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The map label for this gene is dsbA [H]

Identifier: 148358281

GI number: 148358281

Start: 150000

End: 150614

Strand: Reverse

Name: dsbA [H]

Synonym: LPC_0143

Alternate gene names: 148358281

Gene position: 150614-150000 (Counterclockwise)

Preceding gene: 148358282

Following gene: 148358278

Centisome position: 4.21

GC content: 38.7

Gene sequence:

>615_bases
ATGTTTAAAAAATTAATTGCCCTATTATTTCTTATGCCAATGACTGCTTTGGCAACTCAATTTATTGAAGGCAAAGATTA
TCAAACCGTTGCCAGCGCGCAATTGTCTACCAATAAAGATAAAACACCCCTGATTACCGAATTTTTTAGCTATGGCTGTC
CATGGTGTTATAAAATTGATGCCCCACTAAATGATTGGGCAACCAGAATGGGGAAAGGAGCTCATCTTGAGCGCATTCCA
GTTGTTTTTAAACCAAACTGGGATTTGTATGCCAAAGCCTATTACACAGCAAAAACCCTGGCTATGTCCGATAAAATGAA
TCCCATATTATTCAAAGCAATTCAAGAAGATAAAAATCCACTGGCAACGAAACAATCGATGGTTGATTTTTTTGTTGCTC
ATGGCGTAGACAGAGAAATTGCCAAGAGCGCTTTTGAAAACTCACCAACAATCGATATGCGCGTTAACAGCGGCATGTCT
TTGATGGCGCATTATCAAATTAACGCAGTACCAGCCTTTGTCGTCAATAACAAGTACAAAACTGACTTACAAATGGCAGG
GAGCGAAGAGCGTTTATTTGAAATTTTGAATTACCTGGTAAGAAAATCAGCCTAA

Upstream 100 bases:

>100_bases
GACTTAAATCAAATTATGCAAGATATCAGTAGTAAAATGAGCCCAGAAGATATGGAAGCCGTTGCTTATTATATTCAAGG
GTTATATTGAGGAATTGACT

Downstream 100 bases:

>100_bases
TTAATTCAAGCTATAAAGAATATTCCTAATAGGTCAGTACCCTCCTGTTTCAATATTCTCTAACCGCCGGATTTGGTTGA
GGTGAGGGCTGAGCTCTTAC

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPLITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERIP
VVFKPNWDLYAKAYYTAKTLAMSDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA

Sequences:

>Translated_204_residues
MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPLITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERIP
VVFKPNWDLYAKAYYTAKTLAMSDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA
>Mature_204_residues
MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPLITEFFSYGCPWCYKIDAPLNDWATRMGKGAHLERIP
VVFKPNWDLYAKAYYTAKTLAMSDKMNPILFKAIQEDKNPLATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMS
LMAHYQINAVPAFVVNNKYKTDLQMAGSEERLFEILNYLVRKSA

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23174; Mature: 23174

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPLITEFFSYGCPWCYKID
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEC
APLNDWATRMGKGAHLERIPVVFKPNWDLYAKAYYTAKTLAMSDKMNPILFKAIQEDKNP
CCHHHHHHHCCCCCCEEECCEEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
LATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMSLMAHYQINAVPAFVVNNKYK
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCEEEEEECCCCC
TDLQMAGSEERLFEILNYLVRKSA
CHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKKLIALLFLMPMTALATQFIEGKDYQTVASAQLSTNKDKTPLITEFFSYGCPWCYKID
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEC
APLNDWATRMGKGAHLERIPVVFKPNWDLYAKAYYTAKTLAMSDKMNPILFKAIQEDKNP
CCHHHHHHHCCCCCCEEECCEEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
LATKQSMVDFFVAHGVDREIAKSAFENSPTIDMRVNSGMSLMAHYQINAVPAFVVNNKYK
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCEEEEEECCCCC
TDLQMAGSEERLFEILNYLVRKSA
CHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA