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The map label for this gene is rlmN

Identifier: 147675306

GI number: 147675306

Start: 299279

End: 300400

Strand: Direct

Name: rlmN

Synonym: VC0395_A0286

Alternate gene names: 147675306

Gene position: 299279-300400 (Clockwise)

Preceding gene: 147673453

Following gene: 147675566

Centisome position: 9.9

GC content: 47.86

Gene sequence:

>1122_bases
ATGACCACAGAAAAAGTCAATCTACTCGATTTTGACCGCAAGGGTTTGCGCACGTTTTTTGCCGAAGAACTGGGTGAGAA
AGCGTTTCGTGCCGAGCAGGTCATGAAGTGGATTTATCACTTTGGTTGTGATGACTTCGATCAAATGAATAACATCAACA
AGCAGCTGCGTGAAAAGCTCAAAGCCAAGTGTGAGATCCGTGCACCTTATGTCTCGGAAGCGCAACACTCGGCGGATGGC
ACCATCAAGTGGGCAATGCGTGTGGGTGATCAGGATGTAGAAACGGTTTACATTCCCGAAGATGATCGCGCGACCTTATG
TGTATCTTCCCAAGTAGGGTGTGCGTTGGAATGCAAATTCTGCTCAACGGCGCAGCAAGGTTTTAACCGTAACCTACGCG
TTTCAGAAATCATTGGTCAGGTTTGGCGTGCGGCACGTGAAATCGGTCTAGAGAAAGAAACTGGGCGTCGTCCTATCACT
AACGTGGTGATGATGGGCATGGGTGAGCCTTTGCTCAACATGAAAAACCTCATCCCAGCGTTAGAGATCATGCTCGATGA
TCTCGGTTTTGGTCTTTCTAAGCGCCGTGTGACCGTTTCTACCTCTGGTGTGGTTTCTGGTTTAGAGCAGATGATTGGCC
AAATCGATGTGGCTTTGGCTATCTCATTGCATGCGCCGAATGACAAGCTGCGTAGCGAAATCATGCCGATCAACGATCGC
TGGAATATCGAAGCGTTTTTAGAGGTGGTGCGTCGCTATATTGCATCCTCAAATGCTAACCGCGGTAAGGTGACCGTTGA
GTATGTGCTGCTGGATCATGTGAACGATGGTACAGAGCATGCTCACGAGTTGGCGGAATTACTGAAAAGGACGCCTTGTA
AAATTAACCTGATCCCGTTTAACCCTTATCCGGGCTCACCCTACAAGAAGCCAAGTAATTCACGCATCGACCGTTTCCAG
AAAACCTTGATGCAATATGAGCATACCGTCACCATTCGTAAAACTCGTGGTGATGACATTGATGCGGCATGTGGGCAGTT
GGTGGGAGATGTCATTGATCGCACTAAACGAACCAAAGCTAAACAATTTGATGGACAAGCGATTCCAGTGAAAACACTGT
AA

Upstream 100 bases:

>100_bases
ACACCTTAGAAAAGACGCGGTTTTGCAGCCCTTGTTGTTGCTGAATAAAGGCTGTACAATTCGCGCCCTTAATTACTGTT
CCGTCTTCCTGAGAGGCCCC

Downstream 100 bases:

>100_bases
TTGATGTTATAAGATAAGCCAGAGATGTTTCTCTGGCTTTATTTTTGCCTAATATGGCAACAAATCACTGCGTTTAAATG
TCAAATCAAAGAAAAACCTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADG
TIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKRTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL

Sequences:

>Translated_373_residues
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADG
TIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPIT
NVVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKRTPCKINLIPFNPYPGSPYKKPSNSRIDRFQ
KTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL
>Mature_372_residues
TTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKLKAKCEIRAPYVSEAQHSADGT
IKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITN
VVMMGMGEPLLNMKNLIPALEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDRW
NIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKRTPCKINLIPFNPYPGSPYKKPSNSRIDRFQK
TLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKAKQFDGQAIPVKTL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=369, Percent_Identity=73.4417344173442, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_VIBC3 (A5F3F8)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3F8
- SMR:   A5F3F8
- STRING:   A5F3F8
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0286
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- BioCyc:   VCHO345073:VC0395_A0286-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 42180; Mature: 42049

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKL
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KAKCEIRAPYVSEAQHSADGTIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKF
HHCCEECCCCHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCC
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKRTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQFDGQAIPVKTL
HHCCCCCCCCCCC
>Mature Secondary Structure 
TTEKVNLLDFDRKGLRTFFAEELGEKAFRAEQVMKWIYHFGCDDFDQMNNINKQLREKL
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KAKCEIRAPYVSEAQHSADGTIKWAMRVGDQDVETVYIPEDDRATLCVSSQVGCALECKF
HHCCEECCCCHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEHH
CSTAQQGFNRNLRVSEIIGQVWRAAREIGLEKETGRRPITNVVMMGMGEPLLNMKNLIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHCCCCHHHHHHHHHHH
LEIMLDDLGFGLSKRRVTVSTSGVVSGLEQMIGQIDVALAISLHAPNDKLRSEIMPINDR
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCC
WNIEAFLEVVRRYIASSNANRGKVTVEYVLLDHVNDGTEHAHELAELLKRTPCKINLIPF
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYKKPSNSRIDRFQKTLMQYEHTVTIRKTRGDDIDAACGQLVGDVIDRTKRTKA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KQFDGQAIPVKTL
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA