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The map label for this gene is gyaR [H]

Identifier: 148267363

GI number: 148267363

Start: 975857

End: 976816

Strand: Direct

Name: gyaR [H]

Synonym: SaurJH9_0929

Alternate gene names: 148267363

Gene position: 975857-976816 (Clockwise)

Preceding gene: 148267362

Following gene: 148267364

Centisome position: 33.57

GC content: 34.27

Gene sequence:

>960_bases
TTGGTAAAAATAGTTGTTTCGAGGAAAATTCCAGATAAATTTTATCAACAATTAAGTAAACTTGGTGACGTTGTTATGTG
GCAAAAATCATTAGTGCCTATGCCTAAAGATCAATTTGTGACAGCACTTCGTGACGCAGATGCTTGTTTTATTACATTAA
GTGAACAGATCGATGCAGAAATTTTAGCACAATCACCAAATTTAAAAGTAATTGCGAATATGGCTGTAGGATATGACAAC
ATCGATGTTGAAAGTGCAACAGCGAATAACGTGGTTGTCACGAATACACCAAATGTACTTACTGAAACAACTGCAGAATT
AGGATTTACATTAATGCTTGCTATAGCACGCCGTATTGTAGAAGCTGAAAAATATGTAGAAGCAGATGCATGGCAAAGCT
GGGGTCCTTATTTATTGTCAGGTAAAGATGTCTTCAATTCAACTATTGGAATATATGGTATGGGAGATATTGGTAAAGCT
TTTGCAAGAAGGTTGCAAGGGTTTAATACTAATATTCTTTATCATAATCGATCAAGACATAAAGATGCAGAGGCGGACTT
TAATGCAACATATGTTTCTTTTGAAACGTTGTTAGCAGAAAGTGATTTTATCATCTGTACAGCGCCACTTACAAAAGAAA
CACATCATAAATTTAATGCTGAAGCATTTGAACAAATGAAAAATGATGCAATTTTTATTAATATCGGTAGAGGGCAAATT
GTAGATGAAACAGCATTAATCGATGCACTAGACAATAAAGAAATTTTAGCATGTGGTTTAGATGTATTAGCAAATGAACC
GATTGATCATACACATCCATTAATGGGACGTGATAATGTTCTGATTACACCACACATTGGTAGCGCATCAGTAACAACAC
GGGACAATATGATTCAATTATGTATTAATAATATAGAAGCGGTTATGACAAATCAGGTACCACATACTCCAGTAAATTGA

Upstream 100 bases:

>100_bases
ATAAAAATGTGCCACCAACGTATTCTTTTAAAGATTTAAATGAAGCAATAGCTGAATTAGAAAAATAGATATAGTCATTT
TATAAAGTAGGTGAATTGAT

Downstream 100 bases:

>100_bases
AAATAAGTATGGTTTTTAATCATTGGTAAAACAAGCAAAGCAATTGTTAAATATGTTATGCTTGACTTGCGAATGTATAT
ATTTGCGCTGATGATAATTC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAEILAQSPNLKVIANMAVGYDN
IDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQI
VDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN

Sequences:

>Translated_319_residues
MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAEILAQSPNLKVIANMAVGYDN
IDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQI
VDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN
>Mature_319_residues
MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAEILAQSPNLKVIANMAVGYDN
IDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQI
VDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=39.0476190476191, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI23308577, Length=257, Percent_Identity=28.4046692607004, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI61743967, Length=261, Percent_Identity=29.8850574712644, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=30.0395256916996, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI145580578, Length=259, Percent_Identity=27.027027027027, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4557499, Length=259, Percent_Identity=27.027027027027, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI145580575, Length=271, Percent_Identity=27.6752767527675, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI87082289, Length=318, Percent_Identity=42.4528301886792, Blast_Score=240, Evalue=7e-65,
Organism=Escherichia coli, GI1787645, Length=200, Percent_Identity=34.5, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789279, Length=271, Percent_Identity=27.3062730627306, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081824, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788660, Length=272, Percent_Identity=25.7352941176471, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=279, Percent_Identity=31.1827956989247, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=255, Percent_Identity=28.6274509803922, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=314, Percent_Identity=31.5286624203822, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320925, Length=270, Percent_Identity=27.037037037037, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=282, Percent_Identity=27.6595744680851, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6325144, Length=174, Percent_Identity=32.183908045977, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=307, Percent_Identity=24.1042345276873, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=33.7209302325581, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=40.7051282051282, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI45551003, Length=305, Percent_Identity=38.3606557377049, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI28574284, Length=305, Percent_Identity=38.3606557377049, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI45552429, Length=305, Percent_Identity=38.3606557377049, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24585514, Length=305, Percent_Identity=38.3606557377049, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI28574282, Length=305, Percent_Identity=38.3606557377049, Blast_Score=217, Evalue=9e-57,
Organism=Drosophila melanogaster, GI28574286, Length=276, Percent_Identity=40.2173913043478, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24585516, Length=276, Percent_Identity=36.5942028985507, Blast_Score=188, Evalue=3e-48,
Organism=Drosophila melanogaster, GI19921140, Length=291, Percent_Identity=27.4914089347079, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24646446, Length=266, Percent_Identity=27.0676691729323, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24646448, Length=266, Percent_Identity=27.0676691729323, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24646452, Length=266, Percent_Identity=27.0676691729323, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24646450, Length=266, Percent_Identity=27.0676691729323, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI62472511, Length=256, Percent_Identity=26.5625, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAE
CEEEEEECCCCHHHHHHHHHHHHEEEHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
ILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIV
HEECCCCEEEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKAFARRLQGFNTNILYHNRSRH
HHHHHHHHHCCCCCCCEEEECHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEEECCCCC
KDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQI
CCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHHCCCEEEEECCCCCE
VDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQL
ECHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHH
CINNIEAVMTNQVPHTPVN
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAE
CEEEEEECCCCHHHHHHHHHHHHEEEHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
ILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIV
HEECCCCEEEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKAFARRLQGFNTNILYHNRSRH
HHHHHHHHHCCCCCCCEEEECHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEEECCCCC
KDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQI
CCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHHCCCEEEEECCCCCE
VDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQL
ECHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHH
CINNIEAVMTNQVPHTPVN
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA