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The map label for this gene is nuc [H]

Identifier: 148267251

GI number: 148267251

Start: 897320

End: 898006

Strand: Direct

Name: nuc [H]

Synonym: SaurJH9_0816

Alternate gene names: 148267251

Gene position: 897320-898006 (Clockwise)

Preceding gene: 148267250

Following gene: 148267252

Centisome position: 30.87

GC content: 34.21

Gene sequence:

>687_bases
ATGACAGAATACTTATTAAGTGCTGGCATATGTATGGCAATTGTTTCAATATTACTTATAGGGATGGCTATCAGTAATGT
TTCGAAAGGGCAATACGCAAAGAGGTTTTTCTTTTTCGCTACTAGTTGCTTAGTGTTAACTTTAGTTGTAGTTTCAAGTC
TAAGTAGCTCAGCAAATGCATCACAAACAGATAACGGCGTAAATAGAAGTGGTTCTGAAGATCCAACAGTATATAGTGCA
ACTTCAACTAAAAAATTACATAAAGAACCTGCGACATTAATTAAAGCGATTGATGGTGATACGGTTAAATTAATGTACAA
AGGTCAACCAATGACATTCAGACTATTATTAGTTGATACACCTGAAACAAAGCATCCTAAAAAAGGTGTAGAGAAATATG
GCCCTGAAGCAAGTGCATTTACGAAAAAAATGGTAGAAAATGCAAATAAAATTGAAGTCGAGTTTGACAAAGGTCAAAGA
ACTGATAAATATGGACGTGGCTTAGCGTATATTTATGCTGATGGAAAAATGGTAAACGAAGCTTTAGTTCGTCAAGGCTT
GGCTAAAGTTGCTTATGTTTATAAACCTAACAATACACATGAACAACTTTTAAGAAAAAGTGAAGCACAAGCGAAAAAAG
AGAAATTAAATATTTGGAGCGAAGACAACGCTGATTCAGGTCAATAA

Upstream 100 bases:

>100_bases
TTATAATTATTAAATTTTTATTAATTAATTGTAAAAATGTAGAATTATAATTAATTAACGTTTAATATTAAAATTAACTA
AAAAGAAAGAGGTGTTAGTT

Downstream 100 bases:

>100_bases
TGCTCATTGTAAAAGTGTCACTGCTGCTAGTGGCCCTTTTATAATTTTTAGATCACGATATGATTTATTATCAATTCAGA
ATTAAAAAAGTAAATAGTAT

Product: micrococcal nuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSA
TSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQR
TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ

Sequences:

>Translated_228_residues
MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSA
TSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQR
TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ
>Mature_227_residues
TEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSAT
STKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQRT
DKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Nuclease B:Membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 25090; Mature: 24959

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS01123 TNASE_1 ; PS01284 TNASE_2 ; PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANA
CCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDT
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEC
PETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQRTDKYGRGLAYIYADGKMVNE
CCCCCCHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCEEEEEECCHHHHH
ALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANA
CHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDT
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEC
PETKHPKKGVEKYGPEASAFTKKMVENANKIEVEFDKGQRTDKYGRGLAYIYADGKMVNE
CCCCCCHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCEEEEEECCHHHHH
ALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNIWSEDNADSGQ
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6307819; 3921523; 3112123; 893427; 4324890; 4339720; 288045; 1896431; 1731948; 8069625 [H]