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The map label for this gene is pykA [H]

Identifier: 148358295

GI number: 148358295

Start: 171802

End: 173226

Strand: Reverse

Name: pykA [H]

Synonym: LPC_0157

Alternate gene names: 148358295

Gene position: 173226-171802 (Counterclockwise)

Preceding gene: 148358296

Following gene: 148358294

Centisome position: 4.84

GC content: 42.32

Gene sequence:

>1425_bases
ATGTTAAGACGAACTAAGATTGTTGCCACTTTAGGCCCAGCCAGCAAGGAACCTGAAATTCTGCGATCCATGCTGGCTGC
TGGAGTCAACGTAGTACGAATTAATTTTTCTCATGCCGATTCCTCTGCCCTGCAATTAATTGCACTGGTTCGTAAAATCG
CAGATGAATTAAACCACCCGGTTGCAGTCATGGCTGACTTACAAGGGCCTAAAATAAGAGTCGGCCGTTTTCAAAACAAA
TCCATCACTTTAATTGATGGACAAAATTTTACATTGGATTGTATGGCTCCAGATACTCTGGGTGATATAAATGGCGTATC
AGTGGCTTATCCTAATTTGGCCAATGAACTCAGTATTAGTGATCATCTTCTGATTAATGATGGGTTAATTGAATTGGAAG
TCATAGAGATTTCCGGGTCAAAAATTCATTGCAAGGTAGTGGAAGGTGGAGTACTCACTGATTTGAAAGGTTTGAATCGA
AAAGGTGGCGGCTTAGCAGCCAGAACTCTAACAGAAAAAGATAGAAATGATTTACGTACAGCAATTGAAGCAGAAGTGGA
CTACATCAGTTTATCTTTTGTAAAAGACGCAGAGGATATCCGGCAAGCAAGAGCGCTAATGAAAGACTATGGGGCCCAAA
TAACCCCTATTATCGCTAAAATTGAGCGCATGGAAGCTTTGGATCACTTAACTGATATTATTCGCGAAGCAGACGCCATT
ATGGTGGCCAGAGGAGATTTAGGCGTTGAAGTGGGTGCTGCTGAAGTTCCCGCTATTCAAAAACACATCATAGAACAAAC
GCGACTTTTAGATAAAGTGGTGATTACCGCAACACAAATGATGGAATCCATGATAAGCAATCCTCAGCCTACCCGTGCCG
AGGTTTCTGATGTAGCCAACGCGATTCTGGATGGAACTGATGCTGTGATGCTTTCGGCTGAAACAGCAAGCGGCCTTTTT
CCAGTCAAAGTCATTACCATGGTCAATAAAATATGTTTAAGCGCTGAGAAACATGCCAGCTTTTTTTATCATAGTGATCT
GGAAACCTGTCATTATCAACGGGCTGACCAGGCAATCGCTATGGCTACCATGCATACCGCCAATCATTTTCCCATTCAGG
CCATTATCACTCTGACCGAATCCGGAGACACGGCTTTATGGGTATCCCGACACCACAGCACCGTTCCTATTTTTGCTATA
TCAGCCAATAAGAGAACCATTGGTCGTTTAAGCCTTGTAAACAATGTCTTTCCTATCTACATTGATTTTCATCAATTCAA
CCCTGAAGGATTGAATCAACAAATTTTACATGAATTAATCAAATCAGGGCACCTTGAAAAGAGAGGCTATGTTTTGTTAA
CTCGTGGAACCCAAATTGGAATGCCTGGCGGCACCAACTGCATGGAAATTATACCTGTTGTCTGA

Upstream 100 bases:

>100_bases
CAACAAATTTCTTATATTTCCACAGGAGGTGGGGCATTTCTGGAATGCCTGGAAGGAAAAACCTTGCCTGCTGTTGCTAT
TTTGCAGGAGCGCGCAAAAC

Downstream 100 bases:

>100_bases
GTTCAAGATATCCTGTTAATTAAAGCAAGGTTCTTTCATAAAAGACAAAAAATGATTATTTTAATGACACTTTAATCAAA
TTATCACCTCTATGCTACTA

Product: pyruvate kinase II

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSISDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDLETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV

Sequences:

>Translated_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSISDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDLETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
>Mature_474_residues
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHPVAVMADLQGPKIRVGRFQNK
SITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSISDHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNR
KGGGLAARTLTEKDRNDLRTAIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVANAILDGTDAVMLSAETASGLF
PVKVITMVNKICLSAEKHASFFYHSDLETCHYQRADQAIAMATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAI
SANKRTIGRLSLVNNVFPIYIDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.0887096774194, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.0887096774194, Blast_Score=258, Evalue=7e-69,
Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=34.0770791075051, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=34.0770791075051, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=33.6048879837067, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=34.6020761245675, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=34.6020761245675, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=31.6455696202532, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=31.6455696202532, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=31.0185185185185, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=45.0733752620545, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787965, Length=439, Percent_Identity=37.3576309794989, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=427, Percent_Identity=37.2365339578454, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=492, Percent_Identity=35.7723577235772, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=492, Percent_Identity=36.1788617886179, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=492, Percent_Identity=35.7723577235772, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=492, Percent_Identity=35.7723577235772, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=430, Percent_Identity=34.8837209302326, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=476, Percent_Identity=32.9831932773109, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI28571814, Length=434, Percent_Identity=36.1751152073733, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24648964, Length=434, Percent_Identity=36.1751152073733, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=36.231884057971, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=33.7995337995338, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24646914, Length=281, Percent_Identity=35.9430604982206, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51755; Mature: 51755

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIS
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEEECCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDLETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC
>Mature Secondary Structure
MLRRTKIVATLGPASKEPEILRSMLAAGVNVVRINFSHADSSALQLIALVRKIADELNHP
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
VAVMADLQGPKIRVGRFQNKSITLIDGQNFTLDCMAPDTLGDINGVSVAYPNLANELSIS
EEEEEECCCCEEEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCC
DHLLINDGLIELEVIEISGSKIHCKVVEGGVLTDLKGLNRKGGGLAARTLTEKDRNDLRT
CEEEEECCEEEEEEEEECCCEEEEEEECCCCEECHHCCCCCCCCEEEEHHHHHHHHHHHH
AIEAEVDYISLSFVKDAEDIRQARALMKDYGAQITPIIAKIERMEALDHLTDIIREADAI
HHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
MVARGDLGVEVGAAEVPAIQKHIIEQTRLLDKVVITATQMMESMISNPQPTRAEVSDVAN
EEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AILDGTDAVMLSAETASGLFPVKVITMVNKICLSAEKHASFFYHSDLETCHYQRADQAIA
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHH
MATMHTANHFPIQAIITLTESGDTALWVSRHHSTVPIFAISANKRTIGRLSLVNNVFPIY
HHHHHCCCCCCEEEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCEEEE
IDFHQFNPEGLNQQILHELIKSGHLEKRGYVLLTRGTQIGMPGGTNCMEIIPVV
EEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]