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The map label for this gene is aceE [H]

Identifier: 147675572

GI number: 147675572

Start: 2138133

End: 2140793

Strand: Reverse

Name: aceE [H]

Synonym: VC0395_A1990

Alternate gene names: 147675572

Gene position: 2140793-2138133 (Counterclockwise)

Preceding gene: 147673255

Following gene: 147674758

Centisome position: 70.79

GC content: 50.85

Gene sequence:

>2661_bases
ATGTCTGACATGAAGCATGACGTAGATGCACTGGAAACTCAGGAGTGGCTTGCTGCCCTTGAATCCGTTGTCCGTGAAGA
AGGCGTAGAGCGTGCGCAGTACCTGCTTGAACAAGTACTGGAAAAAGCGCGTTTGGATGGGGTAGACATGCCAACAGGCG
TGACCACCAACTACATCAACACGATTCCTGCGGCGCAGGAGCCTGCTTACCCAGGTGACACCACGATCGAACGTCGTATC
CGTTCAATCATTCGCTGGAACGCGATCATGATCGTGCTGCGTGCATCGAAGAAAGATCTGGAACTGGGTGGCCACATGGC
TTCTTTCCAATCTTCCGCTGCTTTCTACGAAACCTGTTTCAACCACTTCTTCCGTGCTCCAAACGAGAAAGACGGTGGCG
ACTTGGTTTACTATCAAGGTCACATCTCTCCAGGGATCTACGCGCGTGCTTTCGTTGAAGGCCGTTTGACCGAAGAGCAA
CTGGACAACTTCCGTCAGGAAGTGGATGGCAAAGGTCTTCCTTCTTACCCACACCCGAAATTGATGCCTGAATTCTGGCA
ATTCCCAACCGTATCTATGGGTCTGGGTCCAATTTCTGCGATTTATCAAGCTCGTTTCCTGAAGTATCTGAATGGTCGTG
GCCTGAAAGACACCACGGCACAGCGCGTATACGCCTTCCTTGGTGACGGTGAGATGGACGAGCCAGAATCACGCGGCGCG
ATCTCTTTTGCTGCGCGTGAGAAGCTGGATAACCTGTGCTTCCTGATCAACTGTAACCTGCAACGTCTGGACGGCCCTGT
TATGGGTAACGGCAAGATCATCCAAGAACTGGAAGGTCTGTTCCGTGGCGCAGGCTGGAACGTTGTGAAAGTAATCTGGG
GTAATGGCTGGGACAAACTACTGGCGAAAGATACCACAGGTAAACTGCTGCAACTGATGAACGAAACCATCGACGGCGAC
TACCAAACGTTCAAAGCGAAAGATGGCGCTTACGTTCGTGAGCACTTCTTCGGTAAGTACCCAGAGACTGCAGCACTGGT
TGCTGACATGACTGACGATGAGATCTTCGCTCTGAAACGCGGTGGTCACGAATCATCTAAACTGTACGCGGCGTTCAAGA
ACGCTCAAGACACCAAAGGTCGTCCAACCGTTATCCTAGCGAAAACCGTTAAAGGTTACGGCATGGGTGATGCGGCAGAA
GGTAAGAACATTGCGCACCAAGTGAAGAAGATGGATATGACCCATGTTCTGGCGATGCGTAACCGTCTAGGCCTGCAAGA
TCTGATCTCTGATGAAGAAGTGAAGAACCTGCCGTACCTGAAACTGGAAGAAGGCTCAAAAGAGTTTGAATACCTGCACG
CTCGTCGTAAAGCACTGCATGGTTACACACCTCAGCGTCTACCTAACTTCACGGGTGAGTTAGTGATCCCTGCGCTGGAA
GAGTTCAAGCCACTGCTGGAAGAGCAGAGTCGCGAAATTTCTTCAACTATGGCGTATGTACGTACTCTGAACATCCTGCT
GAAAGATAAGAACATTGGTCAAAACATCGTTCCTATCATCGCGGACGAAGCGCGTACGTTCGGTATGGAAGGTCTGTTCC
GTCAAATCGGTATCTACAACCCGCATGGTCAGAACTACACTCCACAAGATCGCGATATCGTTTCTTACTACAAAGAAGCG
ACGTCAGGTCAGGTACTGCAAGAAGGTATCAACGAGCTGGGTGCCATGTCATCTTGGGTTGCGGCGGCAACGTCATACAG
CACCAACAACCTGCCGATGATCCCGTTCTACATCTACTACTCTATGTTCGGTTTCCAACGTGTTGGCGACATGGCGTGGA
TGGCGGGCGACCAACAAGCGCGTGGCTTCCTACTGGGTGCTACTGCGGGTCGTACCACGCTGAACGGTGAAGGTCTACAG
CACGAAGATGGTCACTCGCACATTCTGGCGGGCACAGTACCAAACTGTATCTCTTACGATCCAACCTTCGCTTACGAAGT
TGCGGTAATCCTGCAAGATGGTATCCGTCGCATGTACGGTGAGCAAGAGAACGTGTTCTACTACCTGACACTGATGAACG
AAAGCTACGCTCACCCAGCAATGCCTGCTGGCGCTGAAGAAGGTATCCGTAAAGGTATCTACAAGCTAGAAACGCACGCT
GGTAACAAAGCAAAAGTTCAACTGATGAGCTCAGGCACCATCATGAACGAAGTACGTAAAGCGGCACAAATTCTGAGTGA
AGAGTACGGCGTAGCGTCTGACGTTTACTCTGTGACTTCATTCAACGAGCTGGCGCGTGATGGCCAAGCGTGTGATCGTT
TCAACATGCTGCACCCAGAAGCAGAGGTGAAAGTACCTTACATCGCACAAGTGATGGGTACTGAGCCTGCTATCGCAGCG
ACTGACTACATGAAGAACTACGCGGATCAAGTACGTGCGTTCATTCCTGCACAGTCTTACAAAGTGCTGGGTACGGATGG
TTTCGGCCGCTCAGACAGCCGTGAAAACCTACGTCGTCACTTTGAAGTGAATGCTGGCTACGTGGTAGTTGCAGCGCTGA
ATGAACTGGCAAAACGTGGTGAAGTTGAGAAGTCTGTGGTTGCAGCAGCAATCAAGAAATTCGACATCGATACTGAAAAA
ACCAACCCGCTGTACGCTTAA

Upstream 100 bases:

>100_bases
CGCCGTGAGCGATCTCTGCGTCGAATTCAACAAGGTAATGAGCCGCAATAGCGGCCATTGCGATTCAACTTAAATCCAAC
CAACAGAAGGATAGATCGCC

Downstream 100 bases:

>100_bases
TTGAAGGTAGGAAAAGTAATGGCAATCGAAATTTATGTACCTGACATCGGTGCGGATGAGGTTGAAGTCACTGAGATTCT
CGTCAAAGTCGGCGACAAAG

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MSDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYINTIPAAQEPAYPGDTTIERRI
RSIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCFNHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQ
LDNFRQEVDGKGLPSYPHPKLMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGA
ISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKLLAKDTTGKLLQLMNETIDGD
YQTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKRGGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAE
GKNIAHQVKKMDMTHVLAMRNRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGELVIPALE
EFKPLLEEQSREISSTMAYVRTLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYNPHGQNYTPQDRDIVSYYKEA
TSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQ
HEDGHSHILAGTVPNCISYDPTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHA
GNKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPEAEVKVPYIAQVMGTEPAIAA
TDYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRHFEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEK
TNPLYA

Sequences:

>Translated_886_residues
MSDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYINTIPAAQEPAYPGDTTIERRI
RSIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCFNHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQ
LDNFRQEVDGKGLPSYPHPKLMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGA
ISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKLLAKDTTGKLLQLMNETIDGD
YQTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKRGGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAE
GKNIAHQVKKMDMTHVLAMRNRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGELVIPALE
EFKPLLEEQSREISSTMAYVRTLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYNPHGQNYTPQDRDIVSYYKEA
TSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQ
HEDGHSHILAGTVPNCISYDPTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHA
GNKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPEAEVKVPYIAQVMGTEPAIAA
TDYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRHFEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEK
TNPLYA
>Mature_885_residues
SDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYINTIPAAQEPAYPGDTTIERRIR
SIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCFNHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQL
DNFRQEVDGKGLPSYPHPKLMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGAI
SFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKLLAKDTTGKLLQLMNETIDGDY
QTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKRGGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAEG
KNIAHQVKKMDMTHVLAMRNRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGELVIPALEE
FKPLLEEQSREISSTMAYVRTLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYNPHGQNYTPQDRDIVSYYKEAT
SGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYYSMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQH
EDGHSHILAGTVPNCISYDPTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHAG
NKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPEAEVKVPYIAQVMGTEPAIAAT
DYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRHFEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEKT
NPLYA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=883, Percent_Identity=73.2729331823329, Blast_Score=1383, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17539652, Length=226, Percent_Identity=28.7610619469027, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 99009; Mature: 98877

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYIN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
TIPAAQEPAYPGDTTIERRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHH
NHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQLDNFRQEVDGKGLPSYPHPK
HHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
LMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGA
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
ISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKL
CHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHH
LAKDTTGKLLQLMNETIDGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKR
HHCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEC
GGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAEGKNIAHQVKKMDMTHVLAMR
CCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGELVIPALE
HHCCHHHHHCCHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCEEHHHHH
EFKPLLEEQSREISSTMAYVRTLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYN
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHCHHHHHHHHCCCC
PHGQNYTPQDRDIVSYYKEATSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQHEDGHSHILAGTVPNCISYD
HHHHHHHHCCHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
PTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCHHHHHCCC
GNKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPE
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
AEVKVPYIAQVMGTEPAIAATDYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRH
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHH
FEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEKTNPLYA
EECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDMKHDVDALETQEWLAALESVVREEGVERAQYLLEQVLEKARLDGVDMPTGVTTNYIN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
TIPAAQEPAYPGDTTIERRIRSIIRWNAIMIVLRASKKDLELGGHMASFQSSAAFYETCF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHH
NHFFRAPNEKDGGDLVYYQGHISPGIYARAFVEGRLTEEQLDNFRQEVDGKGLPSYPHPK
HHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
LMPEFWQFPTVSMGLGPISAIYQARFLKYLNGRGLKDTTAQRVYAFLGDGEMDEPESRGA
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
ISFAAREKLDNLCFLINCNLQRLDGPVMGNGKIIQELEGLFRGAGWNVVKVIWGNGWDKL
CHHHHHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHH
LAKDTTGKLLQLMNETIDGDYQTFKAKDGAYVREHFFGKYPETAALVADMTDDEIFALKR
HHCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCCCCEEEEECCCCCEEEEEC
GGHESSKLYAAFKNAQDTKGRPTVILAKTVKGYGMGDAAEGKNIAHQVKKMDMTHVLAMR
CCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NRLGLQDLISDEEVKNLPYLKLEEGSKEFEYLHARRKALHGYTPQRLPNFTGELVIPALE
HHCCHHHHHCCHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCEEHHHHH
EFKPLLEEQSREISSTMAYVRTLNILLKDKNIGQNIVPIIADEARTFGMEGLFRQIGIYN
HHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEECHHHHHCHHHHHHHHCCCC
PHGQNYTPQDRDIVSYYKEATSGQVLQEGINELGAMSSWVAAATSYSTNNLPMIPFYIYY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SMFGFQRVGDMAWMAGDQQARGFLLGATAGRTTLNGEGLQHEDGHSHILAGTVPNCISYD
HHHHHHHHCCHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
PTFAYEVAVILQDGIRRMYGEQENVFYYLTLMNESYAHPAMPAGAEEGIRKGIYKLETHA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHCHHHHHCCC
GNKAKVQLMSSGTIMNEVRKAAQILSEEYGVASDVYSVTSFNELARDGQACDRFNMLHPE
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
AEVKVPYIAQVMGTEPAIAATDYMKNYADQVRAFIPAQSYKVLGTDGFGRSDSRENLRRH
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHH
FEVNAGYVVVAALNELAKRGEVEKSVVAAAIKKFDIDTEKTNPLYA
EECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]