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The map label for this gene is hemC

Identifier: 148284479

GI number: 148284479

Start: 825467

End: 826363

Strand: Direct

Name: hemC

Synonym: OTBS_0843

Alternate gene names: 148284479

Gene position: 825467-826363 (Clockwise)

Preceding gene: 148284466

Following gene: 148284480

Centisome position: 38.81

GC content: 32.11

Gene sequence:

>897_bases
ATGATTATTAGAGTAGGTAGTAGAGCAAGCAGATTGGCATTGATACAGGCACAAGCAGTAGTTCAAAAAATTAATGATTT
ACTTGGGTTAAATGCTATAATAATTCCTATTAAAACTACTGGTGATTTAATTCAAAATAAAAACCTTTATGATATAGGAG
GTAAAGGGCTATTTTTAAAAGAAATAGAGTATGCCTTATTGAATAATACTATTGATATTGCTGTTCATTCCTTAAAAGAT
GTGCCAGCTTATTTACCTGATGGATTACAGCTTGCAGCTGTACTAGAAAGAGGTGATGTTGGCGATATGCTTGTATCTAA
AATAGCAAATAAAATTACTGACTTGCCTTTAGGTGCAATAGTTGGAACTTCTTCTGTACGAAGACGAATACAGCTATTAA
TGCTTCGTCCTGATCTTAATATTGTGTTATTTAGAGGTAATGTTGATACCAGATGGAATAAAATTATCAATAATGAGGTA
GATGCAACAGTTTTAGCTGCTGCTGGACTACAGAGATTGAATTATGATACTAGCCGTTTTTGTAATATTATTCCTCAAAG
TGAGATGTTGCCAGCAATTGGGCAAGGAGCAATTGCAATTGAAGCTAGAAAAGACGATAAACTAATAATGCCATTATGTG
CTAAAATTAATCATCAGTTAACTTGGCAATTAATACAAGTCGAAAGAGGTTATTTAAAAACTTTAAATGCAGATTGTAAC
GTTCCAATAGGTGGGATTGCAAGCTATATTGGTAATAATAGTTTTGAGGCTAAATTTATGCTTGGAGATTATAATATGAG
ATATTTTTTTTATTCTGAGGTGCGTGGAAAGTTACAACATGGTTACGATATTGGAGTTGAAGCAGCAAAAAATTTTCAAA
AAAGATTATTTATCTGA

Upstream 100 bases:

>100_bases
AAAAGCTATTTTTTTATACTAATTTATGAATTTATATAGTTTAAATATACACTTATTCAGTGTGGAATAATAAGTCAGTG
AAAGTAGTAATATCAAGTTT

Downstream 100 bases:

>100_bases
ATTTTATTTGATCTATTTAGCAACTATATGTCTATGTTATGCTATCCAACATCTAATAATTAGGGAAAGCTTTAGCTAAA
ATACGTATATGATGAAACGA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MIIRVGSRASRLALIQAQAVVQKINDLLGLNAIIIPIKTTGDLIQNKNLYDIGGKGLFLKEIEYALLNNTIDIAVHSLKD
VPAYLPDGLQLAAVLERGDVGDMLVSKIANKITDLPLGAIVGTSSVRRRIQLLMLRPDLNIVLFRGNVDTRWNKIINNEV
DATVLAAAGLQRLNYDTSRFCNIIPQSEMLPAIGQGAIAIEARKDDKLIMPLCAKINHQLTWQLIQVERGYLKTLNADCN
VPIGGIASYIGNNSFEAKFMLGDYNMRYFFYSEVRGKLQHGYDIGVEAAKNFQKRLFI

Sequences:

>Translated_298_residues
MIIRVGSRASRLALIQAQAVVQKINDLLGLNAIIIPIKTTGDLIQNKNLYDIGGKGLFLKEIEYALLNNTIDIAVHSLKD
VPAYLPDGLQLAAVLERGDVGDMLVSKIANKITDLPLGAIVGTSSVRRRIQLLMLRPDLNIVLFRGNVDTRWNKIINNEV
DATVLAAAGLQRLNYDTSRFCNIIPQSEMLPAIGQGAIAIEARKDDKLIMPLCAKINHQLTWQLIQVERGYLKTLNADCN
VPIGGIASYIGNNSFEAKFMLGDYNMRYFFYSEVRGKLQHGYDIGVEAAKNFQKRLFI
>Mature_298_residues
MIIRVGSRASRLALIQAQAVVQKINDLLGLNAIIIPIKTTGDLIQNKNLYDIGGKGLFLKEIEYALLNNTIDIAVHSLKD
VPAYLPDGLQLAAVLERGDVGDMLVSKIANKITDLPLGAIVGTSSVRRRIQLLMLRPDLNIVLFRGNVDTRWNKIINNEV
DATVLAAAGLQRLNYDTSRFCNIIPQSEMLPAIGQGAIAIEARKDDKLIMPLCAKINHQLTWQLIQVERGYLKTLNADCN
VPIGGIASYIGNNSFEAKFMLGDYNMRYFFYSEVRGKLQHGYDIGVEAAKNFQKRLFI

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=274, Percent_Identity=37.2262773722628, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI20149500, Length=274, Percent_Identity=37.2262773722628, Blast_Score=161, Evalue=6e-40,
Organism=Escherichia coli, GI48994974, Length=292, Percent_Identity=42.4657534246575, Blast_Score=208, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319996, Length=257, Percent_Identity=36.9649805447471, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI20130425, Length=248, Percent_Identity=39.1129032258064, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_ORITB (A5CDH4)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248569.1
- ProteinModelPortal:   A5CDH4
- SMR:   A5CDH4
- STRING:   A5CDH4
- GeneID:   5219430
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0843
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   DADILEM
- ProtClustDB:   PRK00072
- BioCyc:   OTSU357244:OTBS_0843-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33082; Mature: 33082

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIRVGSRASRLALIQAQAVVQKINDLLGLNAIIIPIKTTGDLIQNKNLYDIGGKGLFLK
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCEEEECCCEEEHH
EIEYALLNNTIDIAVHSLKDVPAYLPDGLQLAAVLERGDVGDMLVSKIANKITDLPLGAI
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHH
VGTSSVRRRIQLLMLRPDLNIVLFRGNVDTRWNKIINNEVDATVLAAAGLQRLNYDTSRF
CCCHHHHHHEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
CNIIPQSEMLPAIGQGAIAIEARKDDKLIMPLCAKINHQLTWQLIQVERGYLKTLNADCN
HHCCCCHHCCCCCCCCEEEEEECCCCCEEHHHHHHCCHHHEEEEEEECCCHHEECCCCCC
VPIGGIASYIGNNSFEAKFMLGDYNMRYFFYSEVRGKLQHGYDIGVEAAKNFQKRLFI
CCHHHHHHHHCCCCCEEEEEEECCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIIRVGSRASRLALIQAQAVVQKINDLLGLNAIIIPIKTTGDLIQNKNLYDIGGKGLFLK
CEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCEEEECCCEEEHH
EIEYALLNNTIDIAVHSLKDVPAYLPDGLQLAAVLERGDVGDMLVSKIANKITDLPLGAI
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHH
VGTSSVRRRIQLLMLRPDLNIVLFRGNVDTRWNKIINNEVDATVLAAAGLQRLNYDTSRF
CCCHHHHHHEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
CNIIPQSEMLPAIGQGAIAIEARKDDKLIMPLCAKINHQLTWQLIQVERGYLKTLNADCN
HHCCCCHHCCCCCCCCEEEEEECCCCCEEHHHHHHCCHHHEEEEEEECCCHHEECCCCCC
VPIGGIASYIGNNSFEAKFMLGDYNMRYFFYSEVRGKLQHGYDIGVEAAKNFQKRLFI
CCHHHHHHHHCCCCCEEEEEEECCCEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA