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The map label for this gene is mraW

Identifier: 148267671

GI number: 148267671

Start: 1281301

End: 1282236

Strand: Direct

Name: mraW

Synonym: SaurJH9_1238

Alternate gene names: 148267671

Gene position: 1281301-1282236 (Clockwise)

Preceding gene: 148267670

Following gene: 148267672

Centisome position: 44.08

GC content: 36.0

Gene sequence:

>936_bases
GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAAAATGGTGTGTACATTGACTG
TACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAATCAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAG
ACCAAACTGCAATTGATAATGCTAAAGAGGTATTAAAGGATCATTTGCATAAGGTGACTTTTGTTCATAGCAACTTCCGT
GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGACTTGGGTGTTTCAAGCCCACA
ACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGACGCAACATTAGACATGCGTATGGACCAAACACAAGAACTAA
CAGCATATGAAATTGTTAACAATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGTTATGGCGAGGAGAAATTTTCA
AAACAGATAGCTCGAAGAATCGAAGCACATCGTGAACAACAACCAATAACAACAACATTAGAATTAGTTGACATTATAAA
AGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACATCCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAA
ACGATGAATTGTCAGCTTTTGAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGACGGCAGGATTTCGGTAATC
ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGTCCAGAGGTACCAAGAGGATT
GCCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGTGTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAG
ATGACAATAACAGAGCACGAAGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA

Upstream 100 bases:

>100_bases
TAGAGAAACTTGGAATGATTTCTATGAAGAATCTGAAGAAAGTTTCGAAGATATTGCTGAAGATTTAATAGATTTTGATT
TTTAAAATGGAGGAATTGAA

Downstream 100 bases:

>100_bases
GGAGCAATTTATAATGGCTGTAGAAAAAGTGTACCAACCATATGACGAACAAGTTTATAATAGTATACCGAAGCAACAAC
CACAAACTAAGCCCGAAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

Sequences:

>Translated_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK
>Mature_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=33.1378299120235, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=36.8421052631579, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=41.0828025477707, Blast_Score=244, Evalue=5e-66,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=35.1681957186544, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=35.1681957186544, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_STAA1 (A7X1B8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441759.1
- ProteinModelPortal:   A7X1B8
- STRING:   A7X1B8
- EnsemblBacteria:   EBSTAT00000004356
- GeneID:   5559914
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1169
- eggNOG:   COG0275
- GeneTree:   EBGT00050000024888
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   SAUR418127:SAHV_1169-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35682; Mature: 35682

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC
>Mature Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA