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The map label for this gene is iscS [H]

Identifier: 148359290

GI number: 148359290

Start: 2027156

End: 2028319

Strand: Reverse

Name: iscS [H]

Synonym: LPC_1186

Alternate gene names: 148359290

Gene position: 2028319-2027156 (Counterclockwise)

Preceding gene: 148359291

Following gene: 148359289

Centisome position: 56.71

GC content: 41.49

Gene sequence:

>1164_bases
TTGGCAATAAAACCGATTTATTTTGATTATATGGCAACAACACCCGTTGATCCTCGCGTTGTTGAGCAAATGATAAAATA
TTTAGGCCCTGAAGGGGATTTTGGTAATCCTGCATCAGCAACTCATGAGTATGGGAGAGTTGCCTCCATGGCAGTTGAGC
AGGCACGCTCTCAAATTGCAGAAACTATTAATGCCTCACCCCAAGAAATAGTGTTTACATCAGGAGCTACAGAAGCGGAT
AATTTGGCGATTTTAGGAGCTGCCCGGTTTTATAAAAATAAGGGAATGCATCTGGTTACTATGAGCACTGAACACAAAGC
TGTGCTTGACAGTTTTCACCAACTGGAAAAAGAAGGTTTTCAAGTCACCTACCTTAATCCGGAATCAGATGGTTTGTTGG
ATCTTGGAAAACTGGAATCTGCTCTAAGGTTAGATACTATTCTGGTTTCTGTCATGCATGTTAATAATGAAATTGGTGTC
ATCCAAGATATTGCATCTATTGGTGAGCTATTACGAAATAGGGGAATTATATTTCATGTTGACGCGGCTCAAAGTGCTGG
TAAATTGCCAATCGATTTAAGCCAGCTTTCAGTTGATTTGATGTCATTCTCTGCTCATAAAAATTATGGGCCTAAAGGTG
TAGGGGCTCTGTATGTCAGGCATAAGCCAAGGATTCGTTTACAGCCTTTAAGTTACGGTGGCGGGCATGAGGGTGGATTG
CGCTCAGGAACATTACCTACCCATCAAATTGTCGGCATGGGTGAGGCTTTTGCTATTGCAGAGGCTGATAGAATTCCTGA
ACAGACGCGTATTCTAAATTTACGCAAACAACTATGGGACGGAATACGACATTTGCCTGCTATTAAACTGAATGGACATG
AGCACAGGCGTATCGCTGGAAATCTGAATGTGAGTTTTGTTGGATTAAATGGAGATTCATTGCTGTTTGCTTTAAGTGAG
TTAGCGATATCAACTACTTCTGCCTGTTCTTCTGCCAGTATTCAACCATCTTATGTCTTAAGGGCAATTGGTTTGACTGA
TACAGAGGCTCAATCAACTATTCGTTTATCCATAGGACGCTTCACTTCTGAGGTGCAAATAAAGAAGGCAATTGATATAA
TTTGCACACAAGTGAGTCGGCTGCACGAATTATCGCCGTTATGA

Upstream 100 bases:

>100_bases
ATCAACCTTGAAAAAATGGAAGTTAGTATTTTAAGAGGTATGCTTAGCCAGGTTCAGAAATCATTGGAGTGGGCAAGGAA
AGACAATAGGAGCAATAAAA

Downstream 100 bases:

>100_bases
TGTATAATAAAATTGTACAGGATTATTTTTTTCAGCCACAACATATTGGTAAGCTGGATTTAAATGATCCTTTTGTAATA
GGTGTACAAAGTCAACCGAT

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQARSQIAETINASPQEIVFTSGATEAD
NLAILGAARFYKNKGMHLVTMSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGV
IQDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQPLSYGGGHEGGL
RSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSE
LAISTTSACSSASIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL

Sequences:

>Translated_387_residues
MAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQARSQIAETINASPQEIVFTSGATEAD
NLAILGAARFYKNKGMHLVTMSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGV
IQDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQPLSYGGGHEGGL
RSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSE
LAISTTSACSSASIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL
>Mature_386_residues
AIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQARSQIAETINASPQEIVFTSGATEADN
LAILGAARFYKNKGMHLVTMSTEHKAVLDSFHQLEKEGFQVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGVI
QDIASIGELLRNRGIIFHVDAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQPLSYGGGHEGGLR
SGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLPAIKLNGHEHRRIAGNLNVSFVGLNGDSLLFALSEL
AISTTSACSSASIQPSYVLRAIGLTDTEAQSTIRLSIGRFTSEVQIKKAIDIICTQVSRLHELSPL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=50.5181347150259, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=30.3102625298329, Blast_Score=164, Evalue=9e-41,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=60.8355091383812, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI1787970, Length=285, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=24.4949494949495, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=50.5181347150259, Blast_Score=391, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=33.4355828220859, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=50, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=50.3896103896104, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42144; Mature: 42013

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQARSQIA
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVTMSTEHKAVLDSFHQLEKEGF
HHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
QVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGVIQDIASIGELLRNRGIIFHV
EEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEE
DAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQPLSYGGGHEGGL
ECCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
RSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLPAIKLNGHEHRRIAG
CCCCCCCHHHCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEC
NLNVSFVGLNGDSLLFALSELAISTTSACSSASIQPSYVLRAIGLTDTEAQSTIRLSIGR
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCCEEEEEECC
FTSEVQIKKAIDIICTQVSRLHELSPL
CCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIKPIYFDYMATTPVDPRVVEQMIKYLGPEGDFGNPASATHEYGRVASMAVEQARSQIA
CCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ETINASPQEIVFTSGATEADNLAILGAARFYKNKGMHLVTMSTEHKAVLDSFHQLEKEGF
HHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
QVTYLNPESDGLLDLGKLESALRLDTILVSVMHVNNEIGVIQDIASIGELLRNRGIIFHV
EEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEE
DAAQSAGKLPIDLSQLSVDLMSFSAHKNYGPKGVGALYVRHKPRIRLQPLSYGGGHEGGL
ECCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCC
RSGTLPTHQIVGMGEAFAIAEADRIPEQTRILNLRKQLWDGIRHLPAIKLNGHEHRRIAG
CCCCCCCHHHCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEC
NLNVSFVGLNGDSLLFALSELAISTTSACSSASIQPSYVLRAIGLTDTEAQSTIRLSIGR
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCCCCCEEEEEECC
FTSEVQIKKAIDIICTQVSRLHELSPL
CCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA