMMNREKLFDSLMSVNHV
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIPDFPRELFEEQAKRRVHLGLLFAEYVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KHEIVADNDKVNAMIDKFASAYESPDELRAWYQSSKEHMAEVEALVMEDMVADKIAEDAK
HCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKYKNMDYDSVMNPKKGTEKKGELACQAIQIILSVMQAD
HHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVSVETLEGLERKVTVSVPTEKVEEEVSSRLRNLARKVKIDGFRPGKVPFNVVKSRFSD
CCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
SVREEVAREMVQSTLYEALQKNELVPAGYPHVEPLEIEPGKDFKYTAVFEVMPVFEIVEL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
NQAPVELIQSEVTDKDVDNMIEKLREQNKEWHEVTHAVKKGDKVVIDFQGFLDDKPFQGG
CHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
SAEGYELVIGSGSMIPGFEDGIVGGKIDKPFDIKVSFPEDYGHKDLAGKEATFKITIKKI
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHH
MEGKLPALDEAFAEKFNIKEGGIESLKKDIRENMARELERRVNMMNREKLFDSLMSVNHV
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELPIALIDKEIEHLKHDMYHRLFGHEHKDDEKIPDFPRELFEEQAKRRVHLGLLFAEYVK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KHEIVADNDKVNAMIDKFASAYESPDELRAWYQSSKEHMAEVEALVMEDMVADKIAEDAK
HCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKYKNMDYDSVMNPKKGTEKKGELACQAIQIILSVMQAD
HHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA