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The map label for this gene is proP [H]

Identifier: 148284480

GI number: 148284480

Start: 826664

End: 827884

Strand: Direct

Name: proP [H]

Synonym: OTBS_0844

Alternate gene names: 148284480

Gene position: 826664-827884 (Clockwise)

Preceding gene: 148284479

Following gene: 148284481

Centisome position: 38.86

GC content: 29.65

Gene sequence:

>1221_bases
ATGAGTAACAAAGATCTGATAGTTGCATATTTTGCTAGTATCGTCCAAGTATGTAATTATGCATTATTTGGGCATGCAAT
TACTTTTTTAGCTGCTGAATATATGCCACCTGATGTTTCAACTAATCCTACTACCAGTATCTTTATTTTGCTAGGAGCCA
CTTCTATTGCTAAACCAATAGGAGCGGTAATATATGGGATTATAGGTGATTATTATAGCCGAAGATTAGCAATTAGAACA
GCTTCATTACTATCAACACTAGCTGTAATCTCTATTGGGTTTATACCTGCATACCAAAGAATAGGGATGTTATCGAGCAT
TTTATTTATTCTATGCCGTAGTATTTTTATTATGGGGTTAATAGGTGAGACTGATGGAGTGCGAATATATGTATTTGAAG
CAGTAGATAAGTACAAAAACTTAGCGAACGGAATAATAGCTGGTTGTTGGCAAATAGGAGCACAATTAGCTGTATTGCTT
TTATTAGTAGCAAATATTAATAATTTTCAATATAGTTGGCGCTTCTGTTTTATTACTGGAGGAGTATTAGGATTGTTGGT
GTTTTTTCAGAGTAAACATTTAAAGATTAATAATATCAAAACTTCAAAAACTACAACAGCAAGCAAATTATTTCAGTCCT
GTAAACTTATACCAGGATATTGGAGGTTACTAATACCCTGTATAATTATACAGGGATGTATTGGTGCTATTTATAGTTTT
CAACTATATTTTTTCCCTGTATATGTTATTAAAATGCTAAACATTGTTGACTTCAATAATATGAATTTTACTATTGTATT
AGCATTATTATTATATTCTATAATGGCTGTATGGTCAGGATATTTAGCAGATAAATATTACTTGCATTATCAAACCATCT
TTTCTGCTTTAATGTTAAGTATAGTTTTCAGTACAGTTAATATGGCTATGATGATAAATTATCAAGAATTCTTACCAGTA
TTATATATTATACCAATTCTATTAATACCTTTTTATTCTGTTCCGCTACAGGTAAAAGTATGTAAATTATTTTCTGATAA
TATTAGACTAAGATTGTTTAGCTTATCACATTCTATTGGATCAGTATTAATATCAGCACAAATATGTGCTATATCAGCAT
TAGCATATAATAAGGCTAATTTTTTGCCAATGCTGATTTTAATCTTATTATTTCTTATCTTAGGGCTTACAGTATTAGTA
ATAAAACCTAGAATACAGTAA

Upstream 100 bases:

>100_bases
GGTAGTTTTAATATTTATTAATTTGTAGGTAAAAGTCCTGATAAACTACAACATAATCTATAGTATTATTTTATTTTTAT
AAGAAGCTTTAGAAAATTTT

Downstream 100 bases:

>100_bases
ATTATGTCTTGTTAATACTGTTTTGACACATTATTGTTTTTAATGTTAACTTATATAGCTAAACTACTATAAAACAATTT
TAATAATAAGATGTTAAGAT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSNKDLIVAYFASIVQVCNYALFGHAITFLAAEYMPPDVSTNPTTSIFILLGATSIAKPIGAVIYGIIGDYYSRRLAIRT
ASLLSTLAVISIGFIPAYQRIGMLSSILFILCRSIFIMGLIGETDGVRIYVFEAVDKYKNLANGIIAGCWQIGAQLAVLL
LLVANINNFQYSWRFCFITGGVLGLLVFFQSKHLKINNIKTSKTTTASKLFQSCKLIPGYWRLLIPCIIIQGCIGAIYSF
QLYFFPVYVIKMLNIVDFNNMNFTIVLALLLYSIMAVWSGYLADKYYLHYQTIFSALMLSIVFSTVNMAMMINYQEFLPV
LYIIPILLIPFYSVPLQVKVCKLFSDNIRLRLFSLSHSIGSVLISAQICAISALAYNKANFLPMLILILLFLILGLTVLV
IKPRIQ

Sequences:

>Translated_406_residues
MSNKDLIVAYFASIVQVCNYALFGHAITFLAAEYMPPDVSTNPTTSIFILLGATSIAKPIGAVIYGIIGDYYSRRLAIRT
ASLLSTLAVISIGFIPAYQRIGMLSSILFILCRSIFIMGLIGETDGVRIYVFEAVDKYKNLANGIIAGCWQIGAQLAVLL
LLVANINNFQYSWRFCFITGGVLGLLVFFQSKHLKINNIKTSKTTTASKLFQSCKLIPGYWRLLIPCIIIQGCIGAIYSF
QLYFFPVYVIKMLNIVDFNNMNFTIVLALLLYSIMAVWSGYLADKYYLHYQTIFSALMLSIVFSTVNMAMMINYQEFLPV
LYIIPILLIPFYSVPLQVKVCKLFSDNIRLRLFSLSHSIGSVLISAQICAISALAYNKANFLPMLILILLFLILGLTVLV
IKPRIQ
>Mature_405_residues
SNKDLIVAYFASIVQVCNYALFGHAITFLAAEYMPPDVSTNPTTSIFILLGATSIAKPIGAVIYGIIGDYYSRRLAIRTA
SLLSTLAVISIGFIPAYQRIGMLSSILFILCRSIFIMGLIGETDGVRIYVFEAVDKYKNLANGIIAGCWQIGAQLAVLLL
LVANINNFQYSWRFCFITGGVLGLLVFFQSKHLKINNIKTSKTTTASKLFQSCKLIPGYWRLLIPCIIIQGCIGAIYSFQ
LYFFPVYVIKMLNIVDFNNMNFTIVLALLLYSIMAVWSGYLADKYYLHYQTIFSALMLSIVFSTVNMAMMINYQEFLPVL
YIIPILLIPFYSVPLQVKVCKLFSDNIRLRLFSLSHSIGSVLISAQICAISALAYNKANFLPMLILILLFLILGLTVLVI
KPRIQ

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=306, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45409; Mature: 45278

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKDLIVAYFASIVQVCNYALFGHAITFLAAEYMPPDVSTNPTTSIFILLGATSIAKPI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHH
GAVIYGIIGDYYSRRLAIRTASLLSTLAVISIGFIPAYQRIGMLSSILFILCRSIFIMGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGETDGVRIYVFEAVDKYKNLANGIIAGCWQIGAQLAVLLLLVANINNFQYSWRFCFITG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHH
GVLGLLVFFQSKHLKINNIKTSKTTTASKLFQSCKLIPGYWRLLIPCIIIQGCIGAIYSF
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLYFFPVYVIKMLNIVDFNNMNFTIVLALLLYSIMAVWSGYLADKYYLHYQTIFSALMLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVFSTVNMAMMINYQEFLPVLYIIPILLIPFYSVPLQVKVCKLFSDNIRLRLFSLSHSIG
HHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHH
SVLISAQICAISALAYNKANFLPMLILILLFLILGLTVLVIKPRIQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNKDLIVAYFASIVQVCNYALFGHAITFLAAEYMPPDVSTNPTTSIFILLGATSIAKPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHH
GAVIYGIIGDYYSRRLAIRTASLLSTLAVISIGFIPAYQRIGMLSSILFILCRSIFIMGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGETDGVRIYVFEAVDKYKNLANGIIAGCWQIGAQLAVLLLLVANINNFQYSWRFCFITG
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHH
GVLGLLVFFQSKHLKINNIKTSKTTTASKLFQSCKLIPGYWRLLIPCIIIQGCIGAIYSF
HHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QLYFFPVYVIKMLNIVDFNNMNFTIVLALLLYSIMAVWSGYLADKYYLHYQTIFSALMLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVFSTVNMAMMINYQEFLPVLYIIPILLIPFYSVPLQVKVCKLFSDNIRLRLFSLSHSIG
HHHHHHHHHHEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHH
SVLISAQICAISALAYNKANFLPMLILILLFLILGLTVLVIKPRIQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]