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The map label for this gene is mpl [H]

Identifier: 148359297

GI number: 148359297

Start: 2036060

End: 2037385

Strand: Reverse

Name: mpl [H]

Synonym: LPC_1194

Alternate gene names: 148359297

Gene position: 2037385-2036060 (Counterclockwise)

Preceding gene: 148359298

Following gene: 148359296

Centisome position: 56.97

GC content: 41.55

Gene sequence:

>1326_bases
ATGAGTGCCTTGGCTTTGCTCGCCCGCGACGCAGGATATAGGGTGACAGGCAGCGATGCCAATTGCTATCCGCCTATCAG
TGATTTGCTTGCTGCCAAAGGTATTTCATGGACTGAAGGCTACGATGATTCCACTTTAGCATTGCAATCTGACATGGTTA
TTGTCGGTAATGCTATTAAGCGGGGAATGCCGGTTCTTGAAGCAGTTATGGAGTCGGGAAAACCATATATGTCAGGCCCT
CAATGGCTTGCCGAAAATATTTTATCAAAATATCAGGTGATCGCGGTAGCTGGAACTCATGGCAAAACGACTACCACTTC
AATGATTGCCTGGATTTTGGATCAAGCTGGAATGAATCCGGGATTTCTCATTGGGGGCGTCTCTAGTGATTTCAATACCA
GCGCCTGCCTTGGCAAAGGGAGATGGTTTGTCATAGAGGCAGATGAGTACGATAGTGCTTTTTTTGATAAACGACCTAAA
TTTATGCATTATCGACCAAATATTGCAGTATTAAACAATTTGGAATTTGATCATGCCGATATTTATCCGGACTTGGCAGC
TATTCAACAACAATTTCATTATTTATTAAGAACAATTCCCAAAAGTGGTGTCGCCATCAAGCCTCGAGATGATAAGGCTC
TGAATGAAGTTCTATCACGAGGCCAATTTTCGCGTATTGAAACACTGGCACTATCTGGAGACGCCGAATGGGTTGCCGAA
CTGATTGACGATAGTGGTTCACAATTTAAAATACTCCATAATGGGGTAATGGTTGCTGAAGTATCTTGGCCGTTGATAGG
GCAATTCAATGTAGAAAATGGTTTGGCTGCAATTGCTGCTACCTGGAATGCTGGAGTAAATCCTGTGGTTGCAGCCAAGG
CTCTAGAACAGTTTTCTCCAGTGAAACGAAGGCTTGAAGTTCGTTCCAATCGACATGGAGTAACCGTTTATGATGATTTT
GCCCACCATCCAACCGCAATTACTAAAACTATTGATGCTTTAAAAAGAAGTGGACGACATCAACGTATTTTTGCTGTTAT
GGAGTTTGCTTCATATACTATGAGAACGGGTGTACATGCCGGAGAAATGGCTCATGCTTTAGCTCCTGTTGATGGTGCCT
ATGTCCTGGAGCCTCAGGATTTTAATTTAAAAGATTTTGTTAGCTCCTGGATTTGCCCGTATAAAATTTGCAAAAACAAT
GGAGAGATAGTTAAAGAAATTATCAAAACAGTAAGAGAGGGCGATGCTGTATTGGTAATGAGTAATAGAGGATTTAATGG
AATTCATCAACAATTGATCGATTCTATAGATAAACTTTTTATGTAA

Upstream 100 bases:

>100_bases
TTAGCTGCTTTTGGTTTAGTCGCTGTTAACATTTTAACTATTTCTTTTTGGCGATGACATGATGACACATTTGCATATAC
TAGGAGTAAGTGGAACCTTT

Downstream 100 bases:

>100_bases
CGATTCATTTTTATTCTGAAATGTCTTGAGAACATATAATGATATTGAGAAGCAGGTGAAATAAAAGTCATGTCCTATTA
TTTGCAAAGCCCCAACACAA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGP
QWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPK
FMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAE
LIDDSGSQFKILHNGVMVAEVSWPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGVTVYDDF
AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEPQDFNLKDFVSSWICPYKICKNN
GEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM

Sequences:

>Translated_441_residues
MSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGP
QWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPK
FMHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAE
LIDDSGSQFKILHNGVMVAEVSWPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGVTVYDDF
AHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEPQDFNLKDFVSSWICPYKICKNN
GEIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM
>Mature_440_residues
SALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIKRGMPVLEAVMESGKPYMSGPQ
WLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNPGFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPKF
MHYRPNIAVLNNLEFDHADIYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAEL
IDDSGSQFKILHNGVMVAEVSWPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSPVKRRLEVRSNRHGVTVYDDFA
HHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHAGEMAHALAPVDGAYVLEPQDFNLKDFVSSWICPYKICKNNG
EIVKEIIKTVREGDAVLVMSNRGFNGIHQQLIDSIDKLFM

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=441, Percent_Identity=48.9795918367347, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1786279, Length=385, Percent_Identity=29.8701298701299, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48560; Mature: 48429

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIK
CCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCEEEECCHHH
RGMPVLEAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNP
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
GFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHAD
CEEEECCCCCCCCHHEECCCEEEEEECCCCCCHHHCCCCCCEEECCCEEEEECCCCCCHH
IYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAE
CCHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHH
LIDDSGSQFKILHNGVMVAEVSWPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSP
HHCCCCCEEEEEECCEEEEEECCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
VKRRLEVRSNRHGVTVYDDFAHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHA
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
GEMAHALAPVDGAYVLEPQDFNLKDFVSSWICPYKICKNNGEIVKEIIKTVREGDAVLVM
HHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEE
SNRGFNGIHQQLIDSIDKLFM
ECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SALALLARDAGYRVTGSDANCYPPISDLLAAKGISWTEGYDDSTLALQSDMVIVGNAIK
CHHHHHHCCCCCEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCEEEECCHHH
RGMPVLEAVMESGKPYMSGPQWLAENILSKYQVIAVAGTHGKTTTTSMIAWILDQAGMNP
HCCHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHCCCCC
GFLIGGVSSDFNTSACLGKGRWFVIEADEYDSAFFDKRPKFMHYRPNIAVLNNLEFDHAD
CEEEECCCCCCCCHHEECCCEEEEEECCCCCCHHHCCCCCCEEECCCEEEEECCCCCCHH
IYPDLAAIQQQFHYLLRTIPKSGVAIKPRDDKALNEVLSRGQFSRIETLALSGDAEWVAE
CCHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHH
LIDDSGSQFKILHNGVMVAEVSWPLIGQFNVENGLAAIAATWNAGVNPVVAAKALEQFSP
HHCCCCCEEEEEECCEEEEEECCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHH
VKRRLEVRSNRHGVTVYDDFAHHPTAITKTIDALKRSGRHQRIFAVMEFASYTMRTGVHA
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
GEMAHALAPVDGAYVLEPQDFNLKDFVSSWICPYKICKNNGEIVKEIIKTVREGDAVLVM
HHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEE
SNRGFNGIHQQLIDSIDKLFM
ECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]