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The map label for this gene is gcvH

Identifier: 148267267

GI number: 148267267

Start: 907299

End: 907679

Strand: Direct

Name: gcvH

Synonym: SaurJH9_0832

Alternate gene names: 148267267

Gene position: 907299-907679 (Clockwise)

Preceding gene: 148267266

Following gene: 148267268

Centisome position: 31.21

GC content: 33.86

Gene sequence:

>381_bases
TTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAACAATTGGAAT
CACAGAATACGCACAAAGCGAGTTAGGTGATATTGTTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGG
ATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAAC
GAAGAACTAGAAGATAGTCCCGAATTTGTAAATGAATCTCCATACGAAAAAGCATGGATGGTAAAAGTAGAAATTAGTGA
TGAAAGTCAGCTTGAAGCTTTATTAACAGCTGAAAAATATTCAGAAATGATTGGTGAATAA

Upstream 100 bases:

>100_bases
AAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATGGCATCATTAATGATGTAAAGA
TTAATTAGGAGGGGATTCTC

Downstream 100 bases:

>100_bases
TCACCGTGTAACTCCTTAATCTAAGATTGAGGAGTTTATTTTTAGTCTGAGTAAGTAAATAATATGAAAGAAAATTTATT
AGGCACTATTATTTGGAGTA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVN
EELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE

Sequences:

>Translated_126_residues
MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVN
EELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE
>Mature_125_residues
AVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNE
ELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=45.2991452991453, Blast_Score=124, Evalue=2e-29,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=44.4444444444444, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=52.8455284552846, Blast_Score=135, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=50.8474576271186, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=48.695652173913, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=41.6, Blast_Score=109, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=47.1544715447154, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_STAA1 (A7X019)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441419.1
- ProteinModelPortal:   A7X019
- SMR:   A7X019
- STRING:   A7X019
- EnsemblBacteria:   EBSTAT00000003281
- GeneID:   5559114
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0829
- eggNOG:   COG0509
- GeneTree:   EBGT00050000023672
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   PRK01202
- BioCyc:   SAUR418127:SAHV_0829-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14081; Mature: 13949

Theoretical pI: Translated: 3.70; Mature: 3.70

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVE
CCCCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
SEMIGE
HHHHCC
>Mature Secondary Structure 
AVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVE
CCCCCCCCCCCCCEEEEECCEEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
SEMIGE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA