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The map label for this gene is nfo

Identifier: 148268041

GI number: 148268041

Start: 1715890

End: 1716780

Strand: Reverse

Name: nfo

Synonym: SaurJH9_1615

Alternate gene names: 148268041

Gene position: 1716780-1715890 (Counterclockwise)

Preceding gene: 148268042

Following gene: 148268040

Centisome position: 59.06

GC content: 33.33

Gene sequence:

>891_bases
ATGTTATTAGGATCACATGTTTCAATGAGTGGTAAAAAGATGTTAGAAGGTTCTGCTATAGAAGCGTATGAATATGGTGA
AACAACATTTATGATTTATACTGGTGCACCTCAAAACACGCGCCGTAAAAGTATTGAAGATTTAAATATAACTAAAGGTC
ATGAAGTGATGGAAAAATATGGCTTATCTAATATTGTTGTTCATGCACCATACATCATTAATATTGCAAATACAACCAAA
CCAGAAACGTTTAATCTTGGTGTAGATTTCTTGCAACAAGAAATTGAAAGAACGCAAGCTATAGGCGCTAAAGATATTGT
ATTACATCCAGGTGCGCATGTTGGAGCTGGTGTTGATGCCGGAATTAATAAGATTATTGAAGGATTAAATGAAGTTTTAA
CGAATGACAATAATGTACGTATTGCGCTTGAAACGATGGCAGGTAAAGGTACAGAGATTGGTCGCTCGTTTGAAGAGTTA
GCACGTATTATTGATGGAGTTCATAATAACGAACGTTTATCAGTATGTTTTGATACATGTCATACGCATGATGCTGGATA
TAATGTGAAAGAGGACTTTGATGGCGTATTAAATGAATTTGATAAAATTATTGGAGTCGACAGAATCAAAGTTGTTCATG
TCAATGATTCTAAAAATGACCGTGGCGCTCAAAAAGACCGACATGAAAATATTGGCTTCGGTTACATTGGTTTTGATGCA
TTAAATTACATTGTGCATCATGATAGTTTTAAAGATATTCCGAAAATCTTAGAAACACCTTATGTTGGTGAAGATAAGAA
AAATAAAAAACCGCCATATAAACTTGAAATTGAAATGTTAAAACAACAGCAATTTGACCCAGAATTAAAAAATAAGGTTA
TGCAACAATAA

Upstream 100 bases:

>100_bases
ACAAGAAGTTGAAAAAATGAAACGTCAAGAGCGTAAACAATTTAGTAAGCAGCAAAATAGACAAAAACGTAAGCAAAACA
AAAAAGGTTAGGTGTGTTTT

Downstream 100 bases:

>100_bases
AATGATAATGCTCTCTTCAAGAAATGCGATAAATAAAACTTGAAGGGGGCATATCTCTTTATTTTGTCTAATTTTGAGTC
GTAAACATTACTGTTTACAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ

Sequences:

>Translated_296_residues
MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
>Mature_296_residues
MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTK
PETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=289, Percent_Identity=32.1799307958478, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531193, Length=264, Percent_Identity=32.5757575757576, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322735, Length=264, Percent_Identity=31.4393939393939, Blast_Score=147, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_STAA1 (A7X2V2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442134.1
- ProteinModelPortal:   A7X2V2
- SMR:   A7X2V2
- STRING:   A7X2V2
- EnsemblBacteria:   EBSTAT00000004447
- GeneID:   5560107
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1544
- eggNOG:   COG0648
- GeneTree:   EBGT00050000024900
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   SAUR418127:SAHV_1544-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 33185; Mature: 33185

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKY
CCCCCCCCCCCHHHHCCCCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHH
GLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDA
CCCCEEEECCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
GINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHH
HTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
HCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHH
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLLGSHVSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKY
CCCCCCCCCCCHHHHCCCCEEEEECCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHH
GLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDA
CCCCEEEECCEEEEECCCCCCCEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
GINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTC
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHH
HTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDA
HCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHH
LNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLKQQQFDPELKNKVMQQ
HHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA