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The map label for this gene is mraW [H]

Identifier: 147675217

GI number: 147675217

Start: 2132817

End: 2133767

Strand: Reverse

Name: mraW [H]

Synonym: VC0395_A1987

Alternate gene names: 147675217

Gene position: 2133767-2132817 (Counterclockwise)

Preceding gene: 147674273

Following gene: 147674513

Centisome position: 70.56

GC content: 50.89

Gene sequence:

>951_bases
ATGACTGCTTCTTTCCAACACATCTCTGTATTATTGAACGAATCGATTGAAGGATTGGCGATCAAGCCGGATGGTATCTA
CATCGATGGCACCTTTGGCCGCGGTGGTCACAGTCGCACCATTCTCGCTCAGTTAGGCCCAGAAGGTCGTCTGTACAGCA
TTGACCGTGATCCGCAAGCGATTGCCGAAGCGCAGAAGATTGATGATCCGCGTTTTACCATCGTCCATGGCCCTTTCTCG
GGCATTGCCGAATACGCACAGCGTTATGATCTGGTGGGTAAAGTCGATGGTGTGTTGTTCGATCTCGGCGTTTCTTCACC
GCAGTTGGATGATGCTGAGCGCGGTTTTAGTTTTATGAAAGATGGTCCACTCGACATGCGTATGGATCCAACCAGTGGTA
TGCCAGTTTCCGCGTGGTTGGCACAAGCCGACTTGGATGACATCACTTGGGTGATTCGCGAGTTTGGTGAAGATAAACAT
GCGCGCAGAATAGCTAAAGCCATTGTCGAATATCGGGAAAACGAGCTCAACGAGCCCTTGACGCGTACTAGCCAGTTAGC
AAAACTCATCTCCGACGCGGCGCCTAAAAGTTTCAAAGAGAAAAAGCACCCTGCCACCCGCGCTTTTCAAGCTTTTCGGA
TCTACATCAACAGTGAATTAGAAGAGATTGATACCGCATTGCGCGGCGCTTCTGACATTCTGGCTCCACAAGGCCGCCTG
TCCGTGATCAGTTTCCATTCACTAGAAGATCGCATGGTGAAACGCTTTATGCGTAAAGAGAGCCAAGGCCCAGAAGTGCC
ACACGGCCTTCCTTTGACCCAAGAGCAAATTCGTGCGCTCGGCAGTGCCAAGATGAAAACTGTGGGTAAGGCACTCAAGC
CATCGGATCAAGAAGTCGAACTTAACCCGCGTTCACGCAGCTCTGTTCTGCGCGTGGCAGAAAAACTGTAG

Upstream 100 bases:

>100_bases
AGCGCAAAGTCACGTCCTGTATACGCTTATGGCTAGCGGTAGTGTCTGAAATCGGTACACTAACCCATATGACGGGCATG
CCCAGCACAACGAGAAAATC

Downstream 100 bases:

>100_bases
AGGGTTACTATGCCTCGTCAGTCGCCGCCGAACCTCGCGAAGTTAATTGCCCTCGATCTACTGACGGTAGGTCGGGTGCC
CTTGCTGCTTTTAGTGCTGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTASFQHISVLLNESIEGLAIKPDGIYIDGTFGRGGHSRTILAQLGPEGRLYSIDRDPQAIAEAQKIDDPRFTIVHGPFS
GIAEYAQRYDLVGKVDGVLFDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGMPVSAWLAQADLDDITWVIREFGEDKH
ARRIAKAIVEYRENELNEPLTRTSQLAKLISDAAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALRGASDILAPQGRL
SVISFHSLEDRMVKRFMRKESQGPEVPHGLPLTQEQIRALGSAKMKTVGKALKPSDQEVELNPRSRSSVLRVAEKL

Sequences:

>Translated_316_residues
MTASFQHISVLLNESIEGLAIKPDGIYIDGTFGRGGHSRTILAQLGPEGRLYSIDRDPQAIAEAQKIDDPRFTIVHGPFS
GIAEYAQRYDLVGKVDGVLFDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGMPVSAWLAQADLDDITWVIREFGEDKH
ARRIAKAIVEYRENELNEPLTRTSQLAKLISDAAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALRGASDILAPQGRL
SVISFHSLEDRMVKRFMRKESQGPEVPHGLPLTQEQIRALGSAKMKTVGKALKPSDQEVELNPRSRSSVLRVAEKL
>Mature_315_residues
TASFQHISVLLNESIEGLAIKPDGIYIDGTFGRGGHSRTILAQLGPEGRLYSIDRDPQAIAEAQKIDDPRFTIVHGPFSG
IAEYAQRYDLVGKVDGVLFDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTSGMPVSAWLAQADLDDITWVIREFGEDKHA
RRIAKAIVEYRENELNEPLTRTSQLAKLISDAAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALRGASDILAPQGRLS
VISFHSLEDRMVKRFMRKESQGPEVPHGLPLTQEQIRALGSAKMKTVGKALKPSDQEVELNPRSRSSVLRVAEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=342, Percent_Identity=40.3508771929825, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=40.7035175879397, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=62.6582278481013, Blast_Score=416, Evalue=1e-118,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=37.6506024096386, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=37.6506024096386, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35127; Mature: 34996

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASFQHISVLLNESIEGLAIKPDGIYIDGTFGRGGHSRTILAQLGPEGRLYSIDRDPQA
CCCCHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCHHH
IAEAQKIDDPRFTIVHGPFSGIAEYAQRYDLVGKVDGVLFDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTSGMPVSAWLAQADLDDITWVIREFGEDKHARRIAKAIVEYRENELNEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
TRTSQLAKLISDAAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALRGASDILAPQGRL
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCCE
SVISFHSLEDRMVKRFMRKESQGPEVPHGLPLTQEQIRALGSAKMKTVGKALKPSDQEVE
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LNPRSRSSVLRVAEKL
ECCCHHHHHHHHHHCC
>Mature Secondary Structure 
TASFQHISVLLNESIEGLAIKPDGIYIDGTFGRGGHSRTILAQLGPEGRLYSIDRDPQA
CCCHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCEEEEEECCCCCEEEECCCHHH
IAEAQKIDDPRFTIVHGPFSGIAEYAQRYDLVGKVDGVLFDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTSGMPVSAWLAQADLDDITWVIREFGEDKHARRIAKAIVEYRENELNEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
TRTSQLAKLISDAAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALRGASDILAPQGRL
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCCE
SVISFHSLEDRMVKRFMRKESQGPEVPHGLPLTQEQIRALGSAKMKTVGKALKPSDQEVE
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
LNPRSRSSVLRVAEKL
ECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]