Click here to switch to the map view.

The map label for this gene is mnhD2

Identifier: 148267084

GI number: 148267084

Start: 713572

End: 715068

Strand: Direct

Name: mnhD2

Synonym: SaurJH9_0648

Alternate gene names: 148267084

Gene position: 713572-715068 (Clockwise)

Preceding gene: 148267083

Following gene: 148267085

Centisome position: 24.55

GC content: 33.47

Gene sequence:

>1497_bases
ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTTGTATTTTTAAAAAATAATGA
TCGTATTTCTAAATATTTATACTTAGGTACAATGACTATCACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGC
GTCACCGTCCAATTACGCTAGACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTG
ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGTGGCGAACATAAAGCAAATCG
TTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGCGTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTAT
ACGTCATGTTTGAAATTATGTTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTACTTTATAAAACAGTAGGTAC
ATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATGGGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCT
TAGTCGCATTTAGTGCGAAAGCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTCACTTTACTATTTGATCAACA
TAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCTATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATA
AAGATATTAAAAAGATTGCAGCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTATTCTTTATTATTGGTAGTTT
AGTTTACATTACAGGCTATCGACAATATCAATATTTGAATGGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTA
TTATAATGATTTTTGCTATTGGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTACAGTTTATTTAGGATACTTTT
TTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAATTTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTA
TTTTAGTAGTTGTGGTTATCGCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA

Upstream 100 bases:

>100_bases
GTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTATAGAACTTATAAAGTAACAAAAGAAGATGAAATTGA
AGGCCTAAGGGGGGAAGATG

Downstream 100 bases:

>100_bases
ATGAATCAAATAGTTTTAAATATTATCATTGCATTCTTATGGGTATTATTTCAAGATGAAGATCATTTTAAATTCTCGAC
TTTCTTTTCCGGATATCTAA

Product: monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: Mrp complex subunit D2; Putative NADH-ubiquinone oxidoreductase subunit mnhD2

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED

Sequences:

>Translated_498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED
>Mature_498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED

Specific function: Expression of the mnh2 operon in E.coli is not able to catalyze Na(+)Li(+)/H(+) antiport. It does however confer higher growth rates than the control strain at up to pH 9.5. The operon may encode an NADH-ubiquinone oxidoreductase

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family

Homologues:

Organism=Homo sapiens, GI251831117, Length=361, Percent_Identity=25.7617728531856, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI251831116, Length=347, Percent_Identity=23.342939481268, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788829, Length=477, Percent_Identity=28.7211740041929, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788827, Length=383, Percent_Identity=24.2819843342037, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI2367154, Length=393, Percent_Identity=24.6819338422392, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788614, Length=502, Percent_Identity=24.7011952191235, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788613, Length=467, Percent_Identity=24.6252676659529, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788831, Length=388, Percent_Identity=23.1958762886598, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNHD2_STAAU (Q0Q2J7)

Other databases:

- EMBL:   DQ659239
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55154; Mature: 55154

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd1279a4)-; HASH(0xe0b3fa8)-; HASH(0xdfc0c78)-; HASH(0xe524ca8)-; HASH(0xe527d88)-; HASH(0xe5a667c)-; HASH(0xe52a6e0)-; HASH(0xe521bd0)-; HASH(0xe52a920)-; HASH(0xe51e96c)-; HASH(0xe524b58)-; HASH(0xe52a7e8)-; HASH(0xe51e87c)-; HASH(0xe51e9a8)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHCCCCCCCCH
>Mature Secondary Structure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CHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA