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The map label for this gene is aceB [H]

Identifier: 147675709

GI number: 147675709

Start: 279659

End: 281329

Strand: Direct

Name: aceB [H]

Synonym: VC0395_A0266

Alternate gene names: 147675709

Gene position: 279659-281329 (Clockwise)

Preceding gene: 147673590

Following gene: 147675086

Centisome position: 9.25

GC content: 49.37

Gene sequence:

>1671_bases
ATGTTCTTGGTGAGTGTTTCAAGGAAGGAATTTATGTCAATCAATACTGCTGAATTAACCCAACACACCACGGAAACCAA
AATCACCCAAGGCAAGCTTGCTGTGGTGGGTACCCTTTCACCCGAACATCAAGCGATTTTCCCTGTCGAAGCCCAAACTT
TTTTACAGCAACTCTGTGAGCGCTTTGCGCCGCGAGTTGATGAATTGCTGAACTTGCGTGAACAAAAACAAACGCTTATC
GACCAAGGCCAGCTACCTGATTTTCTTGCCGAAACCAAAGATATCCGTGAAGGAAGCTGGAAGATTCTCGGCATTCCAGA
CGATCTGCAAGATCGTCGCGTAGAGATCACGGGGCCAATCGATCGCAAAATGGTGATTAATGCACTGAACGCGAATGTGA
AAGTCTTTATGGCTGACTTTGAAGATTCGATGTCGCCCGCGTGGGACAAGGTGTTGGATGGGCAGATCAACCTGCGTGAT
GCGATTCTGGGCTGCATAAGCTATACCAACCCAGATAATGGCAAGCGTTATGAGCTCAATGCCAACCCAGCGGTGCTTAT
CTGCCGCGTGCGCGGTCTGCATCTCAAAGAAAAACATGTCACTTACAACGGCTTGGCGATTCCCAGTTCACTGTTCGATT
TTGCGCTCTATTTTTACAACAACCATCGCGCACTGTTGAAAAAAGGCAGTGGCCCTTATTTCTACCTACCTAAGCTGCAA
GCTTATCAAGAGGCGCAGTGGTGGAGTGACGTGTTCCATTTCACGGAAGATTATTTTGGTTTAGACACAGGCACCATCAA
AGCAACAGTGTTGATTGAAACTCTGCCTGCGGTATTTCAGATGGATGAGATCCTCTTCTCACTCAAAGAGCACATTGTCG
GTCTGAACTGTGGACGCTGGGATTATATTTTCAGCTACATCAAAACCTTGAAAAAGCATCCCGACCGCGTATTGCCGGAT
CGCCAAGTGGTGACCATGGATAAGCCTTTCCTCAATGCGTATTCCCGTTTGTTGATTTACACCTGTCATAAACGCGGCGC
GTTTGCGATGGGCGGGATGGCGGCGTTTATTCCAGCCAAAGATCCTGCGCTCAATGAGCAAGTGCTACAAAAAATTCGCG
GCGACAAAATGCTTGAAGCGACCAATGGCCACGATGGCACTTGGGTTGCTCACCCCGGTTTAGCGGATACCGCGATGGCG
GTGTTTAACGAAGTGCTTGGTGAGCGCAAAAACCAACTCAATGTAAGCCGAATGGCCGATGCTCCGATAACCGCGGCCGA
TCTATTGGCACCTTGCGATGGGCCACGCTCTGAGCATGGCATGCGCCACAACATTCGCGTTGCGTTGCAATACATCGAAG
CATGGATTTCTGGTAACGGTTGTGTGCCGATCTACGGCTTGATGGAAGATGCGGCGACAGCGGAGATTTCACGCGCTTCG
ATTTGGCAATGGATCCAACACGGCAAAACGTTAGATAACGGGCAAGTGGTCACTAACGAACTGTTTCGCGACTACCTCAA
ACAAGAGATTGAGGTGGTGAAAAGTGAAGTGGGTGAGTCCCGTTTTGCACAAGGGCGTTTTACTGAAGCGGCCGAATTGA
TGGAGCGCTTAACGACCAGTCAAGAGCTACCGAATTTCTTAACCATACCGGGCTACGACTACTTGCCGTAG

Upstream 100 bases:

>100_bases
CTAGTGCCCGGAGTCTTGCAAAAGTGCGCAATTTAAGTGACTTTAAATCACAACGGATAAGCCAACTGAATAATGAAAAT
TTGTAGTTTTAAAGTTACAA

Downstream 100 bases:

>100_bases
GCCTCTATAACACCAGTAAGGTGAAAAAACACCAGTAACGTGAAACCACTTTACGATCAGCGAGCGCAGTACTGATCGAA
CAAGGAATCGAGCTGCCAAC

Product: malate synthase

Products: NA

Alternate protein names: MSA [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLNLREQKQTLI
DQGQLPDFLAETKDIREGSWKILGIPDDLQDRRVEITGPIDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGCISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPSSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAADLLAPCDGPRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP

Sequences:

>Translated_556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLNLREQKQTLI
DQGQLPDFLAETKDIREGSWKILGIPDDLQDRRVEITGPIDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGCISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPSSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAADLLAPCDGPRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP
>Mature_556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLNLREQKQTLI
DQGQLPDFLAETKDIREGSWKILGIPDDLQDRRVEITGPIDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGCISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPSSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAADLLAPCDGPRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=532, Percent_Identity=71.0526315789474, Blast_Score=791, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17561814, Length=515, Percent_Identity=50.873786407767, Blast_Score=500, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71982926, Length=409, Percent_Identity=53.3007334963325, Blast_Score=409, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324212, Length=519, Percent_Identity=46.6281310211946, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322222, Length=519, Percent_Identity=45.4720616570328, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 62900; Mature: 62900

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCE
CEEEEECHHHHHCCCHHHHHHHHHHHHEECCCEEEEEECCCCCCEEECCHHHHHHHHHHH
RFAPRVDELLNLREQKQTLIDQGQLPDFLAETKDIREGSWKILGIPDDLQDRRVEITGPI
HHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCC
DRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRDAILGCISYTNPDNGKRYELN
CHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEC
ANPAVLICRVRGLHLKEKHVTYNGLAIPSSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
CCCEEEEEEECCCEEECCEEEECCEECHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHH
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRW
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DYIFSYIKTLKKHPDRVLPDRQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAK
HHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCEEECCEEEECCCC
DPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMAVFNEVLGERKNQLNVSRMAD
CCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
APITAADLLAPCDGPRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
QELPNFLTIPGYDYLP
HHCCCEEECCCCCCCC
>Mature Secondary Structure
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCE
CEEEEECHHHHHCCCHHHHHHHHHHHHEECCCEEEEEECCCCCCEEECCHHHHHHHHHHH
RFAPRVDELLNLREQKQTLIDQGQLPDFLAETKDIREGSWKILGIPDDLQDRRVEITGPI
HHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCC
DRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRDAILGCISYTNPDNGKRYELN
CHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEC
ANPAVLICRVRGLHLKEKHVTYNGLAIPSSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
CCCEEEEEEECCCEEECCEEEECCEECHHHHHHHHHHHCCCCCEEEECCCCCEEECCCHH
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRW
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DYIFSYIKTLKKHPDRVLPDRQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAK
HHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCEEECCEEEECCCC
DPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMAVFNEVLGERKNQLNVSRMAD
CCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
APITAADLLAPCDGPRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
QELPNFLTIPGYDYLP
HHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3050899; 3060852; 8265357; 9278503 [H]