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The map label for this gene is cmtB [H]

Identifier: 148266819

GI number: 148266819

Start: 414139

End: 414582

Strand: Reverse

Name: cmtB [H]

Synonym: SaurJH9_0380

Alternate gene names: 148266819

Gene position: 414582-414139 (Counterclockwise)

Preceding gene: 148266820

Following gene: 148266818

Centisome position: 14.26

GC content: 35.36

Gene sequence:

>444_bases
GTGTCATTAGACATTTTGTCAACAACACGCATCATTGTAAAAGAACAAGTAAATGATTGGACTGAAGCTATAACTATAGC
TTCTCAGCCATTACTACAAGAACAAATTATTGAACAAGGCTATGTTCAAGCAATGATTGATAGCGTTAATGAACTTGGAC
CTTATATCGTTATCGCACCTGAAATTGCAATTGCACATGCAAGACCGAACAATGACGTACATCAAGTTGGTTTAAGTCTA
TTAAAGTTGAATCAACATGTGGCATTTTGTGATGAAGATCACTACGCATCTCTCATTTTTGTATTGAGTGCCATCGACAA
TCATTCACACTTATCTGTATTACAAAATTTAGCAACCGTACTGGGCGATAACCAAACAGTCCAGCAACTATTAACTGCAA
AAAACGCACAAGACATTAAAAACATTTTAAAGGAGCATGATTAA

Upstream 100 bases:

>100_bases
TGCTTAAGCAACGACTTATATCGACAAGATTTCTTAGATGGGAATATTTTTAAAATTAAACAACACATTGCTTTAACTAT
GACAAAGGAGGTTTAATAAC

Downstream 100 bases:

>100_bases
TATGAAAATTTTAGTAGTATGTGGCCACGGTTTAGGAAGTAGTTTTATGGTAGAAATGAACGCACAAGAAGCACTTAGGC
AACTTAATGCACCATCTGAT

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQAMIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSL
LKLNQHVAFCDEDHYASLIFVLSAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD

Sequences:

>Translated_147_residues
MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQAMIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSL
LKLNQHVAFCDEDHYASLIFVLSAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD
>Mature_146_residues
SLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQAMIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLL
KLNQHVAFCDEDHYASLIFVLSAIDNHSHLSVLQNLATVLGDNQTVQQLLTAKNAQDIKNILKEHD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789302, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI2367359, Length=135, Percent_Identity=27.4074074074074, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1790755, Length=124, Percent_Identity=30.6451612903226, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16327; Mature: 16196

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQAMIDSVNELGPYIVIAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
EIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVLSAIDNHSHLSVLQNLATV
CEEEEECCCCCHHHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGDNQTVQQLLTAKNAQDIKNILKEHD
HCCCHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
SLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQAMIDSVNELGPYIVIAP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
EIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVLSAIDNHSHLSVLQNLATV
CEEEEECCCCCHHHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGDNQTVQQLLTAKNAQDIKNILKEHD
HCCCHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]