Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 148267234

GI number: 148267234

Start: 876970

End: 877731

Strand: Direct

Name: tpiA

Synonym: SaurJH9_0799

Alternate gene names: 148267234

Gene position: 876970-877731 (Clockwise)

Preceding gene: 148267233

Following gene: 148267235

Centisome position: 30.17

GC content: 36.88

Gene sequence:

>762_bases
ATGAGAACACCAATTATAGCTGGTAACTGGAAAATGAACAAAACAGTACAAGAAGCAAAAGACTTCGTCAATGCATTACC
AACATTACCAGATTCAAAAGAAGTAGAATCAGTAATTTGTGCACCAGCAATTCAATTAGATGCATTAACTACTGCAGTTA
AAGAAGGAAAAGCACAAGGTTTAGAAATCGGTGCTCAAAATACGTATTTCGAAGATAATGGTGCGTTCACAGGTGAAACG
TCTCCAGTTGCATTAGCAGATTTAGGCGTTAAATACGTTGTTATCGGTCATTCTGAACGTCGTGAATTATTCCACGAAAC
AGATGAAGAAATTAACAAAAAAGCGCACGCTATTTTCAAACATGGAATGACTCCAATTATTTGTGTTGGTGAAACAGACG
AAGAGCGTGAAAGTGGTAAAGCTAACGATGTTGTAGGTGAGCAAGTTAAGAAAGCTGTTGCAGGTTTATCTGAAGATCAA
CTTAAATCAGTTGTAATTGCTTATGAGCCAATCTGGGCAATCGGAACTGGTAAATCATCAACATCTGAAGATGCAAATGA
AATGTGTGCATTTGTACGTCAAACTATTGCTGACTTATCAAGCAAAGAAGTATCAGAAGCAACTCGTATTCAATATGGTG
GTAGTGTTAAACCTAACAACATTAAAGAATACATGGCACAAACTGATATTGATGGGGCATTAGTAGGTGGCGCATCACTT
AAAGTTGAAGATTTCGTACAATTGTTAGAAGGTGCAAAATAA

Upstream 100 bases:

>100_bases
TGTGGCATGTTTGTTTAACATTGTTACGGGAAAACAGTCACAAGATGACATCGTGTTTCATCACTTTTCAAAAATATTTA
CAAAACAAGGAGTGTCTTTA

Downstream 100 bases:

>100_bases
TCATGGCTAAGAAACCAACTGCGTTAATTATTTTAGATGGTTTTGCGAACCGCGAAAGCGAACATGGTAATGCGGTAAAA
TTAGCAAACAAGCCTAATTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGET
SPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASL
KVEDFVQLLEGAK

Sequences:

>Translated_253_residues
MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGET
SPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASL
KVEDFVQLLEGAK
>Mature_253_residues
MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGET
SPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASL
KVEDFVQLLEGAK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=42.3387096774194, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=42.3387096774194, Blast_Score=173, Evalue=2e-43,
Organism=Escherichia coli, GI1790353, Length=256, Percent_Identity=42.578125, Blast_Score=198, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=41.7670682730924, Blast_Score=183, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=41.9354838709677, Blast_Score=188, Evalue=9e-49,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=44.758064516129, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=44.758064516129, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=44.758064516129, Blast_Score=195, Evalue=3e-50,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_STAA1 (A7WZS8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441361.1
- ProteinModelPortal:   A7WZS8
- SMR:   A7WZS8
- STRING:   A7WZS8
- EnsemblBacteria:   EBSTAT00000004244
- GeneID:   5560549
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0771
- eggNOG:   COG0149
- GeneTree:   EBGT00050000024689
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   SAUR418127:SAHV_0771-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 97-97 ACT_SITE 169-169 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQG
CCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCC
LEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFK
EEECCCCCEECCCCCCCCCCCCEEHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
HGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQLKSVVIAYEPIWAIGTGKSS
CCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHEEEEECCEEEEECCCCC
TSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEECCCCC
KVEDFVQLLEGAK
CHHHHHHHHHCCC
>Mature Secondary Structure
MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAIQLDALTTAVKEGKAQG
CCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCC
LEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRELFHETDEEINKKAHAIFK
EEECCCCCEECCCCCCCCCCCCEEHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
HGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQLKSVVIAYEPIWAIGTGKSS
CCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHEEEEECCEEEEECCCCC
TSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKPNNIKEYMAQTDIDGALVGGASL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEEECCCCC
KVEDFVQLLEGAK
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA