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The map label for this gene is yqiG [H]

Identifier: 148266809

GI number: 148266809

Start: 405454

End: 406653

Strand: Reverse

Name: yqiG [H]

Synonym: SaurJH9_0370

Alternate gene names: 148266809

Gene position: 406653-405454 (Counterclockwise)

Preceding gene: 148266817

Following gene: 148266808

Centisome position: 13.99

GC content: 39.5

Gene sequence:

>1200_bases
ATGTACCGATACAAACCACTTTTACAATCAATCCACTTACCTAATGGCATCAAAATTTCGAATCGCTTTGTATTATCACC
AATGACCGTAAACGCATCAACAAAAGAAGGCTATATTACAAAAGCAGACTTGGCTTATGCTGCACGTCGCTCTAATTCAG
CTGGAATGCAAGTCACCGGCGCTGCTTACATTGAACCTTATGGTAAATTGTTTGAATATGGCTTTAATATTGATCATGAT
GCTTGTATCCCTGGATTAACTAACATGGCATCTACGATGAAACAACATGGTAGCCTCGCTATTATCCAGCTAGCGCATGC
TGGTCGTTTTTCAAATCAAGCCATTTTAAATTTTGGAAAGGTGTATGGACCAAGTCCTATGACTTTGCATTCACCGATTG
AACACGTTGTTATCGCCATGTCTCATGAGAAAATCAATAGCATCATTCAGCAGTATCGCGATGCTACATTACGCGCGATT
AAAGCAGGTTTTGATGGTGTGGAAATTTCGATTGCACAACGTTTGCTGATTCAAACATTTTTCTCAACTTTTTCAAATAG
ACGTACAGACCATTACGGTGCCGACTCATTAAAAAATCGTGCAAGATTATGTTTAGAAGTTATGCGTGCGGTACAAGAAG
TGATTGATAAAGAAGCACCTGACAATTTTATTTTAGGATTTCGAGCAACGCCAGAAGAAACCAGAGGCAGTGATTTAGGA
TATACCATTGACGAGTTCAACCAATTAATCGATTGGGTCATGGACGTTTCAAACATCCAATACTTAGCAATCGCTAGCTG
GGGTCGACATATTTATCAAAATACGTCTCGTACACCAGGTGATCATTTCGGTCGACCAGTTAACCAAATTGTCTATGAAC
ATTTAGCAGGTCGTATCCCTTTAATTGCAAGTGGCGGGATTAATTCTCCAGAGTCTGCTTTAGATGCTTTACAACATGCA
GATATGGTCGGCATGTCTTCGCCTTTTGTCACAGAACCTGACTTTGTGCATAAACTTGCTGAACAGCGACCACATGATAT
CAATCTTGAATTTTCTATGGCAGATTTAGAAGATTTAGCCATTCCACATGCTGCTTTTAAAGATATTGTTAAAATGATGG
ATTATGGGGAAGGTCTAAAGAAGCATACCCGTGACGCTTTACGTCAATTGGAACAGAATTATCATGACTCATCGTCTTAA

Upstream 100 bases:

>100_bases
TCCATGTACCAATTCTATTCTATCAAGACTGTTCATCATTGATTATTCATTCTTTCCATGGTACTTTGAAAACAATATTG
AATAGAAAAGGAATCATAGT

Downstream 100 bases:

>100_bases
GACTATTTTTCATGGAAATTTAAGACAGTGTTATAATGACATCAATATCAAAAATAAGGAGCCTATTAAATCGTGACTGA
AATTAAATATAAAGTAATTA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: dimethylamine; formaldehyde; reduced electron-transferring flavoprotein

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADLAYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

Sequences:

>Translated_399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADLAYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
>Mature_399_residues
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADLAYAARRSNSAGMQVTGAAYIEPYGKLFEYGFNIDHD
ACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAI
KAGFDGVEISIAQRLLIQTFFSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
YTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHA
DMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=308, Percent_Identity=28.2467532467532, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787939, Length=349, Percent_Identity=25.7879656160458, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=429, Percent_Identity=25.1748251748252, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=276, Percent_Identity=26.4492753623188, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=363, Percent_Identity=25.068870523416, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=374, Percent_Identity=24.331550802139, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=362, Percent_Identity=25.6906077348066, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564188, Length=374, Percent_Identity=23.2620320855615, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=362, Percent_Identity=25.1381215469613, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=368, Percent_Identity=25, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.5.8.2

Molecular weight: Translated: 44617; Mature: 44617

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADLAYAARRSNSAGMQVTG
CCCCHHHHHHCCCCCCCEECCEEEECCEEECCCCCCCCEEHHHHHHHHHCCCCCCCEEEC
AAYIEPYGKLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGK
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
VYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTF
HCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
YTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIP
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCC
LIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLA
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHH
IPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADLAYAARRSNSAGMQVTG
CCCCHHHHHHCCCCCCCEECCEEEECCEEECCCCCCCCEEHHHHHHHHHCCCCCCCEEEC
AAYIEPYGKLFEYGFNIDHDACIPGLTNMASTMKQHGSLAIIQLAHAGRFSNQAILNFGK
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
VYGPSPMTLHSPIEHVVIAMSHEKINSIIQQYRDATLRAIKAGFDGVEISIAQRLLIQTF
HCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FSTFSNRRTDHYGADSLKNRARLCLEVMRAVQEVIDKEAPDNFILGFRATPEETRGSDLG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCC
YTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTSRTPGDHFGRPVNQIVYEHLAGRIP
CCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCC
LIASGGINSPESALDALQHADMVGMSSPFVTEPDFVHKLAEQRPHDINLEFSMADLEDLA
EEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHH
IPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQNYHDSSS
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine; H2O; electron-transferring flavoprotein

Specific reaction: trimethylamine + H2O + electron-transferring flavoprotein = dimethylamine + formaldehyde + reduced electron-transferring flavoprotein

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]