| Definition | Staphylococcus aureus subsp. aureus JH9, complete genome. |
|---|---|
| Accession | NC_009487 |
| Length | 2,906,700 |
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The map label for this gene is dkgA [H]
Identifier: 148267163
GI number: 148267163
Start: 795305
End: 796144
Strand: Direct
Name: dkgA [H]
Synonym: SaurJH9_0727
Alternate gene names: 148267163
Gene position: 795305-796144 (Clockwise)
Preceding gene: 148267162
Following gene: 148267164
Centisome position: 27.36
GC content: 31.79
Gene sequence:
>840_bases ATGTTGAATGAGATACAAATATTAAATAATGGATACCCGATGCCTTCAGTTGGGTTAGGTGTTTATAAAATCTCTGACGA AGATATGACTAAAGTTGTAAATGCTGCAATTGACGCAGGCTATAGAGCGTTTGATACAGCATACTTTTATGATAATGAGG CTTCACTAGGACGAGCATTAAAGGATAATGGCGTCGATAGAGAAGATTTGTTTATAACAACGAAGTTATGGAATGACTAT CAAGGTTATGAGAAAACATTCGAATATTTCAACAAATCGATTGAAAATTTACAAACTGATTATCTTGATTTATTTCTAAT ACATTGGCCTTGTGAAGCAGATGGTCTATTTTTAGAAACATATAAAGCTATGGAAGAACTTTACGAGCAAGGTAAGGTAA AAGCAATAGGTGTATGTAATTTTAATGTTCATCATCTAGAAAAATTAATGGCTCAATCAAGTATCAAACCAATGGTGAAT CAAATTGAGGTACATCCATATTTTAACCAACAAGAATTACAAGAATTTTGTGATCGTCACGATATTAAAGTGACTGCATG GATGCCTTTGATGAGAAATAGAGGACTACTAGACAACCCTGTCATTGTTAAAATTGCTGAAAAATATCATAAAACACCAG CACAAGTTGTATTACGTTGGCATTTAGCACACAATAGAATTATTATTCCAAAATCTCAGACACCTAAACGCATTCAAGAA AATATAGATATTTTAGATTTTAATTTAGAATTAACAGAAGTAGCTGAAATTGATGCTTTAGATAGAAATGCAAGACAAGG TAAAAATCCAGATGATGTGAAAATTGGGGATTTAAAATAA
Upstream 100 bases:
>100_bases TATTTTTTATTGAAAGCGATAATTCGCATTAATTGAGTTTGTTGAAAAATTTAGGGTAATGTAAAGATATAAAAGATACA TAGATTGGAGAGATATAAAG
Downstream 100 bases:
>100_bases CTGGATGTTAAATTTTACGTTTATGAATGCCTTTTAATGTGTACATTAAAATAAATGAGTTGGTTTTTACTATTTGATAA AACAATACTCAGGTACATTC
Product: 2,5-didehydrogluconate reductase
Products: NA
Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]
Number of amino acids: Translated: 279; Mature: 279
Protein sequence:
>279_residues MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE NIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIGDLK
Sequences:
>Translated_279_residues MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE NIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIGDLK >Mature_279_residues MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAFDTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDY QGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE NIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIGDLK
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=39.9339933993399, Blast_Score=209, Evalue=2e-54, Organism=Homo sapiens, GI223468663, Length=291, Percent_Identity=39.8625429553265, Blast_Score=208, Evalue=5e-54, Organism=Homo sapiens, GI24497577, Length=297, Percent_Identity=37.3737373737374, Blast_Score=207, Evalue=1e-53, Organism=Homo sapiens, GI5174391, Length=297, Percent_Identity=37.3737373737374, Blast_Score=207, Evalue=1e-53, Organism=Homo sapiens, GI310109922, Length=277, Percent_Identity=37.5451263537906, Blast_Score=195, Evalue=3e-50, Organism=Homo sapiens, GI291291012, Length=286, Percent_Identity=38.4615384615385, Blast_Score=194, Evalue=1e-49, Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=35.9322033898305, Blast_Score=191, Evalue=8e-49, Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=35.5932203389831, Blast_Score=189, Evalue=2e-48, Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=35.5932203389831, Blast_Score=187, Evalue=7e-48, Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=35.5932203389831, Blast_Score=187, Evalue=7e-48, Organism=Homo sapiens, GI4502049, Length=292, Percent_Identity=39.3835616438356, Blast_Score=183, Evalue=2e-46, Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=34.4709897610922, Blast_Score=180, Evalue=1e-45, Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=33.8983050847458, Blast_Score=176, Evalue=2e-44, Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.1351351351351, Blast_Score=175, Evalue=3e-44, Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.1818181818182, Blast_Score=157, Evalue=7e-39, Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=37.012987012987, Blast_Score=112, Evalue=3e-25, Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=40.1785714285714, Blast_Score=91, Evalue=2e-18, Organism=Homo sapiens, GI207028673, Length=112, Percent_Identity=39.2857142857143, Blast_Score=88, Evalue=1e-17, Organism=Escherichia coli, GI87082198, Length=265, Percent_Identity=45.6603773584906, Blast_Score=263, Evalue=8e-72, Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=36.6141732283465, Blast_Score=183, Evalue=1e-47, Organism=Escherichia coli, GI1788081, Length=262, Percent_Identity=31.6793893129771, Blast_Score=115, Evalue=3e-27, Organism=Escherichia coli, GI87081735, Length=294, Percent_Identity=22.4489795918367, Blast_Score=71, Evalue=7e-14, Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=37.9310344827586, Blast_Score=198, Evalue=2e-51, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=38.2758620689655, Blast_Score=197, Evalue=3e-51, Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=37.7289377289377, Blast_Score=197, Evalue=6e-51, Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=36.0902255639098, Blast_Score=185, Evalue=2e-47, Organism=Caenorhabditis elegans, GI17564128, Length=295, Percent_Identity=34.9152542372881, Blast_Score=179, Evalue=1e-45, Organism=Caenorhabditis elegans, GI17537079, Length=296, Percent_Identity=32.4324324324324, Blast_Score=174, Evalue=6e-44, Organism=Caenorhabditis elegans, GI17561300, Length=269, Percent_Identity=36.8029739776952, Blast_Score=168, Evalue=3e-42, Organism=Caenorhabditis elegans, GI17566692, Length=294, Percent_Identity=34.6938775510204, Blast_Score=168, Evalue=3e-42, Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=34.6715328467153, Blast_Score=167, Evalue=5e-42, Organism=Caenorhabditis elegans, GI17538386, Length=270, Percent_Identity=34.8148148148148, Blast_Score=167, Evalue=8e-42, Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=32.319391634981, Blast_Score=163, Evalue=1e-40, Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=32.6460481099656, Blast_Score=160, Evalue=8e-40, Organism=Caenorhabditis elegans, GI17550246, Length=75, Percent_Identity=46.6666666666667, Blast_Score=66, Evalue=2e-11, Organism=Saccharomyces cerevisiae, GI6324694, Length=277, Percent_Identity=36.8231046931408, Blast_Score=185, Evalue=7e-48, Organism=Saccharomyces cerevisiae, GI6319625, Length=273, Percent_Identity=37.7289377289377, Blast_Score=179, Evalue=4e-46, Organism=Saccharomyces cerevisiae, GI6322556, Length=270, Percent_Identity=35.1851851851852, Blast_Score=179, Evalue=5e-46, Organism=Saccharomyces cerevisiae, GI6320576, Length=269, Percent_Identity=36.0594795539033, Blast_Score=174, Evalue=2e-44, Organism=Saccharomyces cerevisiae, GI6321896, Length=311, Percent_Identity=33.4405144694534, Blast_Score=164, Evalue=1e-41, Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=32.3741007194245, Blast_Score=132, Evalue=5e-32, Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=40.625, Blast_Score=213, Evalue=2e-55, Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=39.3728222996516, Blast_Score=203, Evalue=1e-52, Organism=Drosophila melanogaster, GI24644950, Length=297, Percent_Identity=39.0572390572391, Blast_Score=196, Evalue=2e-50, Organism=Drosophila melanogaster, GI21356425, Length=287, Percent_Identity=38.6759581881533, Blast_Score=193, Evalue=9e-50, Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=38.2252559726962, Blast_Score=189, Evalue=2e-48, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=36.5930599369085, Blast_Score=188, Evalue=4e-48, Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=34.2560553633218, Blast_Score=175, Evalue=3e-44, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=38.7543252595156, Blast_Score=174, Evalue=5e-44, Organism=Drosophila melanogaster, GI20129731, Length=283, Percent_Identity=33.9222614840989, Blast_Score=173, Evalue=1e-43, Organism=Drosophila melanogaster, GI24662785, Length=287, Percent_Identity=38.3275261324042, Blast_Score=171, Evalue=7e-43, Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=33.4630350194552, Blast_Score=149, Evalue=2e-36,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 32360; Mature: 32360
Theoretical pI: Translated: 4.97; Mature: 4.97
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 3.9 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAFDTAYFYDNEASLGRAL CCCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHH KDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLET HHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHH YKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVNQIEVHPYFNQQELQEFCDRH HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHC DIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE CCEEEEEHHHHHCCCCCCCCCEEEEHHHHCCCHHHHHHHEEECCCEEEECCCCCHHHHHC NIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIGDLK CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCCCEECCCC >Mature Secondary Structure MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAFDTAYFYDNEASLGRAL CCCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHH KDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYLDLFLIHWPCEADGLFLET HHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHH YKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVNQIEVHPYFNQQELQEFCDRH HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHC DIKVTAWMPLMRNRGLLDNPVIVKIAEKYHKTPAQVVLRWHLAHNRIIIPKSQTPKRIQE CCEEEEEHHHHHCCCCCCCCCEEEEHHHHCCCHHHHHHHEEECCCEEEECCCCCHHHHHC NIDILDFNLELTEVAEIDALDRNARQGKNPDDVKIGDLK CCCEEEECCCHHHHHHHHHHCCHHHCCCCCCCCEECCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]