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The map label for this gene is hemC

Identifier: 148268151

GI number: 148268151

Start: 1826785

End: 1827711

Strand: Reverse

Name: hemC

Synonym: SaurJH9_1728

Alternate gene names: 148268151

Gene position: 1827711-1826785 (Counterclockwise)

Preceding gene: 148268152

Following gene: 148268150

Centisome position: 62.88

GC content: 34.41

Gene sequence:

>927_bases
ATGCGTAAATTAGTCGTTGGCTCCAGAAGAAGTAAATTAGCTTTAACACAAAGCCAGCAATTTATTAATAAATTAAAAGC
TGTCGAGCCAAATCTAGAAATTGAAATTAAAGAAATTGTCACGAAAGGCGATCGTATAGTAGATAAACAATTGTCTAAAG
TCGGAGGCAAAGGCTTATTTGTTAAAGAAATACAACATGAACTTTTTGAAAAAAATATCGATATGGCAATACACTCGCTT
AAAGACGTACCAAGTGTAATTCCGGAAGGTTTAACATTAGGTTGTATCCCTGATAGAGAATTACCTTTTGATGCGTATAT
TTCTAAAACACATACACCACTATCCCAATTGCCAGAAGGCAGTATTATTGGTACTAGTTCATTACGTCGTGGTGCACAAA
TATTATCTAAGTATCCTAATTTAGAGATTAAATGGATTAGAGGTAATATAGATACACGATTAGAAAAGTTACAAACTGAA
GATTATGATGCGATTATTTTAGCTGCAGCTGGTTTAAGAAGAATGGGCTGGTCAGATGATATTGTAACATCTTATCTTGA
TAGAGATACATTGTTACCTGCAATCGGACAAGGTGCTTTAGGGATAGAATGTCGTAGTGACGATGAAGAACTATTAACAT
TATTAAGCAAAGTACATAATGATGAGGTTGCAAAATGTGTGACTGCTGAACGAACGTTTTTAGCAGAAATGGATGGTAGT
TGTCAGGTGCCAATCGCAGGATATGCTACAATCTCAGATCAAAAAGAAATCGAATTTACAGGTTTAATTATGACCCCAGA
TGGTAAAGAACGATTTGAATATACAATGAACGGAACAGATCCGGTTGAGTTAGGCAAAACAGTGAGTAACAAATTAAAAG
AGCAAGGTGCTTATGAAATTATAAAACGCTTAAATGAACAACATTAA

Upstream 100 bases:

>100_bases
TTATTTTATTTTGCTTAAATATGATTAATTTATTTTTTGCAACACATTTTGTGAATTGAGAAAATAATCTAGAGCAATCC
GTTCCTAGGAGGAATTGAAC

Downstream 100 bases:

>100_bases
ATAATGGAGTGACGTTTAAATATGAAGCCAGTTGTAGTTATGACACAAACAAATGACATGCAAAGTGATTTAGTATCAAT
TATTCACAAACCCTTTATTG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSL
KDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGS
CQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH

Sequences:

>Translated_308_residues
MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSL
KDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGS
CQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH
>Mature_308_residues
MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSL
KDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGS
CQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=286, Percent_Identity=43.7062937062937, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI66933009, Length=286, Percent_Identity=43.7062937062937, Blast_Score=238, Evalue=4e-63,
Organism=Escherichia coli, GI48994974, Length=300, Percent_Identity=46, Blast_Score=249, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319996, Length=318, Percent_Identity=35.2201257861635, Blast_Score=191, Evalue=9e-50,
Organism=Drosophila melanogaster, GI20130425, Length=277, Percent_Identity=40.4332129963899, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_STAA1 (A7X391)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442247.1
- ProteinModelPortal:   A7X391
- SMR:   A7X391
- STRING:   A7X391
- EnsemblBacteria:   EBSTAT00000004754
- GeneID:   5560277
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1657
- eggNOG:   COG0181
- GeneTree:   EBGT00050000025230
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   SAUR418127:SAHV_1657-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34368; Mature: 34368

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLF
CCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCH
VKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCCHHHCCCC
SIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTEDYDAIILAAAGLRRMGWSDD
CEECHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEHHHHHHCCCCHH
IVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGS
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
CQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEI
CCCCCCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHH
IKRLNEQH
HHHHHCCC
>Mature Secondary Structure
MRKLVVGSRRSKLALTQSQQFINKLKAVEPNLEIEIKEIVTKGDRIVDKQLSKVGGKGLF
CCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHHHHHHHHHHCCCCCH
VKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELPFDAYISKTHTPLSQLPEG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCCHHHCCCC
SIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTEDYDAIILAAAGLRRMGWSDD
CEECHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEHHHHHHCCCCHH
IVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKVHNDEVAKCVTAERTFLAEMDGS
HHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
CQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTMNGTDPVELGKTVSNKLKEQGAYEI
CCCCCCCEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHH
IKRLNEQH
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA