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The map label for this gene is pgk [H]

Identifier: 148358296

GI number: 148358296

Start: 173210

End: 174400

Strand: Reverse

Name: pgk [H]

Synonym: LPC_0158

Alternate gene names: 148358296

Gene position: 174400-173210 (Counterclockwise)

Preceding gene: 148358297

Following gene: 148358295

Centisome position: 4.88

GC content: 44.58

Gene sequence:

>1191_bases
ATGAACCTGATTAAAATGAGCGACATTGATCTGTCTGGCAAAAGAGTTCTGATTCGTGAGGATTTAAATGTCCCGATCAA
AGACGGCATGATTACCAGTGATCAAAGATTGCAAGCGGCCCTGCCTACAATCAAATCCGCTTTGGACAGTGGTGCAGCAG
TAATCGTATTGTCTCATCTTGGGCGTCCGGAAGAAGGCAAATACGAAAAAAAATTCTCTTTGGAACCTGTTGCTGATTAT
TTAAGGGAAAACCTGGAATATCCTGTTCGCTTCGTTAAAGATTACCTGACCGGCGTGGATGTAAACCCTGGGGAATTGGT
CGTATGCGAAAATGTCCGATTTAATCCCGGTGAAAAAGGCAATGACGAAGCTTTGGCAAAAAAACTGGCGAGCCTTTGTG
ATGTCTTTGTCATGGATGCTTTTGGCACAGCACACAGGGCACAAGCTTCTACCTATGGTGTCGCTCAATATGCTCCAGTT
GCCGTTGCTGGTCCTCTGTTAATCAGGGAACTTGAAGCGTTAAACCAGGTACTCAAAGCCCCCAAAAAACCTATCGTCGC
TATTGTTGGGGGAGCCAAAGTCTCATCCAAGCTCAGTTTATTGAAACAATTAGTGGGTATGGTCGATGTTCTTATCCCTG
GTGGAGGTATCGCCAATACTTTTCTGAAAGCCCAGGGATTTGAAATTGGTATTTCCCTTTACGAACCTGATTTATTGGAT
GAGGCTCGACATATCCTTATTCTGGCCAAAGAGAAAGGTTGCCAAATTCCTCTACCCACCGACGTTGTAGTGGGCAAAAC
ATTTAGTGAAACCTGCCCTGCTTTCAACAAATCTCTGTCTAATGTGGCTGCGGACGATATGATCCTTGATATTGGACCAG
AAACCATAAGAGATTATGTAGATCTCATCCATGAAGCCAATACTATCATCTGGAATGGTCCGGTTGGCGTATTCGAATTC
CCGCAGTTTGCTTACGGAACAAGAGCCATTGCCATAGCGATTGCTGAGAGTGATGCCTTTTCTATTGCTGGTGGTGGTGA
TACCTTGGCAGCTGTTGACCTTTATGACTTGAATCAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTGGAAT
GCCTGGAAGGAAAAACCTTGCCTGCTGTTGCTATTTTGCAGGAGCGCGCAAAACATGTTAAGACGAACTAA

Upstream 100 bases:

>100_bases
AATTTGGTCAAAGTAGTAGCCTGGTATGACAATGAATGGGGTTTCTCTAACCGCATGATTGATACCGCACACCAAATGAT
GAACTGCTAAAGGATAACCA

Downstream 100 bases:

>100_bases
GATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGCTGGAGTCAACGTAGTAC
GAATTAATTTTTCTCATGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLTGVDVNPGELVVCENVRFNPGEKGNDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Sequences:

>Translated_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLTGVDVNPGELVVCENVRFNPGEKGNDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
>Mature_396_residues
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHLGRPEEGKYEKKFSLEPVADY
LRENLEYPVRFVKDYLTGVDVNPGELVVCENVRFNPGEKGNDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPV
AVAGPLLIRELEALNQVLKAPKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYVDLIHEANTIIWNGPVGVFEF
PQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=40.0966183574879, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=39.3203883495146, Blast_Score=274, Evalue=8e-74,
Organism=Escherichia coli, GI1789294, Length=391, Percent_Identity=62.9156010230179, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=42.2705314009662, Blast_Score=297, Evalue=6e-81,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=40.3381642512077, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=41.9277108433735, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24581203, Length=413, Percent_Identity=40.4358353510896, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42552; Mature: 42552

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLTGVDVNPGELVVCENVRFNPGEKG
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
NDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVAGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQ
HHHHHCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCCEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLIKMSDIDLSGKRVLIREDLNVPIKDGMITSDQRLQAALPTIKSALDSGAAVIVLSHL
CCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
GRPEEGKYEKKFSLEPVADYLRENLEYPVRFVKDYLTGVDVNPGELVVCENVRFNPGEKG
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
NDEALAKKLASLCDVFVMDAFGTAHRAQASTYGVAQYAPVAVAGPLLIRELEALNQVLKA
CHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHC
PKKPIVAIVGGAKVSSKLSLLKQLVGMVDVLIPGGGIANTFLKAQGFEIGISLYEPDLLD
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHH
EARHILILAKEKGCQIPLPTDVVVGKTFSETCPAFNKSLSNVAADDMILDIGPETIRDYV
CCCEEEEEECCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCEEEECCHHHHHHHH
DLIHEANTIIWNGPVGVFEFPQFAYGTRAIAIAIAESDAFSIAGGGDTLAAVDLYDLNQQ
HHHHHCCEEEECCCCCCCCCCCHHCCCEEEEEEEECCCCEEEECCCCCEEEEEEECCCCC
ISYISTGGGAFLECLEGKTLPAVAILQERAKHVKTN
EEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA